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Kit for detecting nucleoside antiviral drugs in serum and application of kit

An antiviral drug and kit technology, which is applied in the field of kits for detecting nucleoside antiviral drugs in serum, can solve the problems of long analysis time for a single sample, large sample size, unsuitable linear range, etc., and achieves reliable detection methods, The effect of low cost and simple pretreatment process

Pending Publication Date: 2020-09-15
NANJING PINSHENG MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting the concentration of antiviral drugs in the body mainly include high performance liquid chromatography and ultra-high performance liquid phase-tandem mass spectrometry. For example, patent CN 109900819 A discloses a combination of UPLC / MS-MS for the detection of tenofo in human serum. Wei's method, patent CN 109239215 A discloses a method for the determination of entecavir in serum or plasma by isotope dilution ultra-high performance liquid chromatography mass spectrometry, but these methods require a large sample size and only detect one drug, and there is a single sample analysis time Long, unsuitable linear range and other deficiencies

Method used

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  • Kit for detecting nucleoside antiviral drugs in serum and application of kit
  • Kit for detecting nucleoside antiviral drugs in serum and application of kit
  • Kit for detecting nucleoside antiviral drugs in serum and application of kit

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Experimental program
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Embodiment 1

[0137] 1. Experimental materials and instruments

[0138] The samples for the kit research experiment came from serum samples collected from the outpatient clinic of Nanjing Drum Tower Hospital in August 2019.

[0139] 1. Instruments: Xevo TQ-S triple quadrupole mass spectrometer (Waters Corporation); UPLC I-Class ultra-high performance liquid chromatography system (with automatic sampler, Waters Corporation); SCILOGEX D2012 high-speed desktop centrifuge (USA); Ultrapure water instrument (ELGALabWater, UK); multi-tube vortex mixer (Vortex genie2, US); adjustable pipette (Eppendorf 0.5-10 μL, 10-100 μL, 100-1000 μL); glassware, graduated cylinder, etc.

[0140] 2. Reagent consumables: MS grade methanol (Fisher, USA); MS grade acetonitrile (Fisher, USA); HPLC grade acetonitrile (Honeywell, USA); MS grade formic acid (Fisher, USA); HPLC grade methanol (Honeywell, USA); Chromatographic column ACQUITY UPLC BEH C18 (2.1×50mm, 1.7μm).

[0141] 3. Standards: Standards and their corr...

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Abstract

The invention discloses a kit for detecting nucleoside antiviral drugs in serum and an application of the kit, and belongs to the technical field of drug analysis. The kit provided in the invention isadopted; three common antiviral drugs, namely ganciclovir, tenofovir and entecavir, can be detected at one time; a pretreatment process is simple, the kit is low in cost, high in sensitivity and strong in specificity, separation and detection of the nucleoside antiviral drugs are completed within 3.5 min, and the accuracy and precision basically meet requirements; and the kit can be used for quantitative analysis of the nucleoside antiviral drugs clinically, and a reliable detection method is provided for monitoring a treatment concentration of the nucleoside antiviral drugs clinically.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, and in particular relates to a kit for detecting nucleoside antiviral drugs in serum and an application thereof. Background technique [0002] Nucleotide antiviral drugs are the first choice for the clinical treatment of viral diseases such as AIDS and hepatitis, and their targets are to inhibit the DNA polymerase of DNA viruses or the reverse transcriptase of RNA viruses. Tenofovir (TNF) is a new type of nucleoside reverse transcriptase inhibitor, and it is the first-line antiviral drug for the treatment of HIV and hepatitis B virus infection. advantages, but its bioavailability is poor, and its concentration needs to be monitored in real time. Ganciclovir (GCC) is also a nucleotide analogue. Oral administration of Ganciclovir can be used to treat infections such as hepatitis virus, human cytomegalovirus (CMV), and herpes simplex virus (HSV). Entecavir (Entecavir, ETC) is a high-efficien...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/72
CPCG01N30/02G01N30/06G01N30/54G01N30/72G01N2030/027G01N2030/062
Inventor 成晓亮李美娟
Owner NANJING PINSHENG MEDICAL TECH CO LTD