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Method for evaluating heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract

A technology for polysaccharide extraction and reproductive toxicity, which is applied in biochemical equipment and methods, pharmaceutical formulations, microbial measurement/inspection, etc., can solve the problem of low reproductive protection effect, achieve short test period, low cost, and simple evaluation method Effect

Active Publication Date: 2020-09-15
UNIV OF SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still relatively few studies on the reproductive protection effect of polysaccharides. It will be of great significance for its pharmacological research to use it to resist reproductive toxicity caused by heavy metal exposure.
[0004] At present, there is no article that proposes to use Caenorhabditis elegans as a research model to evaluate the anti-heavy metal reproductive toxicity of traditional Chinese medicine polysaccharide extracts

Method used

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  • Method for evaluating heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract
  • Method for evaluating heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract
  • Method for evaluating heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract

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Embodiment 1

[0085] The cultivation and exposure of nematodes were carried out as follows:

[0086]In this embodiment case, the cultivation of nematodes can be a method well known to those skilled in the art, and the present invention is preferably: the wild-type strain Caenorhabditis elegans (N2) is grown with nematodes containing Escherichia coli (E.coli OP50) bacterium The culture medium (NGM) was cultured, and the temperature was set at 20°C. In order to obtain nematodes with approximately the same growth stage, lyse the nematodes with fertilized eggs with nematode lysis solution (containing 0.5M NaOH and 20% NaClO) to obtain the eggs in the body, and incubate the eggs in the buffer solution at 20°C for 16-24h , to obtain the L1 stage nematodes required for the experiment. In order to simulate the conservation effect of kudzu root as a food-based health product, L1 stage nematodes were exposed to a buffer solution containing 0 mg / L-200 mg / L PPS, and Escherichia coli was added as food ...

Embodiment 2

[0088] The method for measuring the number of offspring of nematodes in this implementation case: transfer the L4 nematodes to an agar plate with food to allow them to grow normally, and place one nematode per plate. The next day, count the offspring of nematodes under a microscope, including unhatched eggs and hatched larvae, and the parental adults are then transferred to fresh food plates. Repeat the operation of transferring plates and counting the number of offspring until the nematodes no longer produce offspring. 15 nematodes per group.

[0089] Attached from the manual figure 1 It can be seen from the results in that there is a significant difference between the total offspring number of Pueraria polysaccharides (50-200mg / L) and cadmium co-exposure group and the total offspring number of the control group (the symbol * represents a significant difference compared with the control group, P<0.05), the total progeny number of nematodes does not belong to the open interv...

Embodiment 3

[0091] The method for measuring the number of eggs conceived by nematodes in this example: pick a single nematode and place it in the droplet of lysate, after a few minutes the parent body of the nematode is lysed, leaving only undischarged eggs on the plate. The number of fecundated eggs of each nematode in each group was photographed and recorded, and 15 nematodes were tested in each group.

[0092] Attached from the manual figure 2 As can be seen from the results in the figure, there is a significant difference between the number of fertilized eggs in the pueraria polysaccharide (150mg / L) and cadmium co-exposure group and the control group (the symbol * represents a significant difference compared with the control group, P< 0.05), the number of fertile eggs of nematodes does not belong to the open interval formed by the number of fertile eggs of the blank group and the control group, and the total number of offspring is closer to the index value of the blank group. The re...

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Abstract

The invention provides a method for evaluating the heavy metal reproductive toxicity resistance effect of traditional Chinese medicine polysaccharide extract. The method comprises the following stepsof exposing caenorhabditis elegans to culture mediums of a blank group, a control group and a tested group, and evaluating the heavy metal reproductive toxicity resistance effect of the traditional Chinese medicine polysaccharide extract according to the total generation number, the egg number, the germ cell apoptosis number and the progeny growth and reproduction conditions of the caenorhabditiselegans, wherein the culture medium of the blank group comprises a buffer solution and escherichia coli; the culture medium of the control group comprises a buffer solution, escherichia coli and heavymetal ions, and the culture medium of the tested group comprises a buffer solution, escherichia coli, traditional Chinese medicine polysaccharide extract and heavy metal ions. The method for evaluating the heavy metal reproductive toxicity resistance effect of the traditional Chinese medicine polysaccharide extract provided by the invention is simple, low in cost and short in test period; and meanwhile, technical support is provided for reproduction protection research of traditional Chinese medicine polysaccharide products, and the heavy metal reproductive toxicity resistance effect of polysaccharide and other health-care foods or drugs can be evaluated.

Description

technical field [0001] The invention relates to the technical field of efficacy detection, in particular to an evaluation method for the anti-reproductive toxicity effect of heavy metal polysaccharide extracts of traditional Chinese medicines. Background technique [0002] Heavy metals are recognized as a class of environmental pollutants, causing toxicity to organisms including the digestive system, nervous system, respiratory system and reproductive system. In recent years, the heritability of the toxicity of heavy metals or other pollutants has also been reported, indicating that the research on the threat of poisons has expanded from simple individuals to generations. Therefore, eliminating and blocking the damage caused by heavy metals in the organism is of great significance not only to the parents but also to the offspring. [0003] my country's traditional Chinese medicine resources are well-known in the world. Many traditional Chinese medicines with the same origi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N21/64A61K49/00
CPCG01N33/5014G01N33/5044G01N21/6458A61K49/0008Y02A50/30
Inventor 童中华赖杨丽邵新月
Owner UNIV OF SCI & TECH OF CHINA
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