Conductive multifunctional nanoprobe and preparation method and application thereof
A nano-probe and multi-functional technology, applied in the direction of material electrochemical variables, material analysis by electromagnetic means, instruments, etc., can solve the problems of poor conductivity, low catalytic activity, low loading of signal substances, etc.
Active Publication Date: 2020-09-22
CAPITAL NORMAL UNIVERSITY
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Problems solved by technology
However, the current common electrochemical immunoprobes have their own shortcomings, such as: poor conductivity, low catalytic activity, low loading of signal substances, etc.
Method used
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Embodiment 1
[0043] 1) Preparation of ZIF-8 nanoparticle probes co-modified by xanthine oxidase (XOD), 12-phosphotungstic acid, thionine, gold particles and CYFRA21-1 antibody
[0044] Add 0.2 mg of xanthine oxidase to 1 mL of 40 mM zinc acetate aqueous solution, and stir for 15 minutes. Subsequently, 1 mL of 0.16 mM 2-methylimidazole aqueous solution was added and stirred for 12 h. Centrifuge to obtain nanoparticles XOD@ZIF-8. 1mL 2.5mg mL -1 12-Sodium phosphotungstate aqueous solution with 2mL 5mg mL -1 The XOD@ZIF-8 dispersion was mixed, sonicated for 1.5h, stirred for 12h, centrifuged and washed to obtain 12-phosphotungstic acid-modified XOD@ZIF-8. The resulting product was added to 1 mg mL -1 in the thionine aqueous solution, stirred for 12 hours, and centrifuged to obtain nanoprobes encapsulated with thionine. Disperse the obtained product in 2 mL of secondary water, add 10 μL of 4% NaAuCl under stirring 4 aqueous solution, stirred for 3 h, then added 40 μL of 0.025% NaBH 4 Ice ...
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Abstract
The invention discloses a conductive multifunctional nanoprobe and a preparation method thereof. The nanoprobe comprises a biological enzyme, an MOFs material coating the biological enzyme, a heteropolyacid cluster (POM) and a signal molecule which fill in a pore channel of the MOFs material, gold particles growing on the surface of the MOFs material in situ, and a biological antibody connected with the gold particles. According to the conductive multifunctional nanoprobe, the MOFs material filled with POM has good hydrophilicity and is beneficial to ion transmission in an aqueous solution system so that the conductive multifunctional nanoprobe has good conductivity. Meanwhile, the improvement of hydrophilicity is also beneficial to the transmission of a catalytic substrate in a pore channel so that the catalytic activity is improved; POM has hydrogen peroxide catalytic activity and can be subjected to cascade catalytic reaction together with biological enzyme to amplify an electrochemical signal of a signal substance; after the pore channels of the MOFs material are filled with POM, the residual space can limit signal substance molecules so that the signal loading capacity of theprobe material is improved.
Description
technical field [0001] The invention belongs to the field of detection materials, and in particular relates to a metal-organic framework material-based conductive multifunctional nanometer probe and a preparation method thereof, and the use of the probe for electrochemically detecting tumor markers. Background technique [0002] Tumor markers are a class of proteins produced by tumor cells. Different types of tumor cells produce different tumor markers, and the concentrations of tumor markers in the blood of cancer patients at different stages are also different. Therefore, the detection of tumor markers is of great significance for the diagnosis, treatment and prognosis of cancer. At present, common analytical methods include enzyme-linked immunoassay, fluorescence detection, electrochemical immunoassay, etc. Electrochemical detection has attracted attention due to its advantages of rapid detection, small sample volume, and high sensitivity. However, the current common el...
Claims
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IPC IPC(8): G01N33/574G01N33/58G01N27/327G01N27/48
CPCG01N33/57484G01N33/581G01N33/587G01N27/3278G01N27/48
Inventor 马占芳许阳
Owner CAPITAL NORMAL UNIVERSITY