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Gene marker panels and their applications for the assessment of endometrial receptivity

An endometrial and receptive technology, applied in the field of biomedicine, can solve the problems of finding and infertile patients, unable to accurately and effectively assess the receptivity of the endometrium, etc.

Active Publication Date: 2021-10-22
UNIMED BIOTECH SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is that the existing technology cannot accurately and effectively evaluate the endometrial receptivity, and it is difficult to find the best WOI for infertile patients

Method used

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  • Gene marker panels and their applications for the assessment of endometrial receptivity
  • Gene marker panels and their applications for the assessment of endometrial receptivity
  • Gene marker panels and their applications for the assessment of endometrial receptivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Sample collection, transportation, quality control

[0049]1. The subject or volunteer shall be sampled by a gynecologist or a professionally qualified technician. The sample shall include not less than 1mg of endometrial tissue and not less than 4uL of uterine cavity fluid. Immediately after the biopsy was completed, the sample was added to a preservation tube containing RNA preservation solution (Qiagen RNA Later), and stored at -20°C or -80°C.

[0050] 2. Sample transportation: Use ice packs or dry ice to transport samples to designated laboratories.

[0051] 3. Sample quality control: The quality control content includes sample information, sample integrity, and the actual temperature measured when the sample is received.

[0052] 4. Specific sample collection and grouping are shown in Table 2.

[0053] Table 2 Number of samples in different periods of endometrial receptivity

[0054]

Embodiment 2

[0055] Example 2 RNA sequencing

[0056] 1. Extract total RNA from endometrial biopsy samples.

[0057] 2. Use Agilent2100 to analyze the integrity of RNA, RIN>7.0, 28S / 18S>1.2 is qualified. The RNA concentration and purity were accurately quantified using Qubit. The OD260 / OD280 was between 1.8 and 2.2, and the RNA extraction amount was greater than 2ug, which was considered qualified.

[0058] 3. Use the poly(A) purification method or the rRNA removal purification method to enrich and purify the mRNA.

[0059] 4. Use KAPA Stranded RNA-Seq Library Preparation Kit for RNA library construction.

[0060] 5. Use Agilent2100 to analyze the distribution of library fragments, and use Qubit to accurately quantify the library concentration.

[0061] 6. Use illumina Hiseq2000 on-machine sequencing.

Embodiment 3

[0062] Example 3 Screening of characteristic gene markers related to endometrial receptivity assessment

[0063] 1. Data quality control: After the original data is off the machine, the quality control of the sequencing results is carried out through the fastp software. The quality control standard is that the read length is not less than 75, and the rest of the parameters are default (the original data is called RawData, and the data after quality control is called CleanData. ).

[0064] 2. Ribosome data deletion: rRNA content can reflect the quality of library construction to a certain extent, so before comparing the reference genome, use sortmerna to compare CleanData to the ribosome library, remove the reads on the comparison, and get de - rRNA CleanData default filter parameter is 1e-5.

[0065] 3. Reference genome comparison: compare de-rRNA CleanData to the reference genome or transcriptome, calculate the comparison efficiency, and perform randomness analysis and inser...

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PUM

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Abstract

The invention belongs to the field of biomedicine and discloses a combination of gene markers for evaluating endometrium receptivity. The invention provides a nucleic acid composition, including DNA and RNA corresponding to 515 endometrial receptivity-related characteristic genes. The invention also discloses the application of the above gene marker combination in preparing endometrial receptivity testing products. The present invention includes a molecular biological method for evaluating endometrial receptivity based on the gene expression status of uterine biopsy sample cells. Guidance can significantly improve the success rate of in vitro fertilization or embryo implantation.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a combination of gene markers for evaluating endometrium receptivity and its application. Background technique [0002] According to data released by the China Population Association and the National Family Planning Commission, the infertility rate of couples of childbearing age in China has climbed from 2.5% to 3% 20 years ago to about 12% to 15% in recent years, and the number of patients has exceeded 50 million. Among the 50 million patients, 50% are women, 40% are men, and 10% are due to common causes between husband and wife. And with environmental pollution, delayed childbearing age, life pressure and other reasons, the number of infertile couples is still increasing. [0003] Studies have shown that altered endometrial receptivity may be one of the main factors leading to reproductive disorders. Endometrial receptivity refers to the ability of the endometrium to accept embryos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11G16B35/20G16B40/00G16B20/30G16B25/20
CPCC12Q1/6883C12Q2600/158G16B20/30G16B25/20G16B35/20G16B40/00
Inventor 吴涵陈究成李庆陈伟坚刘虎陈家生
Owner UNIMED BIOTECH SHANGHAI CO LTD
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