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Lactobacillus brevis strain and its culture method and use

A kind of Lactobacillus brevis, strain technology, applied in the field of novel Lactobacillus brevis strain and its cultivation, can solve the problems such as the output can not reach high yield and the like

Active Publication Date: 2021-05-14
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the strain uses brown rice flour as a medium, although the cost is reduced, but its yield still cannot meet the requirement of high yield.

Method used

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  • Lactobacillus brevis strain and its culture method and use
  • Lactobacillus brevis strain and its culture method and use
  • Lactobacillus brevis strain and its culture method and use

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preparation example Construction

[0057] The present invention further provides a preparation method of Chinese cabbage extract, which includes mixing Chinese cabbage waste with water, heating at high temperature, extracting for a certain period of time, and pressing to obtain Chinese cabbage extract.

[0058] The Chinese cabbage extract in the present invention refers to a liquid extracted from Chinese cabbage waste raw materials. In the case that the raw material is fresh Chinese cabbage raw material, the Chinese cabbage extract is obtained by pressing the raw material or extracting a mixture of the raw material and water. When the raw material is a dried cabbage raw material, the Chinese cabbage extract generally needs to be extracted by adding water to the dried cabbage raw material and heating it.

[0059] Preferably, the cabbage waste is mixed with water at a weight ratio of 0.8-1.2:0.8-1.2, more preferably at a weight ratio of 0.8-1.2:1, more preferably at a weight ratio of 1:1. The cabbage waste needs...

Embodiment

[0067] The present invention isolates and screens out a lactic acid bacterial strain with high yield of GABA from pickles, and the bacterial strain is identified as Lactobacillus brevis, and its electron microscope picture is as follows: figure 1 As shown, named Lactobacillus brevis PL6-1. The strain is Gram-positive, catalase-negative, short-rod-shaped, and the fermentation pH is 4.81. The source is radish kimchi. Gram-stained microscopic results are as follows: figure 2 shown. The strain has been deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on May 22, 2020, and the preservation number is: CGMCC No.19868.

[0068] 1. Determination of Physiological and Biochemical Properties

[0069] The API 50 CHL reaction results of bacterial strain PL6-1 are shown in Table 1:

[0070] Table 1 API 50 CHL reaction results of bacterial strain PL6-1

[0071]

[0072]

[0073] Note: "+" stands for positive, "-" stands for...

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Abstract

The invention discloses a strain of Lactobacillus brevis and its cultivation method and application. The Lactobacillus brevis strain is Gram-positive, catalase-negative, and short-rod-shaped Lactobacillus brevis isolated from radish kimchi. 1, which contains thermostable glutamic acid decarboxylase. At the same time, the present invention develops a Chinese cabbage extract medium for improving its fermentation performance and a method for producing γ-aminobutyric acid based on the strain. The Chinese cabbage extract medium comprises 20-35 parts by weight of Chinese cabbage extract, 2-6 parts by weight of glucose, 0.2-4.5 parts by weight of yeast extract and 0.3-2.2 parts by weight of metal salts. The culture medium of the invention can greatly reduce the production cost of GABA, and at the same time remarkably improve the output and conversion rate of GABA.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a novel strain of Lactobacillus brevis and its cultivation method and application. Background technique [0002] γ-aminobutyric acid (γ-aminobutyric acid, GABA) is a naturally occurring four-carbon non-protein amino acid, which has regulatory effects on central nervous diseases, hypertension, cancer, diabetes, and human immunity. In addition to plant enrichment, food-grade GABA is mainly produced by microbial glutamate decarboxylase (glutamate decarboxylase, GAD) as a substrate glutamate. Lactic acid bacteria are generally considered to be safe and non-toxic, and have good probiotic effects on the human body. The production of GABA by fermentation of lactic acid bacteria is a relatively safe and ideal production method, with the advantages of low cost, high content and safe use in food. [0003] The use of microbial fermentation to produce γ-aminobutyric acid is an efficien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P13/00A23L19/20A23L2/38C12R1/24
CPCC12N1/20C12P13/005A23L19/20A23L29/065A23L2/54
Inventor 刘文丽李华敏孙舒扬任向亮
Owner LUDONG UNIVERSITY