Detection kit based on Trinder reaction and application of detection kit

A detection kit and reagent technology, applied in the field of clinical chemical detection, can solve the problems of affecting the measurement results, misdiagnosis, and misinterpretation of test results, etc.

Inactive Publication Date: 2020-10-23
WUHAN LIFE ORIGIN BIOTECH LTD
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The influence of drugs on test results not only leads to misinterpretation and misdiagnosis of test results, but also increases unnecessary inspections, so it is particularly important to solve the problem of drug interference in testing
Clinically common drugs such as: calcium dobesilate (for the treatment of microvascular diseases, etc.), phensulfame (hemostatic drug), methyldopa (antihypertensive drug), levodopa (for the treatment of Parkinson's syndrome) s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection kit based on Trinder reaction and application of detection kit
  • Detection kit based on Trinder reaction and application of detection kit
  • Detection kit based on Trinder reaction and application of detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 creatinine detection kit

[0040] This embodiment provides a creatinine detection kit, which is compared with a conventional creatinine detection kit, wherein the detection kit of the present invention is the test group 1, and the conventional creatinine detection kit is the control group 1, and the formula details as follows:

[0041]

[0042] Wherein the test group one compared with the control group one, added 150KU / L of peroxidase and 1g / L of 4-aminoantipyrine in the R1 reagent component to be used for consuming medicine, simultaneously due to peroxidase The stability of catalase is poorer than that of catalase, so test group one also added an appropriate amount of metal ion chelating agent HEDTA and surfactant GENAPOLX-080 in the R1 reagent components to ensure the stability of peroxidase; test Group 1 also added 1g / L DHBS to the R2 reagent component instead of TOOS in the control group 1 R1 reagent component as the chromogen substrate.

[0043] T...

Embodiment 2

[0055] Embodiment 2 free fatty acid detection kit

[0056] This embodiment provides a free fatty acid detection kit, and compared with the conventional free fatty acid detection kit, wherein the detection kit of the present invention is the test group two, and the conventional creatinine detection kit is the control group two, The formula is as follows:

[0057]

[0058]

[0059] Compared with the control group two, the test group two added 100KU / L of peroxidase and 0.5g / L of 4-aminoantipyrine to the R1 reagent components to consume the drug, and there was no Contain metal ion chelating agent and surfactant; Added 1g / L HAOS in the R2 reagent component to replace the TOOS in the control group—R1 reagent component as the chromogen substrate. The determination method was the same as that in Example 1, wherein the volume of each sample was 5 μL, and 240 μL of R1 reagent and 60 μL of R2 reagent were added. Measure the performance of the control group 2 and the test group 2 ...

Embodiment 3

[0072] Example 3 Triglyceride Detection Kit

[0073] This embodiment provides a triglyceride detection kit, and compared with the conventional triglyceride detection kit, wherein the test group three is the detection kit of the present invention, and the control group three is the conventional triglyceride detection kit The specific formula of the detection kit is as follows:

[0074]

[0075] Wherein test group three is compared with control group three, in the R1 reagent component, has added the 4-aminoantipyrine of 4mmol / L (0.81g / L), promptly added peroxidase and simultaneously before Trinder reaction 4-aminoantipyrine, while the control group 3 is 4-aminoantipyrine added to the R2 reagent components for Trinder reaction, while the test group 3 added DHBS to the R2 reagent components to replace the control group The p-chlorophenol in the three R1 reagent components is used as the chromogen substrate. The determination method is as shown in Example 1, wherein the volume...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detection kit based on Trinder reaction and application of the detection kit. The invention belongs to the technical field of clinical chemical detection. The kit consists ofa reagent R1 and a reagent R2, wherein the R1 reagent and the R2 reagent contain enzymes for catalyzing a to-be-detected object to generate hydrogen peroxide, the reagent R1 further contains a buffersolution, a preservative and peroxidase, the reagent R2 contains a buffer solution, a preservative, peroxide and a chromogen substrate, the concentration of peroxidase in the reagent R1 is 100-150 KU/L, and the reagent R1 further contains 4-aminoantipyrine with the concentration of 0.5-1 g/L. the peroxidase and the 4-aminoantipyrine in the reagent R1 can be used for removing clinical drugs capable of participating in the Trinder reaction in a to-be-detected object before the Trinder reaction, so that a detection result is more accurate. According to the invention, other substances do not needto be additionally added into a reaction system, and other impurities cannot be introduced; the method is simple and efficient, can be widely applied to various clinical chemical detections, avoids the interference of clinical drugs, enhances the accuracy of detection results, and also improves the functional sensitivity of the kit.

Description

technical field [0001] The invention belongs to the technical field of clinical chemical detection, and in particular relates to a detection kit based on Trinder reaction and its application. Background technique [0002] Trinder reaction is also known as "coupling end-point colorimetric method", and its principle is the hydrogen peroxide (H 2 o 2 ) in the presence of 4-aminoantipyrine (4-AAP) and peroxidase (POD), a red quinone imine compound can be generated, and the analyte can be calculated by measuring the difference in absorbance before and after the reaction concentration. Trinder reaction is used in many clinical biochemical testing items, such as glucose, triglyceride, cholesterol, uric acid, high-density lipoprotein, low-density lipoprotein, free fatty acid, etc. For example, Chinese patent CN106191213B discloses a free fatty acid assay kit and its preparation method. The reaction principle is that free fatty acid in human serum and coenzyme A react under the ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/62C12Q1/60C12Q1/61C12Q1/54C12Q1/48C12Q1/34C12Q1/44C12Q1/28C12Q1/26G01N21/31G01N21/75
CPCC12Q1/26C12Q1/28C12Q1/34C12Q1/44C12Q1/48C12Q1/485C12Q1/54C12Q1/60C12Q1/61C12Q1/62C12Q2326/96G01N21/31G01N21/75G01N2333/90203G01N2333/90235G01N2333/904G01N2333/90683G01N2333/908G01N2333/91051G01N2333/91057G01N2333/91215G01N2333/916G01N2333/92G01N2333/978
Inventor 梁艳赵畅龚婷吴年芬凡速朋舒芹张雪娇赵愿安
Owner WUHAN LIFE ORIGIN BIOTECH LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap