Improved segmented determination method for plant root activity
A technology of plant root system and measurement method, which is applied in the field of plant physiology, can solve the problem that there is no simple method for segmental measurement of plant root system vitality, and achieve the effect of short experiment time, fast speed and short time consumption
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Embodiment 1
[0028] Establishment of absorption peak:
[0029] After cleaning the rice root sample, blot the water on the surface of the root sample with absorbent paper, and use a double-sided blade to cut into sections from the root tip. Each section is 1 cm in length, and the root section is placed in 1ml of 0.6% (w / v) Put the TTC mixed solution in a 2ml centrifuge tube,. Put the centrifuge tube in a 40°C water bath for 30 minutes, when the root segment turns red, add 1mol L -1 1ml of sulfuric acid solution to stop the reaction. Suck out the mixed liquid of TTC and sulfuric acid in the centrifuge tube, rinse with RO water, blot the residual liquid on the surface of the root segment with absorbent paper, and add 1ml of 95% ethanol to the centrifuge tube. Cut the root section with scissors, and after leaching in a dark environment for 4 hours, the rice root section turns white to obtain an ethanol extract. Absorb the red ethanol extract from the upper layer, take the blank experiment a...
Embodiment 2
[0032] The subsection determination of the vitality of the 8cm root in the rice root system at different growth stages:
[0033] Will grow 15 days, 30 days, the root that length is 8cm in the rice root system of 45 days is taken off with double-sided blade. After cleaning the rice root sample, use absorbent paper to dry the surface moisture of the root sample, and use a double-sided blade to cut into segments from the root tip. When cutting into segments, the length of each segment is 1 cm. Weigh every 1 cm of the root segment on a balance. Root segments with a weight range of 20-30 mg were placed in a 2 ml centrifuge tube with 1 ml of TTC mixed solution with a final concentration of 0.6% (w / v). Place the centrifuge tube in a 40°C water bath and incubate for 30 minutes, when the root segment turns red, add 1mol L -11ml of sulfuric acid solution to stop the reaction. Suck out the mixed liquid of TTC and sulfuric acid in the centrifuge tube, rinse with RO water, blot the resid...
Embodiment 3
[0038] The root vigor of 4cm and 8cm in the root system of rice growing for 45 days was measured in sections:
[0039] Roots with a length of 4 cm and 8 cm in the rice root system grown for 45 days were removed with a double-sided blade. After cleaning the rice root sample, use absorbent paper to absorb the water on the surface of the root sample, and use a double-sided blade to cut into segments from the root tip. When cutting into segments, the length of each segment is 1 cm, and the root segments after cutting are obtained, and placed on the balance. Weigh every 1 cm root segment, put the root segment in the weight range of 20-30 mg in a 2 ml centrifuge tube with 1 ml final concentration of 0.6% (w / v) TTC mixed solution. Place the centrifuge tube in a 40°C water bath and incubate for 30 minutes, when the root segment turns red, add 1mol L -1 1ml of sulfuric acid solution to stop the reaction. Suck out the mixed liquid of TTC and sulfuric acid in the centrifuge tube, rinse...
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