Immunofluorescence kit for detecting EGFR gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient and detection method

A non-small cell lung cancer, peripheral blood circulation technology, applied in the field of molecular biology, can solve the problems of inability to obtain tissue samples, inability to assess the EGFR status of patients, inability to obtain tissue samples in real time or by repeated puncture, and to avoid edge effects and stability. Good, reducing the effect of cell loss

Pending Publication Date: 2020-08-18
SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the shortcomings of patients with advanced or recurrent non-small cell lung cancer who cannot obtain tissue samples by real-time or repeated puncture, and thus cannot evaluate the patient's EGFR status, the present invention provides a non-diagnostic detection method for EGFR gene mutations in peripheral blood circ

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunofluorescence kit for detecting EGFR gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient and detection method
  • Immunofluorescence kit for detecting EGFR gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient and detection method
  • Immunofluorescence kit for detecting EGFR gene mutation of peripheral blood circulating tumor cells of non-small cell lung cancer patient and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Use a membrane filtration device to separate and obtain CTCs in the peripheral blood of patients with advanced or recurrent non-small cell lung cancer whose tissue samples cannot be obtained, and determine whether CTCs exist:

[0047] Collect 5ml of fasting blood for 8-12 hours from the median vein of the elbow, and use 45ml of diluent (ingredients: 1mmol / L EDTA+0.1%BSA+0.1%trehalose+0.2%polyoxyethylene polyoxypropylene ether block copolymer) Dilute the peripheral blood, then add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes;

[0048] At fixed intervals, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filtering device is composed of a filter 3, a filter membrane 7, a blood sample container 2, a waste liquid tank 5, and an iron stand 1;

[0049] Wet the filter 3 with 10ml of PBS, and then add the fixed peripheral blood sample into the blood sample container 2 of the membrane filtration d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides an immunofluorescence kit for detecting EGFR gene mutation of peripheral blood circulating tumor cells of a non-small cell lung cancer patient and a detection method, and belongs to the technical field of molecular biology. The kit comprises 45 mL of a diluent, 1 mL of a decolorizing solution, 0.5 mL of a staining solution A, 1 mL of a staining solution B, 200 [mu]l of methanol, 200 [mu]l of 2% PFA, 100 [mu]l of 10% goat serum, 100 [mu]l of a primary antibody working solution, 100 [mu]l of a secondary antibody working solution and a DAPI mounting medium. According to thedetection method provided by the invention, the EGFR expression condition of a patient suffering from advanced or recurrent non-small cell lung cancer can be detected without obtaining a tissue specimen through needle biopsy. The technology belongs to minimally invasive technology and can realize real-time detection. According to the method provided by the invention, a false positive result caused by an edge effect possibly generated in the dyeing process can be avoided, the stability is good, the cell loss is reduced, and the detection accuracy is improved.

Description

technical field [0001] The invention provides an immunofluorescence kit and a detection method for detecting EGFR gene mutation in peripheral blood circulating tumor cells of patients with non-small cell lung cancer, belonging to the technical field of molecular biology. Background technique [0002] Lung cancer is one of the main malignant tumors leading to the death of cancer patients. In my country, the morbidity and mortality of lung cancer rank first. Non-small cell lung cancer (NSCLC) accounts for about 85% of all lung cancers, and more than 70% of NSCLC patients are diagnosed at an advanced stage. Despite the continuous improvement of treatment techniques such as surgery, radiotherapy and chemotherapy, the 5-year survival rate of NSCLC patients is still less than 20%, and the main causes of death include local recurrence and distant metastasis. [0003] Circulating tumor cells (CTCs) are tumor cells shed from solid tumors and enter the peripheral blood circulation. S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/58G01N33/574
CPCG01N33/57423G01N33/57492G01N33/582G01N2333/71
Inventor 王振丹李胜李浩胡敏
Owner SHANDONG RES INST OF TUMOUR PREVENTION TREATMENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap