Primary selection method of bergamot pear rootstocks with high apple branch blight resistance

Abstract

The invention belongs to the field of pear tree disease-resistant breeding, and particularly relates to a primary selection method of bergamot pear rootstocks with high apple branch blight resistance.The method comprises the following steps of (1) collecting seeds: collecting bergamot pear rootstock seeds with character variation in the field; (2) storing the seeds in sand; (3) performing greenhouse seedling raising; (4) performing strain preservation; (5) preparing a bacterial liquid: dipping the bacterial strain, scribing on an NA culture medium, and activating for 24-48 hours; picking a single colony in an NB culture medium, and carrying out shake culture for 24-48 hours; (6) inoculating by a needling method: preparing a bacterial solution with the concentration of 1*10<5> cfu / mL, andperforming needling inoculation at the axilla of the third leaf below the top end of the seedling; (7) primary screening of seedlings: eliminating the seedlings with disease spots exceeding 25-30% ofthe height of the seedlings; and (8) secondary screening of seedlings: preparing a bacterial solution with the concentration of 1*10<8> cfu / mL, inoculating, and eliminating the seedlings with diseasespots exceeding 25-30% of the height of the seedlings. The method is high in feasibility and reliability of screening the high-disease-resistance rootstock seedlings and can be applied and popularized.

Description

technical field [0001] The invention belongs to the technical field of pear tree disease-resistant breeding, in particular to a method for primary selection of fragrant pear stock with high resistance to apple branch blight. Background technique [0002] Korla Fragrant Pear is a national geographical indication product and has won a well-known trademark in China. Its origin is only in Bazhou and Aksu in Xinjiang, covering an area of ​​more than 800,000 mu. Since the occurrence of apple branch blight in 2017, it has spread rapidly, causing a large number of dead trees and gardens, seriously threatening the production and ecological security of fragrant pears in Xinjiang. It has become a devastating plant bacterial disease of fragrant pears. Fragrant pear and its conventional rootstock, Duli, are highly susceptible varieties. After the pathogen infects the rootstock, Duli, it quickly spreads to the entire root system, resulting in browning and necrosis of the cortical tissue o...

AI Technical Summary

Problems solved by technology

[0005] The apple branch blight that has occurred in recent years is a plant bacterial disease with strong infectivity and high dead tree rate. Since it can cause devastating damage to fragrant pear trees in a short period of time, the common disease-resistant breeding method in the prior art is adopted The efficiency is too low, it is difficult to ensure the high resistance and feasibility of breeding, it is not suitable for the breeding needs of high disease-resistant fragrant pear rootstocks, and it is impossible to realize the rapid and efficient breeding of high-resistant apple branch blight fragrant pear rootstock varieties

[0006] On the other hand, in the prior art, due to the strong infectivity and high mortality rate of the strains of apple branch blight, as well as the complex influence of its host and environment, there is currently a worldwide discussion on highly resistant apple branch blight There are few reports on rootstock breeding research