Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Single cell sorting device and single cell sorting method

A single-cell and sorting technology, applied in the field of single-cell manipulation, which can solve problems such as low accuracy, affecting cell activity, and cell mechanical damage

Pending Publication Date: 2020-08-07
GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although fluorescence-activated cell sorting can be used as an effective means of separating cells, this method requires the addition of fluorescent labels to cells, which changes the original state of cells and sometimes affects cell viability; laser capture microdissection is generally used for large-area cells. Separation, low precision, difficult to achieve high throughput, and easy to cause mechanical damage to cells during cutting

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Single cell sorting device and single cell sorting method
  • Single cell sorting device and single cell sorting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 single cell sorting device

[0052] Such as figure 1 Shown is a schematic diagram of a single cell sorting device, which includes a microfluidic chip, an optical recognition unit, and an electrical sorting unit;

[0053] The microfluidic chip includes a sample inlet, a microfluidic channel and a sample outlet;

[0054] The sampling port includes a first sampling port and a second sampling port, and the first sampling port and the second sampling port are collected in a microfluidic channel;

[0055] The microfluidic channel is filled with an oil phase;

[0056] An optical recognition unit and an electrical sorting unit are sequentially arranged on the microfluidic channel;

[0057] The sample outlet is located downstream of the electrical separation unit;

[0058] The sample inlet is connected with a sample pump;

[0059] The optical recognition unit includes a camera;

[0060] The camera comprises a charge-coupled device camera or a complementary met...

Embodiment 2

[0066] Embodiment 2 single cell sorting device

[0067] The single cell sorting device includes a microfluidic chip, a charge-coupled device camera and electrodes;

[0068] The microfluidic chip includes a sample inlet, a microfluidic channel and a sample outlet;

[0069] The sample inlet includes a first sample inlet and a second sample inlet, which are located at the left end of the microfluidic chip, and are used to add cell suspension and microspheres to the microfluidic chip through an air pressure source and a sample pump. The first sample inlet and the second sample inlet are collected in the microfluidic channel;

[0070] The microfluidic channel is filled with an oil phase, placed between the optical lens objective lens and the eyepiece, and connected to a charge-coupled device camera;

[0071] Electrodes are arranged on both sides of the microfluidic channel, the voltage of the electrodes is ±100-±40000V, the width of the electrodes is 60-200μm, and the frequency o...

Embodiment 3

[0074] Example 3 single cell sorting method

[0075] Such as figure 2 Shown is the flow chart of the single-cell sorting method. Under the action of the sample pump, cell suspension and PS microspheres (particle size: 8~12μm), after the two are collected in the microfluidic channel, the cell suspension is mixed with the microspheres, and the microspheres can specifically capture single cells in the cell suspension;

[0076] After the microspheres with captured single cells are injected into the oil phase in the microfluidic channel, water-in-oil single-cell droplets containing single cells and microspheres are formed. The particle size of the single-cell droplets is 25-30 μm, and no Single-cell microspheres form water-in-oil microsphere droplets, and the particle size of the microsphere droplets is 15-20 μm;

[0077] The camera identifies single-cell droplets with larger particle sizes in the microfluidic channel, and uses image recognition algorithms to convert digital ima...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Widthaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a single cell sorting device and a single cell sorting method. The device comprises a micro-fluidic chip for generating and transporting single cell liquid drops, an optical identification unit for identifying single-cell liquid drops, and an electrical sorting unit for controlling the single-cell liquid drops. The micro-fluidic chip is used as a carrier, the optical identification unit is used for identifying single-cell liquid drops in the micro-fluidic chip, a digital image is converted into an image signal based on an image identification algorithm, the image signalis transmitted to a special image processor, and the processor reads an identification signal and sends an electric field applying signal to the electrical sorting unit; the electrical sorting unit applies a dielectric force to the single-cell liquid drops, and the single-cell liquid drops are separated from the microsphere liquid drops without the single cells. Therefore, single-cell sorting is achieved, and the single-cell sorting rate is greatly increased.

Description

technical field [0001] The invention belongs to the technical field of single cell manipulation, and relates to a single cell sorting device and a single cell sorting method. Background technique [0002] Single-cell research has achieved remarkable results in the fields of proteomics, microscopic imaging, and oncology, and has gradually developed into a key research field, and single-cell sorting technology is the focus of single-cell research. [0003] On the microfluidic chip, by applying different forces to the microfluid, such as dielectric force, magnetic force, acoustic force, optical tweezers, etc., combined with fluid power in the microfluidic channel, the manipulation of single cells can be realized. sorting. Single-cell manipulation methods based on microfluidic chips include dielectrophoresis technology, optical tweezers technology, hydrodynamic technology, magnetic field force technology and centrifugal force technology, but there are more or less low sorting e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/42C12M1/34C12M1/00C12N5/00C12N13/00B01L3/00
CPCC12M47/04C12N5/00C12N13/00B01L3/502715B01L3/50273B01L3/502761B01L2200/0652B01L2200/0668B01L2300/021B01L2300/0654B01L2400/0424
Inventor 张骁范卫华樊科骆健忠温晓慧
Owner GUANGZHOU INST OF BIOMEDICINE & HEALTH CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com