Pseudohemobacterium rhizosphere, its culture and isolation method and its use in tobacco flavoring

A bacillus and rhizosphere technology, applied in the field of rhizosphere Pseudohemobacterium and tobacco flavoring, can solve the problems of insufficient aroma components in cigarettes, and achieve the effects of improving cigarette smoking quality, reducing irritation, and increasing the richness of cigarette aroma

Active Publication Date: 2022-04-26
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the reduction of tar content in cigarettes, the problem of insufficient aroma components in cigarettes has become more prominent

Method used

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  • Pseudohemobacterium rhizosphere, its culture and isolation method and its use in tobacco flavoring
  • Pseudohemobacterium rhizosphere, its culture and isolation method and its use in tobacco flavoring
  • Pseudohemobacterium rhizosphere, its culture and isolation method and its use in tobacco flavoring

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Isolation, cultivation and identification of Pseudorivibacter rhizosphaerae C1-9

[0044] 1.1 Isolation of Pseudohemobacterium rhizosphere C1-9

[0045] Soil samples were taken from tobacco rhizosphere soil samples in Huize, Yunnan, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of the above soil sample, add 90mL of sterile water, shake on a shaker at 30°C and 180rpm for 30min; dilute the concentration to 10 with sterile water -1 、10 -2 、10 -3 、10 -4 、10 -5 Take 0.2mL of 5 concentrations of bacterial solution and spread it on the R2A plate culture medium, set up 3 parallel experiments for each concentration of bacterial solution, culture at 30°C for 48h, pick from the plate of each soil sample For different single colonies, after purification on the R2A plate medium, 20% of the volume of the bacterial solution was added to glycerol, and stored in a -80°C refrigerator for later use.

[0046] The isolated microorganisms were inocula...

Embodiment 2

[0066] Preparation of Tobacco Flavors from Pseudorivibacter rhizosphaerae C1-9

[0067] 1. Cultivation of Pseudohemobacterium rhizosphere C1-9

[0068] (1) Test tube slant culture

[0069] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate the rhizobacter Pseudomonas strain C1-9, and cultivate it at 28°C for 1 week to obtain the test tube strain;

[0070] (2) Seed cultivation

[0071] The seed culture medium is R2A liquid culture medium, and the formula is: 0.5g glucose, 0.5g yeast extract, 0.5g peptone, 0.5g acid hydrolyzed casein, 0.5g soluble starch, 0.3g sodium pyruvate, hydrogen phosphate Dip...

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Abstract

The invention discloses Pseudorivibacter rhizosphaerae C1-9, the 16S rDNA partial sequence thereof is shown in the sequence list, and its preservation number is CGMCC NO: 1.13864. The invention also discloses the method for cultivating and isolating the Pseudorivibacter rhizosphaerae C1-9 and the method for preparing the spice for tobacco. The Pseudohemobacterium rhizosphere of the present invention is a new bacterial strain, and the tobacco spice prepared by using it is used in cigarette flavoring, and the richness of the smoke aroma of the smoke is significantly increased, the delicateness of the smoke is increased, and the irritation is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to pseudohelicobacter rhizosphere, its separation method and application in tobacco flavoring. Background technique [0002] Flavors and fragrances play an important role in food, feed, cosmetics, chemical and pharmaceutical industries, and their sources mainly include natural and chemical synthesis. Among them, chemically synthesized flavors and fragrances account for about 85% of the entire product, but the chemically synthesized method has many by-products, low yield, high cost, and environmental pollution. With the advocacy of green industry, more and more people pay attention to natural flavors and fragrances. Natural flavors and fragrances mainly come from animals and plants, but the resources of animal and plant flavors are limited and the extraction cost is high, which is far from meeting the growing market demand. [0003] The tobacco industry is an important cons...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12P1/04A24B3/12C12R1/01
CPCC12N1/20C12N1/02C12P1/04A24B3/12C12R2001/01C12N1/205
Inventor 高莉者为段焰青刘秀明焦俊周兴奎曾熠程党立志
Owner CHINA TOBACCO YUNNAN IND
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