Preparation method, product and application of a human von Willebrand factor/human coagulation factor ⅷ complex
A technology for hemophilia factor and human coagulation factor, which is applied in the field of preparation of human von Willebrand factor/human coagulation factor VIII complex, can solve problems such as complicated steps, reduce the risk of thromboembolism, achieve high quality, The effect of improving safety
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0052] The invention provides a preparation method of a human von Willebrand factor / human blood coagulation factor VIII complex, comprising the following steps:
[0053] (1) Take the frozen plasma, centrifuge after thawing the plasma, and collect the cryoprecipitate;
[0054] (2) Dissolving the cryoprecipitate described in step (1), adjusting the pH value to 6.35-6.55, centrifuging at 12-18°C, and collecting the supernatant;
[0055] (3) Mix the supernatant described in step (2) with the aluminum hydroxide gel, centrifuge after stirring and absorbing, and collect the supernatant;
[0056] (4) Adjust the protein concentration of the supernatant in step (3) to 5.0-15.0 g / L with an equilibrium buffer, then filter in series at 0.6-0.7um+0.4-0.5um, and collect the filtrate;
[0057] (5) Mix the filtrate described in step (4) with the S / D solution, stir at 24-26° C. for 5-7 hours, and then filter, the filter tool for the filtration has a pore size of 0.4-0.5 μm, and collect the fil...
Embodiment 1
[0085] Taking 6000 liters of plasma as an example, the specific preparation process is as follows:
[0086] (1) During the quarantine period, after receiving the plasma from qualified individuals, wipe the surface of the plasma bag with 75% ethanol, rinse it with water for injection below 35°C, break the bag and merge it into a slurry tank, and circulate it with circulating water at 30-35°C. The temperature of plasma should not be higher than 4°C; after melting, it should be centrifuged (centrifugal force should not be lower than 6000g), the temperature of the liquid should be controlled at 0-4°C, and 34.8kg of cryoprecipitate should be collected;
[0087] (2) Add the cryoprecipitate obtained in step (1) into 209kg of dissolving solution (dissolving solution preparation method: 2.94g sodium citrate, 3000IU heparin sodium, add water for injection to 1L, and control the temperature at 20-26°C) , stir until the cryoprecipitate is completely dissolved, and the temperature of the c...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com