Preparation method of bionic tissue engineering scaffold with photo-thermal responsiveness and controllable drug release
A tissue engineering scaffold and responsive technology, which is applied in the field of preparation of bionic tissue engineering scaffolds, can solve problems such as accelerating nerve regeneration, achieve the effects of improving success rate, benefiting long-term treatment and rehabilitation, and improving biological functionality
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specific Embodiment 1
[0061] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:
[0062] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 50 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the s...
specific Embodiment 2
[0070] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:
[0071] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 100 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the ...
specific Embodiment 3
[0079] The first step is to prepare a nanoparticle with photothermal effect loaded with bioactive molecules:
[0080] Take tris-tris to prepare a Tris solution with a pH of 8.5, then add Dopa powder to the Tris solution to prepare a polydopamine solution, and finally add 150 mg of nanoparticles to 10 ml of the polydopamine solution. Wrap the container containing the mixed solution with tin foil to avoid light, ventilate it, and shake it in a mechanical shaker. After shaking, remove the solution from the shaker container, discard the supernatant, take out the precipitate and dry it. Then dry the obtained powder and wash it again with Milli-Q. After centrifuging again, the supernatant was discarded, and the precipitate was dried to obtain Dopa@MWCNT powder. Add Dopa@MWCNT powder into 20ml of DFO / YR mixed solution, and shake it in a mechanical shaker. After shaking, the solution was taken out from the shaker and placed in a centrifuge for centrifugation. Finally, discard the ...
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