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Bovine-derived bacillus cereus and application thereof

A Bacillus cereus, bovine-derived technology, applied in bacteria, climate change adaptation, microorganisms, etc., to shorten the time of diagnosis and reduce the loss of disease

Active Publication Date: 2020-10-02
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But before this, there was no relevant report about the infection of the bacteria to the death of cattle.

Method used

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  • Bovine-derived bacillus cereus and application thereof
  • Bovine-derived bacillus cereus and application thereof
  • Bovine-derived bacillus cereus and application thereof

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Embodiment Construction

[0018] In 2018, cases of sudden cattle death occurred in many places in Tongliao City, Inner Mongolia. After we investigated the infectious diseases such as anthrax bacillosis and foot-and-mouth disease virus disease in two of the dead cattle, we conducted bacterial culture on their blood and found that there were colonies growing. The growing bacteria were isolated and identified, and finally identified as Bacillus cereus. Before this, there was no relevant report on the death of cattle infected by this bacterium, so its biological identification was carried out.

[0019] The strain was initially cultured in ordinary agar medium at 37°C for 48 hours, and there were wax drop-like colonies with a red center and an off-white periphery and irregular periphery. Microscopic examination shows that both ends of the bacterium are flat, the size is about 1.0×6.0um, and most of them are arranged in long chains, which is a Gram-positive bacterium ( figure 1 ). When observed under a mic...

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Abstract

The invention discloses bovine-derived bacillus cereus M001. The bovine-derived bacillus cereus M001 is collected in the China General Microbiological Culture Collection Center. The strain is separated from blood of cattle suffering from the sudden death disease; and the bacterial colony is a wax drop-like bacterial colony with red center, grey white periphery and irregular periphery. The strain can be seen by microscopic examination, two ends of the strain are flush, the size of the strain is about 1.0x6.0 [mu]m, and the strain is mostly arranged in a long chain mode and is Gram-positive bacteria. After special staining, the strain is placed under a microscope for observation, and parasporal crystals are not observed; a 16SrDNA sequence is as shown in a sequence table; the strain is sensitive to clindamycin and gentamicin drugs, is moderately sensitive to erythromycin, minocycline and other drugs, and is resistant to ceftazidime, aztreonam and other drugs; and the strain has pathogenicity and lethality for the cattle. The strain can be used as a bovine epidemic disease quarantine control sample or a bovine epidemic disease prevention vaccine.

Description

technical field [0001] The invention relates to a livestock microorganism and its use, namely a lethal bovine-derived bacillus cereus and its use. Background technique [0002] Bacillus cereus (Bacillus cereus. Frankland) belongs to the Bacillus family and belongs to the genus Bacillus cereus. This bacterium has similar biological characteristics to Bacillus thuringiensis and Bacillus anthracis, and it widely exists in the natural environment and food. It can produce antibacterial substances, inhibit the reproduction of harmful microorganisms, degrade the nutrients in the soil, and improve the ecological environment. Some Bacillus cereus can be used as plant growth promoters and microbial additives in animal feed. The bacterium is an opportunistic pathogenic bacterium, and it often causes vomiting or diarrhea in humans or animals in clinical practice. It is one of the common foodborne pathogenic bacteria. But before this, there was no relevant report about the infection an...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/085
CPCC12Q1/04C12R2001/085C12N1/205Y02A50/30
Inventor 马德慧王学理宋丽丽张玲艳贾伟娟
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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