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Bacteroides fragilis capable of regulating and controlling relative abundance of akkermansia in intestinal tracts

A technology of Bacteroides fragilis and microbial strains, applied in the field of microorganisms, can solve problems such as allergic reactions and patient vomiting, achieve high safety, reduce mucosal bleeding, and reduce intestinal inflammatory cell infiltration.

Active Publication Date: 2020-09-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these drugs often cause side effects such as vomiting, diarrhea and allergic reactions in patients

Method used

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  • Bacteroides fragilis capable of regulating and controlling relative abundance of akkermansia in intestinal tracts
  • Bacteroides fragilis capable of regulating and controlling relative abundance of akkermansia in intestinal tracts
  • Bacteroides fragilis capable of regulating and controlling relative abundance of akkermansia in intestinal tracts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1: Acquisition of Bacteroides fragilis

[0075] Specific steps are as follows:

[0076] Taking the feces of a 4-year-old child from Guangxi, China as a sample, draw 0.5mL of the sample and add it to 5mL of BHI liquid medium, and incubate at 37°C for 18-24h to obtain the enriched sample; draw 0.5mL of the enriched The pooled sample was added to 4.5mL sterile saline to obtain 10 -1 Diluent, then draw 0.5mL10 -1 Diluted solution in 4.5mL normal saline to obtain 10 -2 Diluent, according to this operation, successively get 10 -3 , 10 -4 , 10 -5 , 10 -6 Dilution; draw 100 μL of gradient dilution and spread it on BHI solid medium, 10 -4 , 10 -5 , 10 -6 1 plate for each gradient, cultivated at 37°C for 48 hours to obtain colonies; select colonies with typical characteristics of Bacteroides fragilis on BHI solid medium according to the shape, size, edge, and transparency of the colonies, and use an inoculation loop to pick colonies on BHI solid culture Streak o...

Embodiment 2

[0083] Example 2: Effects of Bacteroides fragilis CCFM1123 on the intestinal flora composition of inflammatory mice

[0084] Specific steps are as follows:

[0085] Forty-eight healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, 12 mice in each group. The 4 groups were: the model-making blank group and the group fed with B. fragilis CCFM1123, FAHBZ17K2, and FFJLY17K5 bacterial suspensions CCFM1123, FAHBZ17K2, FFJLY17K5 intervention group.

[0086] The experimental process is as follows: the mice in the model-making blank group were given 200 g / L glycerol solution (containing 1 g / L cysteine ​​hydrochloride) once a day at a dose of 0.1 mL, and the mice in the CCFM1123, FAHBZ17K2, and FFJLY17K5 intervention groups According to the dosage of 0.1mL, the bacterial suspension was intragastrically administered once a day for 7 days; on the 8th day of the experiment, the mice in the modeling blank group and the CCFM1123, FAHBZ17K2, FFJLY17K5 intervention groups...

Embodiment 3

[0092] Example 3: Effects of Bacteroides fragilis CCFM1123 on the content of pro-inflammatory factors TNF-α and IL-6 in the serum of inflammatory mice

[0093]Specific steps are as follows:

[0094] Forty-eight healthy female C57 mice aged 6-8 weeks were randomly divided into 4 groups, 12 mice in each group. The 4 groups were: the model-making blank group and the group fed with B. fragilis CCFM1123, FAHBZ17K2, and FFJLY17K5 bacterial suspensions CCFM1123, FAHBZ17K2, FFJLY17K5 intervention group.

[0095] The experimental process is as follows: the mice in the model-making blank group were given 200 g / L glycerol solution (containing 1 g / L cysteine ​​hydrochloride) once a day at a dose of 0.1 mL, and the mice in the CCFM1123, FAHBZ17K2, and FFJLY17K5 intervention groups According to the dosage of 0.1mL, the bacterial suspension was intragastrically administered once a day for 7 days; on the 8th day of the experiment, the mice in the modeling blank group and the CCFM1123, FAHBZ1...

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Abstract

The invention discloses bacteroides fragilis capable of regulating and controlling relative abundance of akkermansia in intestinal tracts, and belongs to the technical field of microorganisms. The bacteroides fragilis CCFM1123 is screened out, and the bacteroides fragilis CCFM1123 has the effect of relieving inflammation. Specifically, the relative abundance of lactobacillus and akkermansia in theintestinal tract of an inflammatory mouse is remarkably improved; the content of proinflammatory factors TNF-alpha and IL-6 in the serum of the inflammatory mouse is remarkably reduced; intestinal inflammatory cell infiltration and mucosal bleeding caused by inflammatory response of the inflammatory mouse are remarkably reduced; and production of short-chain fatty acids in the intestinal tract ofthe inflammatory mice is obviously promoted. Therefore, the bacteroides fragilis CCFM1123 has a huge application prospect in preparation of products (such as foods or medicines) for preventing and / ortreating inflammation.

Description

technical field [0001] The invention relates to a strain of Bacteroides fragilis capable of regulating the relative abundance of the genus Akkermansia in the intestinal tract, and belongs to the technical field of microbes. Background technique [0002] Inflammation is a common clinical pathological process that can occur in tissues and organs in various parts of the body, such as folliculitis, tonsillitis, pneumonia, hepatitis, nephritis, etc. Acute inflammation usually has changes such as redness, swelling, heat, pain, and dysfunction, and is often accompanied by systemic reactions such as cascade effect of inflammatory factors, intestinal microbial imbalance, fever, and leukocytosis. [0003] Among them, the cascade effect of inflammatory factors is manifested in the secretion of a large number of cytokines in a short period of time, resulting in serious damage to human tissues and organs. Once the cytokine cascade effect occurs, it will bring various complications such ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L2/52A61K35/741A61P31/00A61P29/00A61P37/02A61P3/04C12R1/01
CPCA23L33/135A23L2/52A61K35/741A61P31/00A61P29/00A61P37/02A61P3/04A23V2002/00C12R2001/01C12N1/205A23V2200/30
Inventor 翟齐啸陈卫孙凤婷田丰伟于雷雷陆文伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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