Culture method of adipose-derived stem cells and application of adipose-derived stem cells in arthritis
An adipose stem cell and culture method technology, applied in the field of adipose stem cell culture, can solve problems such as side effects and limited treatment effect of arthritis, and achieve the effect of good treatment effect
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Embodiment 1
[0043] A method for culturing fat stem cells, the specific steps are as follows:
[0044] (1) Primary culture of adipose-derived stem cells
[0045] 1) Take 5ml of adipose tissue (obtained from the subcutaneous tissue of surgical patients), and then squeeze the adipose tissue into a prepared 15ml centrifuge tube at one time, and the adipose tissue in each centrifuge tube shall not exceed 5ml;
[0046] 2) Add 7ml of buffer solution PBS into the above-mentioned centrifuge tube containing adipose tissue, pipette it 10-20 times, then let it stand for about 1min, and use the straw to suck out the liquid in the lower layer of the tissue; repeat this until the sucked out liquid is Transparent until there are no blood cells;
[0047]3) Add about 5ml of digestive solution 1 with the same volume as the tissue to the centrifuge tube with adipose tissue, seal the centrifuge tube, put it in a constant temperature shaker at 37°C, 190r / min, shake and digest for 30min; There are 3 layers, t...
Embodiment 2
[0066] A 56-year-old male suffered from grade II osteoarthritis of the right knee, and the patient's autologous adipose stem cells were cultured according to the method of Example 1, and the concentration of the adipose stem cells was 5x10 7 1 / mL, 2ml per knee joint injection, 1 injection per month, 4 injections into the articular cavity, no discomfort or discomfort after injection. After 12 months, X-rays were irradiated, and the results were as follows: figure 1 As shown, it shows that the joint space widens after 12 months after adipose stem cell treatment, indicating that the narrowing of the knee joint space caused by long-term chronic inflammation has been significantly improved, and the cartilage can be repaired.
Embodiment 3
[0068] A 45-year-old male suffers from grade II osteoarthritis cartilage defect. The patient's autologous adipose stem cells were cultured according to the method in Example 1, and the concentration of the adipose stem cells was 5×10 7 1 / mL, each knee joint was injected with 2ml each time, injected once a month, and injected into the articular cavity four times, and there was no discomfort or discomfort after the injection. After 6 months, an MRI test was performed, and the results were as follows: figure 2 As shown, the cartilage was repaired 6 months after the treatment with adipose-derived stem cells, indicating that autologous adipose-derived stem cells can repair cartilage defects.
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