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Biostable solvent for determining low-temperature fluorescence spectrum of chlamydomonas

A fluorescence spectrum, low temperature technology, used in fluorescence/phosphorescence, measurement devices, material analysis by optical means, etc., can solve the problems of unable to truly reflect the physiological and biochemical state of Chlamydomonas, poor cell stability, easy water loss and shrinkage, etc. , to avoid the consequences of poor sample permeability and strong fluorescence signal

Inactive Publication Date: 2020-09-04
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cell structure is relatively simple, and it is a model organism for studying the state transition mechanism, but the cell stability is poor, and it is easy to lose water and shrink, changing the cell shape, so it cannot truly reflect the real-time physiological and biochemical state of Chlamydomonas

Method used

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  • Biostable solvent for determining low-temperature fluorescence spectrum of chlamydomonas
  • Biostable solvent for determining low-temperature fluorescence spectrum of chlamydomonas

Examples

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example 77

[0021] figure 1 It is the example 77K low-temperature fluorescence spectrogram that adopts the method of the present invention to measure;

[0022] figure 2 It is the 77K low-temperature fluorescence spectrum diagram of the comparative example.

example 1

[0023] Example 1 Chlamydomonas Low Temperature Fluorescence Spectrum Sample Preparation

[0024] 1. Raw material composition

[0025] 1.4 parts of N-(hydroxymethyl)methylglycine, 1 part of trishydroxymethylaminomethane, 50 parts of glycerol, 0.6 parts of sodium hydroxide, and 45 parts of water.

[0026] 2. Preparation method

[0027] (1) Take an appropriate amount of N-(hydroxymethyl)methylglycine and trishydroxymethylaminomethane, add distilled water and stir to dissolve;

[0028] (2) Add glycerol to the mixed solution prepared in step (1) and stir evenly;

[0029] (3) Add sodium hydroxide to the mixed solution prepared in step (2) to adjust the pH value to 7.0.

[0030] 3. Chlamydomonas sample preparation

[0031] Take an appropriate amount of Chlamydomonas liquid, add 10 times the volume of biological buffer to a non-fluorescence-absorbing glass tube, and mix well.

[0032] 4. Sample determination

[0033] Put the Chlamydomonas sample into a 77k low-temperature fluore...

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Abstract

The invention relates to a biological buffer reagent for determining fluorescence spectrum of chlamydomonas. The biological buffer reagent is prepared from the following raw materials in parts by weight: 1-2 parts of N-(hydroxymethyl) methylglycine, 0.5-1.5 parts of trihydroxymethyl aminomethane, 40-60 parts of glycerol, 0.5-1 part of sodium hydroxide and 40-50 parts of water. The chlamydomonas cell morphology can be kept, the physiological state of chlamydomonas is kept unchanged, the consequence of poor permeability of a sample caused by low-temperature freezing solidification expansion of achlamydomonas culture solution is avoided, and a fluorescence signal is strong.

Description

technical field [0001] The invention relates to a biostable solvent for measuring Chlamydomonas low-temperature fluorescence spectrum. Background technique [0002] Chloroplast is an important place for green plants to carry out photosynthesis. In chloroplast, light energy is absorbed, transmitted, and finally converted into chemical energy. This process requires photosystem II (PSII), cytochrome b6f, [0003] The four supramolecular complexes of photosystem I (PSI) and ATP synthase act synergistically. The smooth progress of the linear electron transfer process in photosynthesis requires the coordination of the electron transfer rates of the two photosystems. However, due to the difference in the absorption characteristics of PSI and PSII and the natural variation of the light quality of sunlight reaching the photosynthetic apparatus at different times of the day, the light energy absorption and excitation of the two photosystems are often unbalanced. In order for plants ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/635
Inventor 于倩倩
Owner UNIV OF JINAN
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