Lysobacter capsici CK09 with antagonistic effect and application of lysobacter capsici CK09
A capsicum lysobacteria, antagonistic technology, applied in the field of microorganisms, can solve the problems of chemical pesticide environmental pollution, threat to human health, enhanced chemical pesticide resistance, etc. Effect
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Embodiment 1
[0033] Example 1 Antagonistic effect of Lysobacter capsici CK09 on plant pathogenic fungi
[0034] Lysobacterium capsici CK09 with good antagonistic effect screened in the previous work was used as the strain to be tested to verify its antibacterial effect on pathogenic fungi. The pathogenic fungi selected here are preserved in our laboratory and are all plant pathogenic fungi. The specific steps are as follows:
[0035] (1) Select a single colony of Lysobacter capsici CK09 and inoculate it in R2A liquid medium, culture it on a shaker at 180rmp in an environment of 28°C until the late stage of logarithmic growth, and collect the bacterial liquid for later use when it stabilizes in the early stage;
[0036] (2) Pour the PDA solid medium onto the plate, and after the medium solidifies, transfer the activated pathogenic fungi to be tested to fresh PDA medium;
[0037] (3) Use tweezers to place the sterilized Oxford cup (inner diameter 0.78cm, outer diameter 0.8cm, height 1cm) ve...
Embodiment 2
[0044] Example 2 Antagonistic effect of Lysobacter capsici CK09 on plant pathogenic bacteria
[0045] The strain Lysobacter capsici CK09 was used as an antagonistic bacteria to verify its antibacterial effect on phytopathogenic bacteria. The bacteria selected here are the same phytopathogenic bacteria preserved in this laboratory, and all of them are Gram-positive Bacillus spp. Bacteria, the steps are as follows:
[0046] 1) Bacterial liquid preparation: inoculate the phytopathogenic bacteria to be tested and Lysobacter capsici CK09 into R2A liquid medium respectively, and cultivate them on a shaker at 180 rpm in an environment of 28°C until the late logarithmic growth period, and stabilize the early stage. Collect the bacterial solution for use;
[0047] 2) Prepare an R2A semi-solid medium with an agar concentration of 10 g / L, sterilize it and put it in an oven at 55 °C for use;
[0048] 3) Treatment of the indicator bacteria: Add 20 μL of the bacterial solution of the plant ...
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