A preparation method of cell 3D printing bioink with good printability

Abstract

The invention relates to a preparation method of cell 3D printing bio-ink with good printability, which is to process the biological tissue powder with pepsin digestion time of 3 hours or less to make the bio-tissue-derived bio-ink for cell 3D printing . The toothpaste-like bio-ink prepared by this technical scheme has better printability, and the scaffold printed with it has a larger void structure, which is conducive to the contact of cells with nutrients and oxygen, which in turn is conducive to the cells to play their biological functions ; The 3D printed structure will not collapse, and the printed 30 layers are still continuous, which can be used to build a more complex three-dimensional multi-layer structure. Compared with the digestion time of the state-of-the-art bioink, the planar printing accuracy and multi-layer accumulation ability of this pure tissue-derived bioink are improved, which is beneficial to the fabrication of various bioactive tissue organoid structures, in tissue It has a wide range of applications in the fields of engineering, regenerative medicine and organ models.

Description

technical field [0001] The invention belongs to the technical field of 3D printing bio-ink, in particular to a preparation method of cell 3D printing bio-ink with good printability derived from biological tissue. Background technique [0002] 3D cell printing technology has been widely used in tissue engineering and regenerative medicine models. Using this advanced additive manufacturing technology, researchers can prepare heart tissue, lung tissue, kidney tissue, liver tissue, pancreatic tissue or muscle tissue. [0003] The preparation of bio-inks with good biological activity and printability is a key step in the realization of cell 3D printing. Some chemically synthesized materials and natural materials have been widely used in the preparation of bio-inks. For example: alginate, gelatin, collagen, cellulose, polyethylene glycol and mixtures thereof. However, the bioinks prepared by these materials cannot fully simulate the microenvironment of cells in biological tissues...

AI Technical Summary

Problems solved by technology

However, this kind of temperature-controlled temperature self-installation occurs at a slower speed, which makes the printed lines widen and collapse during the printing process, which seriously affects the printing accuracy, such as figure 1 As shown, using the tissue-derived bio-ink produced by the prior art 1 to carry out 3D printing line widening and collapse, the pore structure is blocked, which is not conducive to the contact of cells with nutrients and oxygen; as figure 2 As shown, using the tissue-derived bio-ink produced by Technology 1 for 3D printing, due to the slow self-assembly of collagen, it collapses before it is completely gelled, and it is impossible to make a biological model with a complex structure; how to improve this tissue-derived biological ink? Ink printability is a technical difficulty

[0016] The existing technology proposes that the printability of this tissue-derived bioink can be improved by mixing other types of inks. However, this will change the microenvironmental structure and biochemical composition of the ink, which is not conducive to simulating the tissue microenvironment. How to achieve this without changing its composition? Improving the printability of this bioink based on

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