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Biological preparation for treating diarrhea of pets

A biological agent and diarrhea technology, applied in the field of veterinary medicine, can solve problems such as drug residues and bacterial drug resistance, achieve inhibition of Escherichia coli and Staphylococcus aureus, reduce diarrhea frequency and loose stool rate, and inhibit canine parvovirus Effect

Active Publication Date: 2020-09-08
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main treatment for pet diarrhea is oral antibiotics. Long-term use of antibiotics can easily lead to bacterial resistance and drug residues

Method used

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  • Biological preparation for treating diarrhea of pets
  • Biological preparation for treating diarrhea of pets
  • Biological preparation for treating diarrhea of pets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Wash 500g of dried cicadae, dry it in a dryer, and crush it with a grinder;

[0046] (2) Add 10L of water and ultrasonically break for 120min;

[0047] (3) Centrifuge at 3000rpm / min for 30min, remove the precipitate, keep the supernatant, and obtain the crude polysaccharide extract;

[0048] (4) Evaporate the crude polysaccharide extract at 70°C under reduced pressure to 1L to obtain the crude polysaccharide concentrate;

[0049] (5) Adjust the crude polysaccharide concentrate to 37°C, the pH value is 8, add 100g trypsin, and enzymatically hydrolyze for 1.5h;

[0050] (6) Adjust the temperature to 95°C, inactivate for 20 minutes, add 3 times the amount of 85% ethanol, and overnight at 4°C;

[0051] (7) Centrifuge at 3000rpm / min for 30min, remove the supernatant, and freeze-dry the precipitate to obtain Cicada polysaccharide.

Embodiment 2

[0053] (1) Wash 500g of dried motherwort, dry it in a dryer, and crush it with a grinder;

[0054] (2) Add 10L of water and ultrasonically break for 120min;

[0055] (3) Centrifuge at 3000rpm / min for 30min, remove the precipitate, keep the supernatant, and obtain the crude polysaccharide extract;

[0056] (4) Evaporate the crude polysaccharide extract at 70°C under reduced pressure to 1L to obtain the crude polysaccharide concentrate;

[0057] (5) Adjust the crude polysaccharide concentrate to 37°C, the pH value is 8, add 100g of trypsin, and enzymatically hydrolyze for 1.5h;

[0058] (6) Adjust the temperature to 95°C, inactivate for 20 minutes, add 3 times the amount of 85% ethanol, and overnight at 4°C;

[0059] (7) Centrifuge at 3000rpm / min for 30min, remove the supernatant, precipitate and freeze-dry to obtain Motherwort polysaccharide.

Embodiment 3

[0061] (1) Clean 500g of chrysanthemum starfish, dry it in a dryer, place it in a grinder, add liquid nitrogen and grind it into powder to obtain chrysanthemum starfish powder;

[0062] (2) Add the chrysanthemum star powder to 1.5kg of water, stir until completely dissolved, and obtain a homogenate of chrysanthemum starfish;

[0063] (3) Adjust the temperature of Chrysanthemum starfish homogenate to 50°C, pH to 6, add 100g of papain, and enzymatically hydrolyze for 1.5h;

[0064] (4) Adjust the temperature to 95°C, inactivate for 20 minutes, cool to room temperature, centrifuge at 3500 rpm / min for 30 minutes, collect the supernatant, and obtain the crude polypeptide extract;

[0065] (5) After passing the crude polypeptide through a nanofiltration membrane with a pore size of 2 nm, freeze-drying to obtain the chrysanthemum starfish polypeptide.

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Abstract

The invention provides a biological preparation for treating diarrhea of pets, and belongs to the technical field of animal medicine. The biological preparation provided by the invention is prepared from isaria cicadae miq polysaccharide, leonurus polysaccharide, biscuit sea star polypeptide, anthocidaris crassispina polypeptide and water. The biological preparation provided by the invention can be used for effectively inhibiting escherichia coli and staphylococcus aureus, inhibiting canine parvovirus and reducing diarrhea frequence and loose stool rate, so that pet diarrhea caused by variousdifferent reasons can be effectively treated.

Description

technical field [0001] The invention belongs to the technical field of animal medicine, in particular to a biological agent for treating pet diarrhea. Background technique [0002] With the continuous improvement of people's living standards, the number of pets is gradually increasing, and people pay more and more attention to pets. In the process of raising pets, if the pets are not well cared for, it is easy to cause pets to get sick. Among the diseases that pets suffer from, the proportion of diarrhea-related diseases is the largest among all diseases. [0003] Diarrhea refers to a pathological process in which the frequency of defecation increases, the water content of feces and the volume of feces increase, and the feces appear loose or contain more fat, mucus and blood. Pet feces are a common disease in pet clinics. . According to different causes, pet diarrhea can usually be divided into diarrhea caused by viral infection, diarrhea caused by bacteria, diarrhea caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/01A61P1/12A61P31/04A61P31/12C08B37/00C12P21/06C07K1/34A61K31/715
CPCA61K38/012A61P1/12A61P31/04A61P31/12C08B37/0003C12P21/06C07K1/34A61K31/715A61K2300/00Y02A50/30
Inventor 秦志华王晓雪郭沛王述柏
Owner QINGDAO AGRI UNIV
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