Detection kit based on Trinder reaction and application of detection kit

Abstract

The invention discloses a detection kit based on Trinder reaction and application of the detection kit. The invention belongs to the technical field of clinical chemical detection. The kit consists ofa reagent R1 and a reagent R2, wherein the R1 reagent and the R2 reagent contain enzymes for catalyzing a to-be-detected object to generate hydrogen peroxide, the reagent R1 further contains a buffersolution, a preservative and peroxidase, the reagent R2 contains a buffer solution, a preservative, peroxide and a chromogen substrate, the concentration of peroxidase in the reagent R1 is 100-150 KU / L, and the reagent R1 further contains 4-aminoantipyrine with the concentration of 0.5-1 g / L. the peroxidase and the 4-aminoantipyrine in the reagent R1 can be used for removing clinical drugs capable of participating in the Trinder reaction in a to-be-detected object before the Trinder reaction, so that a detection result is more accurate. According to the invention, other substances do not needto be additionally added into a reaction system, and other impurities cannot be introduced; the method is simple and efficient, can be widely applied to various clinical chemical detections, avoids the interference of clinical drugs, enhances the accuracy of detection results, and also improves the functional sensitivity of the kit.

Description

technical field [0001] The invention belongs to the technical field of clinical chemical detection, and in particular relates to a detection kit based on Trinder reaction and its application. Background technique [0002] Trinder reaction is also known as "coupling end-point colorimetric method", and its principle is the hydrogen peroxide (H 2 o 2 ) in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD), a red quinone imine compound can be generated, and the analyte can be calculated by measuring the difference in absorbance before and after the reaction concentration. Trinder reaction is used in many clinical biochemical testing items, such as glucose, triglyceride, cholesterol, uric acid, high-density lipoprotein, low-density lipoprotein, free fatty acid, etc. For example, Chinese patent CN106191213B discloses a free fatty acid assay kit and its preparation method. The reaction principle is that free fatty acid in human serum and coenzyme A react under the ac...

AI Technical Summary

Problems solved by technology

The influence of drugs on test results not only leads to misinterpretation and misdiagnosis of test results, but also increases unnecessary inspections, so it is particularly important to solve the problem of drug interference in testing

Clinically common drugs such as: calcium dobesilate (for the treatment of microvascular diseases, etc.), phensulfame (hemostatic drug), methyldopa (antihypertensive drug), levodopa (for the treatment of Parkinson's syndrome) sign), dopamine (neurotransmitter), etc., due to their strong reducing properties or similar structure to the chromogen substrate in the Trinder reaction, hydrogen peroxide will be consumed during the Trinder reaction, resulting in low measured values ​​of the analyte

And these drugs are stable in structure and not easy to be destroyed or degraded, so they will interfere with clinical chemical detection based on Trinder reaction and affect the measurement results

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