Lysosome labeled magnetic fluorescent starch nanoparticles and preparation method thereof

A technology of starch nano and lysosomes, applied in the field of materials, can solve the problems of no luminescent groups, labeling and separation difficulties, etc., and achieve the effect of large phagocytosis, easy enrichment and separation, and high degree of substitution

Active Publication Date: 2020-09-15
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The surface of nano-starch granules is easy to be functionalized, and can be combined with antigens, antibodies and various proteins, so it has broad market prospects in cell separation, targeted drugs, immunoassays, etc., but nano-starch granules themselves have no luminescent groups. Recently, scientific researchers combined biodegradable nanoparticles with fluorescent molecules to make fluorescent nanoparticles, which enhanced the photochemical stability and biocompatibility of fluorescent molecules, reduced cytotoxicity and photobleaching, and further expanded the use of fluorescent labels in Research on live tracking, but labeling and separation in fluorescent labeling technology is always a difficult research point

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  • Lysosome labeled magnetic fluorescent starch nanoparticles and preparation method thereof
  • Lysosome labeled magnetic fluorescent starch nanoparticles and preparation method thereof
  • Lysosome labeled magnetic fluorescent starch nanoparticles and preparation method thereof

Examples

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Embodiment 1

[0026] This embodiment includes the following steps:

[0027] (1) Add 4.5mL 2% soluble starch to 50mL containing 0.03MFe 3+ in aqueous solution at 90 o Keep stirring at C temperature for 30min to disperse the two evenly; then slowly add the mixture solution dropwise to 30mL of 5M NH 3 ·H 2 O solution, keep the pH value of the solution at 10, at 60 o Keep stirring at C temperature for 30min, precipitate, centrifuge, wash and dry to obtain Fe with an average particle size of 200nm 3 o 4 Magnetic starch nanoparticles;

[0028] (2) Weigh 1 mg of magnetic starch nanoparticles and disperse in 1 mL of phosphate buffered saline (PBS), shake well, and pour into poly-lysine solution (poly-lysine / PBS, 0.1 mg / mL), React at 40°C for 2 h, shake on a 400r / min shaker. After the reaction, centrifuge, discard the supernatant, wash the precipitate with distilled water several times, and freeze-dry to obtain polylysine starch nanometer rich in amino groups. particles;

[0029] (3) Dispers...

Embodiment 2

[0036] This embodiment includes the following steps:

[0037] (1) Add 4.5mL 2% soluble starch to 50mL 0.03M Fe 3+ in aqueous solution at 90 o Keep stirring at C temperature for 30min to disperse the two evenly; then slowly add the mixture solution dropwise to 30mL 5MNH 3 ·H 2 O solution, keep the pH value of the solution at 10, at 60 o Keep stirring at C temperature for 30min, precipitate, centrifuge, wash and dry to obtain Fe with an average particle size of 200nm 3 o 4 Magnetic starch nanoparticles;

[0038] (2) Weigh 1 mg of magnetic starch nanoparticles and disperse in 1 mL of phosphate buffered saline (PBS), shake well, and pour into polylysine solution (polylysine / PBS, 0.1 mg / ml), React at 40°C for 3 h, shake on a 400r / min shaker. After the reaction, centrifuge, discard the supernatant, wash the precipitate with distilled water several times, and freeze-dry to obtain amino-rich polylysine starch nanoparticles. particles;

[0039] (3) Disperse 1 mg of poly-lysine ...

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Abstract

The invention discloses lysosome labeled magnetic fluorescent starch nanoparticles and a preparation method thereof. The method comprises the following steps: (1) adding a soluble starch solution intoa Fe <3+> aqueous solution, heating the solution to 80-90 DEG C, and uniformly dispersing to obtain a mixture solution; dropwise adding the mixture solution into an ammonia water solution under a stirring condition, keeping the pH value at 9-11, conducting stirring to react at 60 DEG C, separating out solids, and conducting drying to obtain magnetic starch nanoparticles; (2) electrostatically combining the magnetic nano-starch particles with lysine to obtain amino-rich poly-amino acid starch nano-particles; and (3) mixing the polyamino acid starch nanoparticles with fluorescein isothiocyanateto obtain fluorescein isothiocyanate labeled lysosome magnetic fluorescent starch nanoparticles. In the preparation process of the preparation method, one-step marking and separation of the magneticfluorescent starch nanoparticles are easy to realize. The magnetic fluorescent starch nanoparticles have the characteristics of magnetic separation and low cytotoxicity.

Description

technical field [0001] The invention belongs to the field of materials, and in particular relates to a lysosome-marked magnetic fluorescent starch nanoparticle and a preparation method thereof. Background technique [0002] Nanotechnology is a high-tech developed rapidly at the end of the last century, including the microcosm (atoms, molecules), the transition zone between microcosm and macrocosm (nano-field) and the macrocosm, and is the result of bold innovations based on traditional technologies. The first batch of research results have aroused great repercussions in the field of academic research due to their novel and unique ideas, and have received widespread attention. They are called another industrial revolution in the 21st century. With the development of the times, high-precision monitoring instruments are constantly being introduced, and nanotechnology has been further matured and perfected. As a typical representative in the field of nanotechnology, nanoparticl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N21/64C09K11/06C09K11/02
CPCG01N33/587G01N33/582G01N21/6458C09K11/025C09K11/06C09K2211/1088
Inventor 肖华西杨帆林亲录刘高强张琳许东丁玉琴文茜郑湘明
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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