Application of stem cell Sca-1<+> subgroup exosome in preparation of biological agent for repairing retinal injury

A technology for retinal damage and biological agents, applied in the direction of microorganisms, animal cells, vertebrate cells, etc., can solve problems such as differences in active substances, achieve high efficacy, strong clinical transformation potential, and avoid side effects

Inactive Publication Date: 2020-08-07
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although BMCs-derived exosomes are considered as new candidates for cell-free therapy, the active substances contained in exosomes from different cell sources have certain differences

Method used

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  • Application of stem cell Sca-1&lt;+&gt; subgroup exosome in preparation of biological agent for repairing retinal injury
  • Application of stem cell Sca-1&lt;+&gt; subgroup exosome in preparation of biological agent for repairing retinal injury
  • Application of stem cell Sca-1&lt;+&gt; subgroup exosome in preparation of biological agent for repairing retinal injury

Examples

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Embodiment 1

[0021] Example 1 Stem Cell Sca-1 + Application of subpopulation exosomes in retinal injury repair

[0022] Take fresh bone marrow stem cells from C57BL / 6 mice aged 2-3 months, and form a concentration of 1×10 8 cell suspension, according to Sca-1 Positive Cell Screening Kit Description Sorting bone marrow cells, the adsorbed cells are BMCs-Sca-1 + , unadsorbed cells are BMCs-Sca-1 - , and unsorted BMCs were used as control cells, which were suspended in 0.9% saline for later use. Isolated BMCs-Sca-1 + , BMCs-Sca-1 - and BMCs-total were respectively cultured in exosome-free serum medium to a density of about 80%-95%, collected the mixture of cells and medium in each group, extracted and purified exosomes by ultracentrifugation, and centrifuged at 300g at 4°C for 10 Remove the cells and their debris within minutes, take the supernatant, and continue to centrifuge at 3000g at 4°C for 10 minutes to remove residual cell debris; take the supernatant and centrifuge at 10,000g ...

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Abstract

The invention discloses application of stem cell Sca-1<+> subgroup exosome in preparation of a biological agent for repairing retinal injury. The stem cell Sca-1<+> subgroup exosome is prepared by carrying out sorting optimization treatment on BM Cs through a molecular marker Sca-1 and then carrying out culture and extraction. The Sca-1<+> subgroup stem cells have a stronger paracrine effect, thebiological effect of unit exosome can be enhanced, the extracted exosome can increase the survival rate of injured retinal cells and avoid in-vivo immunological rejection, and a new strategy is provided for treatment of ophthalmic diseases such as retinal injury and optic neurodegenerative change.

Description

technical field [0001] The present invention relates to an extracellular membrane vesicle secreted by stem cells, in particular to the stem cell antigen-1 (stemcell antigen-1, Sca-1) that is obtained by screening and optimizing the derived bone marrow stem cells, which has a more potent trophic group It can be used for the repair of damaged retinal tissue. Background technique [0002] Retinal ganglion cells (RGCs) are the common target cells of neurodegenerative diseases such as glaucoma, diabetic retinopathy, and macular degeneration. Under the action of ischemia, trauma, inflammation and other disease factors, RGCs usually produce irreversible damage. It is difficult to regenerate after apoptosis. In addition to the nature of neurons, the external microenvironment after injury seriously affects the repair of RGCs, which eventually leads to damage to the structure and function of the retina, until vision loss. [0003] Exosomes are an important part of the extracellular m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P27/02A61P9/10C12N5/0775
CPCA61K35/28A61P9/10A61P27/02C12N5/0663C12N2509/00
Inventor 邵正波刘鑫娜原慧萍王姝婧程方
Owner HARBIN MEDICAL UNIVERSITY
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