Preparation method and application of a magnetic structural color hydrogel microcarrier for multivariate analysis of bladder cancer proteins
A magnetic structure and multiple analysis technology, applied in the field of biomedical materials, can solve problems such as poor repeatability, cross-contamination, and large steric hindrance, and achieve high specificity, high flexibility, and good stability
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Embodiment 1
[0032] Preparation of magnetic structured color hydrogel microcarriers
[0033] (1) see figure 1 In the preparation process, silica nanoparticles with a particle size of 215nm were respectively selected, and a monodisperse silica nanoparticle suspension with a concentration of 20 m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the External phase, the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65°C, the droplets of the silica nanoparticles evaporate with the water And self-assembly, after 12 hours of solidification and crystallization, silicon dioxide photonic crystal microspheres are formed; the temperature is raised to 95° C. to solidify the silicon dioxide photonic crystal microspheres for 2 hours; then the silicon dioxide photonic crystal microspheres are collected in a cru...
Embodiment 2
[0038] Preparation of magnetic structured color hydrogel microcarriers
[0039] (1) see figure 1In the preparation process, silica nanoparticles with a particle size of 237nm were respectively selected, and a monodisperse silica nanoparticle suspension with a concentration of 20 m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the External phase, the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65°C, the droplets of the silica nanoparticles evaporate with the water And self-assembly, after 12 hours of solidification and crystallization, silicon dioxide photonic crystal microspheres are formed; the temperature is raised to 95° C. to solidify the silicon dioxide photonic crystal microspheres for 2 hours; then the silicon dioxide photonic crystal microspheres are collected in a cruc...
Embodiment 3
[0043] Preparation of magnetic structured color hydrogel microcarriers
[0044] (1) see figure 1 In the preparation process, silica nanoparticles with a particle size of 247nm were respectively selected, and a monodisperse silica nanoparticle suspension with a concentration of 20 m / v% was used as the internal phase, and dimethyl fluorinated silicone oil (50cSt) was used as the External phase, the external phase and the internal phase are injected into the corresponding channels of the microfluidic device, collected in the container through the output channel of the microfluidic device, and at 65°C, the droplets of the silica nanoparticles evaporate with the water And self-assembly, after 12 hours of solidification and crystallization, silicon dioxide photonic crystal microspheres are formed; the temperature is raised to 95° C. to solidify the silicon dioxide photonic crystal microspheres for 2 hours; then the silicon dioxide photonic crystal microspheres are collected in a cru...
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