Diagnostic kit for combined diagnosis of mycoplasma pneumonia and application of diagnostic kit

Abstract

The invention relates to a diagnostic kit, in particular to the diagnostic kit for the combined diagnosis of mycoplasma pneumonia. The kit comprises a single-side detection test strip and a double-side detection test strip, a mycoplasma pneumoniae antibody marked by time-resolved fluorescent microspheres is fixed on a combination pad of the single-side detection test strip, a mycoplasma pneumoniaeantibody is coated on a first detection line, and a goat-anti-mouse IgG antibody is coated on a first quality control line. A time-resolved fluorescent microsphere labeled mouse anti-human IgM monoclonal antibody and a mouse anti-human IgG antibody are fixed on a combination pad of the double-detection test strip, a second detection line and a third detection line are both coated with natural MP-Ag, and the second quality control line is coated with a goat anti-mouse IgG antibody. According to the invention, the time-resolved fluorescent microsphere is used as a chromogenic marker, the detection test strip for detecting the mycoplasma pneumoniae IgM and IgG three times is combined into a whole, and the human mycoplasma pneumoniae antigen and the mycoplasma pneumoniae IgM and IgG are simultaneously detected, so that the kit has higher sensitivity and stronger specificity compared with a colloidal gold method.

Description

technical field [0001] The invention relates to a diagnostic kit, in particular to a diagnostic kit for combined diagnosis of mycoplasma pneumonia and its application. Background technique [0002] Mycoplasma pneumoniae is a pathogenic microorganism between viruses and bacteria. It is the smallest prokaryotic cell-type microorganism that can grow and reproduce on an inanimate medium. It can occur at any age, and its infection method can be transmitted in the form of aerosol particles. It is easy to spread in dense crowds, produces respiratory tract infections in adults and children, and causes diseases such as pneumonia and bronchitis. The clinical manifestations and chest X-ray examination of mycoplasma pneumonia are not characteristic, and the diagnosis cannot be made based on the clinical manifestations of chest X-ray alone. The diagnosis is mainly based on the detection of selected pathogens. At present, the diagnosis of mycoplasma pneumonia in China mainly relies on ser...

AI Technical Summary

Problems solved by technology

The clinical manifestations and chest X-ray examination of mycoplasma pneumonia are not characteristic, and the diagnosis cannot be made based on the clinical manifestations of chest X-ray alone. The diagnosis is mainly based on the detection of selected pathogens. At present, the diagnosis of mycoplasma pneumonia in China mainly relies on serological testing.

[0003] In view of the window period of serological testing and the influence of immune conditions on the body, as well as the sensitivity and specificity of different testing methods, the final diagnosis cannot be achieved with one testing method. Therefore, the combined diagnosis of etiology and serology is still currently advocated for the diagnosis of MP infection. Strategy

[0004] At present, the colloidal gold method is mainly used to detect Mycoplasma pneumoniae IgM or Mycoplasma pneumoniae IgG, but the colloidal gold method has low sensitivity and poor specificity

Nucleic acid PCR method has high requirements for testing personnel and high testing costs

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