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Application of carnosic acid in preparation of anti-photoaging product

A carnosic acid, anti-photoaging technology, applied in the field of medicine, to achieve the effect of natural materials and broad market prospects

Inactive Publication Date: 2020-09-22
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no research has revealed the anti-photoaging effect of carnosic acid, and the development of new anti-photoaging natural products is of great significance

Method used

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  • Application of carnosic acid in preparation of anti-photoaging product
  • Application of carnosic acid in preparation of anti-photoaging product
  • Application of carnosic acid in preparation of anti-photoaging product

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0031] Experimental Example 1: Effect of carnosic acid on the cell activity of human skin fibroblasts:

[0032] Prepare carnosic acid solutions (0.001, 0.01, 0.1, 0.5, 1, 5, 10 μM) containing the following different concentrations, and use the MTT method to detect the effect of carnosic acid (CA) on the cell viability of human skin fibroblasts, such as figure 1 shown. figure 1 Each data in the table is represented by mean ± standard error, n=3. Compared with the blank control group (control), # P## P### P<0.001; compared with the light group (UVA), *P<0.05, **P<0.01, ***P<0.001.

[0033] figure 1 The results show that when the concentration of carnosic acid is below 0.5 μmol / L, it will not inhibit the activity of human skin fibroblasts; when the concentration of CA is 1 μmol / L and 5 μmol / L, it can affect the activity of human skin fibroblasts , compared with the control group, there was a statistical difference (P<0.001); when the concentration of carnosic acid was 10 μM, ...

Embodiment 2

[0034] Example 2 Effect of carnosic acid on the cell activity of human skin fibroblasts after UVA irradiation:

[0035] 1. Prepare carnosic acid solutions (0.001, 0.005, 0.01, 0.05, 0.1, 0.5μM) containing the following different concentrations, and use UVA (10J / cm 2 *3d) Human skin fibroblasts were irradiated, and the above-mentioned different concentrations of carnosic acid solutions were added to treat for 2 hours before and after each irradiation. After the treatment, the culture was continued for 24 hours, and the effect of carnosic acid (CA) on human skin fibroblasts was detected by MTT method. The effect of the cell activity, the results obtained as figure 2 shown.

[0036] From figure 2The results showed that when the concentration of carnosic acid was 0.1-0.5 μM, it had a certain inhibitory effect on the proliferation of human skin fibroblasts, and compared with the control group, there was a statistical difference (P<0.05); when the concentration of carnosic acid ...

Embodiment 3

[0039] Anti-photoaging pharmacological research experiment of embodiment 3 carnosic acid:

[0040] 1. Carnosic acid inhibits UVA-induced β-galactosidase activity in human skin fibroblasts: in UVA (10J / cm 2 *3d) Human skin fibroblasts were treated with different concentrations of CA (0.5, 1, 2nM) before and after irradiation, and the β-galactosidase activity was detected with a β-galactosidase staining kit.

[0041] Figure 4 Carnosic acid inhibits UVA-induced cell staining positive rate of β-galactosidase activity in human skin fibroblasts, where the abscissa is the CA concentration of the cells treated with CA, and UVA(-) is UVA-irradiated , UVA (+) means after UVA radiation, and the ordinate indicates β-galactosidase staining. From Figure 4 The results showed that after UVA irradiation, compared with the control group, the positive rate of cell staining was significantly higher, with a statistical difference (P<0.001), suggesting that the aging model of fibroblasts irrad...

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Abstract

The invention discloses an application of carnosic acid in preparation of an anti-photoaging product. The product contains low-concentration carnosic acid and at least one of pharmaceutically acceptable salt and solvate thereof; the concentration of the carnosic acid is 0.01-500 nmol / L; the product includes a drug and a skin care product; the skin care product is toner or face cream; under the specific low concentration, the carnosic acid can generate the anti-aging effect, so that the damage of UVA radiation to skin tissues is effectively inhibited; and the carnosic acid has the characteristics of natural raw materials, safety and no irritation, can be widely used in the anti-photoaging drug and skin care product, and especially has wide market prospects as a new skin care variety for development.

Description

technical field [0001] The invention relates to the field of medicine, in particular to the application of carnosic acid in the preparation of anti-photoaging products. Background technique [0002] Photoaging refers to skin aging caused by long-term and repeated ultraviolet radiation on the skin. The clinical manifestations are fine wrinkles, dry skin, roughness, relaxation and pigmentation, etc., and it is easy to cause various skin cancers, which is seriously harmful. Therefore, seeking natural active compounds from plants that can prevent and treat skin photoaging has become a research hotspot in the field of skin. [0003] Most of the anti-photoaging natural products are phenolic compounds, mainly including phenolic acids, flavonoids and polymer polyphenols. The long-proven anti-photoaging natural products include caffeic acid, ferulic acid, resveratrol, celery Genistein, silymarin, tea polyphenols (such as epigallocatechin gallate), tannic acid, etc. Although natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/24A61K8/31A61K8/34A61K8/365A61K8/37A61K8/55A61K8/73A61K8/86A61K8/92A61Q17/04A61Q19/00
CPCA61K8/365A61K8/24A61K8/73A61K8/345A61K8/37A61K8/86A61K8/735A61K8/342A61K8/553A61K8/31A61K8/922A61Q17/04A61Q19/00
Inventor 周应军李丹李吉曾光尧邓旭曹伟
Owner CENT SOUTH UNIV
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