Construction method and application of heat-resistant yeast engineering strain producing xylitol under high-temperature aerobic condition

A technology of engineering strains and heat-resistant yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve problems such as ethanol accumulation, low ratio, unfavorable product purification, etc.
CN111662832AActive Publication Date: 2020-09-15HUAIBEI NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAIBEI NORMAL UNIVERSITY
Publication Date
2020-09-15

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Abstract

The invention discloses a heat-resistant yeast engineering strain producing xylitol under a high-temperature aerobic condition and a construction method and application thereof. The heat-resistant yeast engineering strain is a heat-resistant yeast engineering strain YZB194, and the classification name of the strain is Kluyveromyces marxianus, the strain is collected in the China General Microbiological Culture Collection Center (CGMCC) in No.3 Yard 1, Beichen West Road, Chaoyang District, Beijing on November 25, 2019 with the collection number of CGMCCC NO.19004. YZB194 synthesizes 203g / L xylitol by using 121 g / L glucose and 205 g / L xylose by batched supplement fermenyation with a fermentation tank under the aerobic condition of 42 DEG C, the synthesis rate of xylitol is 2.99g / L / h, and theconversion rate of xylitol reaches 99% of the maximum theoretical value.
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Description

technical field

[0001] The invention belongs to the field of microbial metabolism engineering and microbial fermentation engineering, and specifically relates to a heat-resistant yeast engineering strain producing xylitol under high-temperature aerobic conditions, a construction method and application thereof. Background technique

[0002] Xylitol is a five-carbon polyol that is a normal intermediate in xylose metabolism

[11] . When it is used by the human body, it does not need the promotion of insulin, and it can promote the secretion of a small amount of insulin in the body; when it is used for intravenous infusion, the content of pyruvic acid, lactic acid and glucose in the blood will decrease, and the liver glycogen will increase. ; Non-cytotoxic, can pass through the cell membrane and become the nutrition of the organization. These characteristics make xylitol have important application value. In addition, as a sweetener, xylitol has a cool taste and can prevent denta...

Claims

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