Template usable for preparing low-stimulation and high-compliance tissue engineering blood vessel and tissue engineering blood vessel

A technology of tissue engineering and compliance, applied in blood vessels, devices of human tubular structures, medical science, etc., can solve the problem of reducing the integration rate of blood vessels and tissues around the implantation site, the regeneration of tissue engineering tubes needs to be improved, and the stimulation of smooth muscle cells Overproliferation and other problems, to achieve the effect of reducing fiber exposure, good mechanical properties, and inhibiting intimal hyperplasia

Active Publication Date: 2020-09-25
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Second, the compliance of the blood vessel prepared by the above method is not good, and it will stimulate the excessive proliferation of smooth muscle cells after implantation in vivo, resulting in intimal hyperplasia;
[0007] Third, the outer surface of the tissue-engineered blood vessel prepared by subcutaneous i...

Method used

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  • Template usable for preparing low-stimulation and high-compliance tissue engineering blood vessel and tissue engineering blood vessel
  • Template usable for preparing low-stimulation and high-compliance tissue engineering blood vessel and tissue engineering blood vessel
  • Template usable for preparing low-stimulation and high-compliance tissue engineering blood vessel and tissue engineering blood vessel

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The low-stimulation, high-compliance tissue engineering blood vessel fabrication template provided by this embodiment includes an inner core from the inside to the outside, containing Co 2+ The anti-exposure layer and the skeleton body; the skeleton body includes 4 layers of reticular fiber skeleton layers and 3 layers of biodegradable fiber layers, and the inner and outer surfaces of the skeleton body are both mesh fiber skeleton layers.

[0065] The template preparation process includes the following steps:

[0066] 1. Select a silicone tube as the inner core, with an outer diameter of 2 mm and an inner diameter of 1 mm; the outer diameter of the inner core determines the inner diameter of the prepared tissue engineering blood vessel.

[0067] 2. With PLGA / CoCl 2 ·6H 2 O is the raw material, and the anti-exposure layer is prepared on the surface of the above-mentioned silicone tube by the spin coating method. The specific method is: insert a stainless steel rod with...

Embodiment 2

[0080] The template for making blood vessels for tissue engineering with low stimulation and high compliance provided by this embodiment includes an inner core from the inside to the outside, containing Co 2+ The anti-exposure layer and the skeleton body; the skeleton body includes 3 layers of reticular fiber skeleton layers and 2 layers of biodegradable fiber layers, and the inner and outer surfaces of the skeleton body are both mesh fiber skeleton layers.

[0081] The template preparation process includes the following steps:

[0082] 1. A polyurethane tube with an outer diameter of 4 mm and an inner diameter of 2 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared tissue engineering blood vessel.

[0083] 2. With PLGA / PEO / CoCl 2 ·6H 2 O is the raw material, adopts high-voltage electrospinning technology, and prepares the anti-exposure layer on the surface of the polyurethane tube. The specific method is: ins...

Embodiment 3

[0092] The low-stimulation, high-compliance tissue engineering blood vessel fabrication template provided by this embodiment includes an inner core from the inside to the outside, containing Co 2+ The anti-exposure layer and the skeleton body; the skeleton body includes 2 layers of reticular fiber skeleton layers and 1 layer of biodegradable fiber layer, and the inner and outer surfaces of the skeleton body are both mesh fiber skeleton layers.

[0093] The template preparation process includes the following steps:

[0094] 1. A silicone tube with an outer diameter of 5 mm and an inner diameter of 3 mm is used as the inner core, and the outer diameter of the inner core determines the inner diameter of the prepared tissue engineering blood vessel.

[0095] 2. Using P(3HB-co-4HB) / PEO / CoCl 2 ·6H 2 O is the raw material, adopts high-voltage electrospinning technology, prepares the anti-exposing layer on the surface of the silica gel tube, and the specific method is: insert a stai...

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Abstract

The invention belongs to the field of tissue engineering, and particularly relates to a template for preparing a low-stimulation and high-compliance tissue engineering blood vessel. The template comprises a skeleton main body (1), an inner core (2) and an anti-exposure layer (3), wherein the anti-exposure layer is arranged on the outer surface of the inner core; the skeleton main body is arrangedon the outer surface of the anti-exposure layer; the anti-exposure layer (3) comprises a degradable material and divalent cobalt ions; and the skeleton main body comprises a reticular fiber skeleton layer. The template has the beneficial effects that the anti-exposure layer is degraded to form a gap, and finally the surface of an inner cavity of a reticular fiber skeleton is coated with an acellular matrix, so that fiber exposure is reduced, and stimulation to blood coagulation reaction is reduced; the reticular fiber skeleton can enable the prepared tissue engineering blood vessel to have good mechanical properties; the bivalent cobalt ions in the anti-exposure layer can improve the compliance of the prepared tissue engineering blood vessel, reduce the occurrence rate of intimal hyperplasia and improve the tissue reproducibility after in-vivo implantation.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a template and a tissue engineering blood vessel which can be used for preparing low stimulation and high compliance tissue engineering blood vessels. Background technique [0002] Vascular disease is the disease with the highest fatality rate in the world. The occurrence of this disease is often due to the narrowing or blockage of blood vessels, which leads to reduced blood flow and lack of nutrients, which damages tissues or organs. It usually manifests as coronary heart disease, cerebrovascular disease, peripheral artery disease, etc. disease. According to the prediction of the World Health Organization, by 2030, the number of people dying of cardiovascular-related diseases in the world will increase to 23.3 million each year. Vascular transplantation is still a routine method for the treatment of such diseases. At present, the collection and use of patients' aut...

Claims

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Application Information

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IPC IPC(8): A61F2/06
CPCA61F2/062A61F2210/0004
Inventor 王恺孔德领智登科程曲汉黄子颀
Owner NANKAI UNIV
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