Solen-derived oligopeptide as well as preparation method and application thereof
An oligopeptide and razor clam technology, applied in the field of stem cells, can solve the problem of low osteogenic transformation efficiency of bone marrow mesenchymal stem cells, and achieve the effects of overcoming the lack of cell transplantation sources, promoting osteogenic transformation, and accelerating osteogenic transformation.
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Embodiment 1
[0028] A preparation method derived from razor clam oligopeptide is as follows:
[0029] (1) Remove the shells of razor clams, wash them, mash them, grind them with a grinder, add distilled water, and then heat them in a water bath for heat treatment. The temperature of the water bath heating is 40°C for 15 minutes;
[0030] (2) Add protease to the product of step (1) for enzymolysis. During the enzymolysis process, ultrasonic-assisted enzymolysis is used. The protease added is trypsin. The amount of trypsin added is 5%, the pH is 7.2, and the enzymolysis temperature is 35°C, in ultrasonic-assisted enzymatic hydrolysis, the ultrasonic power is 50W;
[0031] (3) The enzymatic hydrolysis product obtained in step (2) was separated and purified by ultrafiltration, and dried at low temperature to obtain the required razor clam oligopeptide. The filtration temperature was 35° C. and the pressure was 11 psi.
Embodiment 2
[0033] A preparation method derived from razor clam oligopeptide is as follows:
[0034] (1) Remove the shells of razor clams, wash them, mash them, grind them with a grinder, add distilled water, and then heat them in a water bath for heat treatment. The temperature of the water bath heating is 48°C, and the time is 20 minutes;
[0035] (2) Add protease to the product of step (1) for enzymolysis. During the enzymolysis process, ultrasonic-assisted enzymolysis is used. The protease added is trypsin. The amount of trypsin added is 10%, the pH is 7.5, and the enzymolysis temperature is 35°C, in ultrasonic-assisted enzymatic hydrolysis, the ultrasonic power is 100W;
[0036] (3) The enzymatic hydrolysis product obtained in step (2) was separated and purified by ultrafiltration, and dried at low temperature to obtain the required razor clam oligopeptide. The filtration temperature was 35° C. and the pressure was 13 psi.
Embodiment 3
[0038] A preparation method derived from razor clam oligopeptide is as follows:
[0039] (1) Remove the shells of razor clams, wash them, mash them, grind them with a grinder, add distilled water, and then heat them in a water bath for heat treatment. The temperature of the water bath heating is 55°C and the time is 30 minutes;
[0040] (2) Add protease to the product of step (1) for enzymolysis. During the enzymolysis process, ultrasonic-assisted enzymolysis is used. The protease added is trypsin. The amount of trypsin added is 12%, the pH is 8.3, and the enzymolysis temperature is at 40°C, in ultrasonic-assisted enzymatic hydrolysis, the ultrasonic power is 120W;
[0041] (3) The enzymatic hydrolysis product obtained in step (2) was separated and purified by ultrafiltration, and dried at low temperature to obtain the required razor clam oligopeptide. The filtration temperature was 45°C and the pressure was 14psi.
[0042] Effects of razor clam oligopeptides on ALPase activi...
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