Sulfadimidine aptamer screening method, kit and application

A technology of sulfamethazine nucleic acid and sulfamethazine, applied in biochemical equipment and methods, biological testing, material inspection products, etc., can solve the problems of long experimental period, expensive chromatography, cumbersome processing process, etc., and achieve screening The number of rounds is short, the detection operation is simple, and the target is not fixed.
CN111662900AActive Publication Date: 2020-09-15CHONGQING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHONGQING NORMAL UNIVERSITY
Publication Date
2020-09-15

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Abstract

The invention provides a sulfadimidine nucleic acid aptamer screening method and a sulfadimidine nucleic acid aptamer detection kit. The kit comprises a fluorescence aptamer sensor based on graphene oxide, wherein the fluorescence aptamer sensor based on graphene oxide comprises an aptamer marked by an FAM fluorophore and graphene oxide, wherein the sequence of the sulfadimidine aptamer marked bythe FAM fluorophore is No 1s recorded in a nucleotide sequence table. The invention discloses an application of the sulfadimidine fluorescence detection kit in quantitative detection of sulfadimidinein test samples of milk or eggs.
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Description

technical field

[0001] The invention relates to the fields of biotechnology and food antibiotic residue detection. More specifically, it relates to a sulfamethazine nucleic acid aptamer screening method, kit and application. Background technique

[0002] Sulfamethazine, the chemical name is 2-(p-aminobenzenesulfonamido)-4,6-dimethylpyrimidine, which is a common sulfonamide drug and a broad-spectrum antibacterial agent. In addition to being used in clinical treatment, It can also be used as a veterinary drug and feed additive, and is widely used in the feeding of food-derived animals. After entering the animal body through various administration routes, it can be transferred to animal-derived foods such as meat, eggs, and milk. If used improperly, it is easy to produce residues in animal tissues and animal-derived foods, enter the human body through the food chain, and cause harm to human health. And it is discharged into the external environment through animal feces and ot...

Claims

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