Composition for improving colonization and activity of probiotics in nasal cavity and application of composition in nasal cavity nursing
A composition and technology of probiotics, applied in the field of colonization and active compositions, can solve the problems of not mentioning the survival rate or colonization rate of probiotics, disturbing the normal concentration of surface active substances, and failing to keep the nasal mucosa moist, etc., to achieve Keeping the nasal cavity moist, relieving nasal dryness, beneficial to the effect of relief and treatment
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experiment example 1
[0031] Experimental example 1 Study on the colonization and survival promotion effect of probiotics by compositions of different components
[0032] Different compositions were formulated to enhance the colonization and activity of probiotics in the nasal cavity.
[0033] Each active ingredient and dosage are shown in Table 1 below, and the preparation method is as follows:
[0034] Dissolve hyaluronic acid in 100ml of double-distilled water, add β-glucan oligosaccharides and vitamin C, stir to dissolve, filter and sterilize with a 0.22um filter membrane, freeze-dry to obtain probiotics that can improve the colonization and activity of probiotics in the nasal cavity combination.
[0035]
[0036] The effects of the above compositions on the survival and colonization of probiotics were verified.
[0037] , probiotic survival rate
[0038]The low-temperature-preserved Lactobacillus acidophilus was taken out and subcultured twice for strain activation. Receive the activate...
experiment example 2
[0045] Experimental Example 2 To study the effect of different nasal cavity care solutions on the nasal cavity
[0046] Mix the composition for improving the colonization and activity of probiotics in the nasal cavity mentioned in Experimental Example 1 above with probiotics and other ingredients to make a nasal cavity care solution, the method is as follows:
[0047] 1. Dissolve hyaluronic acid in 100ml double-distilled water, add β-glucan oligosaccharides, vitamin C and NaCl, stir to dissolve, filter and sterilize with a 0.22um filter membrane, and adjust the pH of the solution to 6.0-7.0;
[0048] 2. Cultivate Lactobacillus acidophilus to the stationary phase, calculate the number of live bacteria, and centrifuge to obtain probiotic cells;
[0049] 3. Dissolve the probiotic cells with the solution in the above step 1, so that the content of the probiotics is 10 8 CFU / mL, the final pH of the solution is guaranteed to be 6.0-7.0, and different nasal cavity care solutions are...
experiment example 3
[0066] Experimental Example 3 Probiotic Type Screening
[0067] 1. Sample preparation:
[0068] 1.1. Dissolve 0.5g of hyaluronic acid with a molecular weight of 70wDa in 100ml of double distilled water, add 1.5g of β-glucan oligosaccharides, 2g of vitamin C and 0.9g of NaCl, stir to dissolve, filter and sterilize the solution with a 0.22um filter membrane The pH is 6.0-7.0;
[0069] 1.2. Cultivate Streptococcus and Corynebacterium pseudodiphtheriae until the stationary phase, calculate the number of viable bacteria, and centrifuge to obtain probiotic cells;
[0070] 1.3. Dissolve the Streptococcus thallus and Corynebacterium pseudodiphtheriae thallus with the solution of the above step 1.1, so that the content of probiotics is 10 8 CFU / mL, the final pH of the solution is guaranteed to be 6.0-7.0, and different nasal cavity care solutions are obtained.
[0071] 2. Experimental method:
[0072] The mice were modeled and treated according to the method in Experimental Example...
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