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SNP molecular marker located on pig chromosome 9 and related to scrotal hernia and application

A technology of molecular markers and scrotal hernia, applied in the fields of molecular biotechnology and molecular markers, can solve problems such as large confidence intervals, complex genetic mechanisms, and inability to accurately anchor disease-causing genes

Active Publication Date: 2020-10-23
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Genetic defects in pigs are a type of structural or functional defects caused by genetic factors. Generally, they are controlled by multiple genes on the genome, and the genetic mechanism is complex.
Previous studies have used microsatellite markers and other methods to identify some QTLs affecting porcine scrotal hernia, but because of the large confidence interval (greater than 20Mb) and containing dozens of genes, it is impossible to accurately anchor disease gene

Method used

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  • SNP molecular marker located on pig chromosome 9 and related to scrotal hernia and application
  • SNP molecular marker located on pig chromosome 9 and related to scrotal hernia and application
  • SNP molecular marker located on pig chromosome 9 and related to scrotal hernia and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Experimental animals

[0041] The experimental pig group used in the present invention is 1099 purebred Landrace pigs from the Breeding Pig Branch of Wen's Food Group Co., Ltd., which is the core group of the Breeding Pig Branch.

[0042] In this experiment, the Landrace pigs in this resource group were selected. The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0043] (2) Sample collection

[0044] The above-mentioned piglet docked tail and ear tissues were collected and soaked in ethanol solution with a volume fraction of 75%, and stored in a -20°C refrigerator for later use.

[0045] (3) 50K SNP typing in the whole pig genome

[0046] From the ear tissue or docked tail tissue collected from each individual of 1047 Landrace pigs selected from the above resource groups, the whole genome DNA was extracted by the standard phenol-chloroform method, and each sampl...

Embodiment 2

[0058] Example 2 Target DNA sequence amplification and sequencing

[0059] (1) Primer design

[0060] The DNA sequence of SEQ ID NO: 1 on pig chromosome 9 was downloaded from the Ensembl website (http: / / asia.ensembl.org / index.html), and primers were designed using the primer design software primer premier 6.0. The DNA sequences of the designed primers are as follows:

[0061] P001-F: 5'-CTGGCTCCAGAGTGAGGAGA-3',

[0062] P002-R: 5'-GACACAACCCAAGAGGTCCA-3';

[0063] (2) PCR amplification

[0064] Add 1 μL of DNA template, 3.4 μL of double distilled water, 5 μL of 2×Tag PCR StanMix with Loading Dye, and 0.3 μL of primers P001-F and P002-R into a 10 μL reaction system. The PCR reaction conditions were as follows: 5 minutes of pre-denaturation at 94°C, 35 cycles of denaturation at 94°C for 30 s, annealing at 58°C for 30 s, extension at 72°C for 45 s, and finally 5 min at 72°C.

[0065] (3) DNA sequence determination

[0066] DNA sequence sequencing identification: carried out...

Embodiment 3

[0069] Example 3 SNP site g.208G>A effect analysis of molecular markers

[0070] According to Table 1, for the scrotum hernia of pigs, the frequency (AA) of the SNP site g.208G>A dominant allele is 0.167 in diseased individuals and 0.627 in normal pigs; the favorable allele The frequency of A was 0.33 in individuals with scrotal hernia and 0.79 in normal pig individuals. Therefore, through molecular marker-assisted selection and gradually eliminating pigs with genotype GG in the population, the allele frequency of allele A can be significantly increased, the incidence of scrotal hernia in breeding pigs can be reduced, and animal welfare can be improved while reducing breeding losses. , to speed up the improvement of genetic defects in pigs so as to effectively improve the economic benefits of pig breeding.

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Abstract

The invention belongs to the technical field of molecular biotechnology and molecular markers, and particularly relates to an SNP molecular marker located on a pig chromosome 9 and related to scrotalhernia and application. The SNP site of the SNP molecular marker located on the chromosome 9 of the pig and related to scrotal hernia is corresponding to the 128013229th G > A mutation on the chromosome 9 of the reference sequence 11.1 version of the international pig genome. By optimizing the dominant allele of the SNP, the frequency of the dominant allele can be increased generation by generation, the occurrence rate of the swine scrotal hernia is reduced, the breeding loss is reduced, meanwhile, the animal welfare is improved, the disease-resistant breeding progress of swine genetic defectsis accelerated, and therefore the economic benefits of swine breeding are effectively improved.

Description

technical field [0001] The invention belongs to the technical fields of molecular biotechnology and molecular markers, and in particular relates to a SNP molecular marker located on pig chromosome 9 and related to the occurrence of scrotal hernia and its application. Background technique [0002] Genetic defects in pigs are a type of structural or functional defects caused by genetic factors. Generally, they are controlled by multiple genes in the genome, and the genetic mechanism is complex. In pig production, the more common genetic defects include scrotal / inguinal hernia, umbilical hernia, lock anus, and splayed feet. Because of its low occurrence rate, breeders often pay little attention to it, and the research progress is slow. As a more prominent genetic defect, scrotal hernia is caused by defects in the musculature around the inguinal canal, and the contents of the abdomen will fall into the scrotum, resulting in slow growth of affected individuals, low feed remunera...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6883C12N15/11
CPCC12Q1/6888C12Q1/6883C12Q2600/124C12Q2600/156
Inventor 吴珍芳杨杰庄站伟吴杰杨明蔡更元郑恩琴曾海玉李紫聪顾婷徐铮
Owner SOUTH CHINA AGRI UNIV
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