Drug-loaded KGN nanofiber scaffold and preparation method and application thereof

A nanofiber and drug-loaded technology, applied in medical science, prostheses, coatings, etc., can solve the problems of unsatisfactory biological functions, insufficient self-healing and guiding capabilities, and achieve great application potential, promote transformation, and reduce inflammatory reactions. Effect

Inactive Publication Date: 2020-07-24
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, due to the characteristics of the structure and function of the interface tissue, the ability to guide self-repair after injury is insufficient, and scar vascular tissue is often formed, which cannot meet the biological function of mechanical load transmission

Method used

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  • Drug-loaded KGN nanofiber scaffold and preparation method and application thereof
  • Drug-loaded KGN nanofiber scaffold and preparation method and application thereof
  • Drug-loaded KGN nanofiber scaffold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Prepare a drug-loaded KGN nanofiber scaffold, the preparation process is as follows figure 1 As shown, the specific method is as follows:

[0051] (1) Preparation of SF nanofiber scaffold (PD-SF) with PD modification on the surface:

[0052] a. Put 0.5cm×0.5cm flake cocoons in 0.5% NaHCO 3 After boiling in the aqueous solution for 30 minutes, rinse with double distilled water;

[0053] B, repeat the operation in step a to obtain degummed silk, which is placed in an oven to dry;

[0054] c, place the dried silk in 55g CaCl 2 , 58.2ml of absolute ethanol, and 72ml of triple-distilled water in a ternary system, heated at 70°C for 6 hours to completely dissolve the silk, and obtained a silk fibroin solution with a concentration of 10%, which passed through a membrane with a pore size of 4.5um Filtration, double-distilled water dialysis for 3 days, and double-distilled water was replaced every 6 hours to remove other ions, and the obtained solution was freeze-dried to ob...

Embodiment 2

[0062] A kind of drug-loaded KGN nanofiber scaffold (KGN-PD-SF) prepared in Example 1 is characterized, and the results are as follows figure 2 shown, where figure 2 Middle A is the FTIR spectrum, showing that KGN-PD-SF has a stronger amide absorption peak than PD-SF (PD-modified SF nanofiber scaffold), while KGN-PD-SF has no ammonium salt absorption peak, while figure 2 B is 1 From the H NMR spectrum, it can also be seen that compared with KGN and PD-SF, KGN-PD-SF has the characteristic absorption peak (7.3-7.9ppm) of the KGN benzene ring proton. The above results indicated that KGN and PD-SF were covalently cross-linked through amide bonds in the drug-loaded KGN nanofibrous scaffold (KGN-PD-SF).

[0063] image 3 It is a topographic structure diagram of a drug-loaded KGN nanofiber scaffold (KGN-PD-SF) prepared in SF, PD-SF and Example 1, from which the drug-loaded KGN nanofiber scaffold of the present invention is randomly arranged to form a porous network structure ,...

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Abstract

The invention relates to a drug-loaded KGN nanofiber scaffold and a preparation method and application thereof, and belongs to the field of preparation of biological scaffolds. The invention disclosesthe drug-loaded KGN nanofiber scaffold. The preparation method comprises the following steps: firstly, modifying the surface of silk fibroin (SF) nanofiber with polydopamine (PD), and loading KGN tothe surface of the nanofiber through EDC / NHS amide condensation reaction by utilizing an amino group of the PD and a carboxyl group of an active drug KGN, so that oxidative stress can be eliminated and inflammatory reaction can be reduced in the early stage, and transformation from an inflammatory phase to a tissue regeneration and remodeling phase is promoted; and meanwhile, continuously and slowly releasing the KGN in the middle and later periods, inducing formation of fibrous cartilage tissues, and finally realizing regeneration of interface tissues. In addition, in-vitro experimental results prove that the drug-loaded KGN nanofiber scaffold has an inflammation regulating function, can promote formation of fibrous cartilage of bone-tendon interface tissues, and has great application potential in interface tissue engineering.

Description

technical field [0001] The invention belongs to the field of biological scaffold preparation, in particular to a drug-loaded KGN nanofiber scaffold and its preparation method and application. Background technique [0002] The interface tissue of the tendon-skeletal system can be divided into three major areas, namely: (1) bone; (2) fibrocartilage; (3) tendon / ligament, in which fibrocartilage can minimize stress concentration because of its ability to buffer impact, and then It plays a vital role in the effective transmission of mechanical loads. Daily physical activities can easily cause bone-tendon / ligament and other interface tissue damage, and when the bone and tendon / ligament are directly combined again through surgery, the original tissue structure, biological function and microenvironment of the interface area will be lost, making the two They cannot be effectively integrated with each other, and their stability is poor, and the rate of re-injury is high, which has be...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/34A61L27/50A61L27/54
CPCA61L27/227A61L27/34A61L27/50A61L27/54A61L2300/214A61L2300/41A61L2300/412A61L2300/606A61L2430/06A61L2430/10C08L89/00C08L79/02
Inventor 杨力陈培兴
Owner CHONGQING UNIV
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