Liposome, dispersion liquid containing liposome, and preparation methods and application of liposome and dispersion liquid

A liposome and dispersion technology, applied in the medical field, can solve problems such as the source of human serum albumin and the limitation of treatment costs

Active Publication Date: 2020-10-27
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although albumin dialysis can effectively remove water-soluble toxins and protein-bound toxoids, correct the internal environment disorder of patients, and improve clinical symptoms, its clinical application is limited by the source of human albumin and the cost of treatment.

Method used

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  • Liposome, dispersion liquid containing liposome, and preparation methods and application of liposome and dispersion liquid
  • Liposome, dispersion liquid containing liposome, and preparation methods and application of liposome and dispersion liquid
  • Liposome, dispersion liquid containing liposome, and preparation methods and application of liposome and dispersion liquid

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preparation example Construction

[0041] The preparation method provided by the present invention may include: A) dispersing each raw material component in a solvent to provide a premix. The solvent used in the preparation method can generally be a good solvent for the raw material components, usually an organic solvent, specifically a halogenated alkane solvent, an alcohol solvent, etc. In a preferred embodiment of the present invention, the solvent It can be a combination of one or more of dichloromethane, chloroform, methanol or ethanol, etc., and the amount of solvent used can be 25-75mL / 1g solid, 25-35mL / 1g solid, 35-35mL / 1g solid, etc. 45mL / 1g solid, 45-55mL / 1g solid, 55-65mL / 1g solid, or 65-75mL / 1g solid.

[0042] In the preparation method provided by the present invention, it may also include: B) removing the solvent in the premix provided in step A), so as to provide a phospholipid film. The method for removing the solvent should be known to those skilled in the art, for example, the solvent in the p...

Embodiment 1

[0052] Preparation of liposomes:

[0053] Soybean lecithin liposomes and linoleic acid-modified liposomes were prepared by film hydration method.

[0054] In the liposomes modified with linoleic acid, soybean lecithin (analytical pure, Shanghai Aiweite Pharmaceutical Technology Co., Ltd.), cholesterol (analytical pure, Shanghai Yuanju Biotechnology Co., Ltd.), Tween-80 (analytical pure, Shanghai Yuanju Biotechnology Co., Ltd.) The ratio between Yuanju Biotechnology Co., Ltd.) and linoleic acid (analytical grade, Sigma) is 9:3:4:3, in soybean lecithin liposomes (that is, liposomes without linoleic acid modification) , the ratio between soybean lecithin, cholesterol, and Tween-80 is 12:5:3, and each raw material component is dissolved in dichloromethane, and vacuum evaporated overnight. Then, the obtained dry film was hydrated with PBS buffer solution (addition amount: 25 mL / 1 g solid), and homogenized with a high-pressure homogenizer, with a homogenization pressure of 60 bar a...

Embodiment 2

[0056] Characterization of linoleic acid liposomes:

[0057] Stain with phosphotungstic acid, and observe the morphology of linoleic acid liposomes under a transmission electron microscope. The particle size and potential of liposomes were measured using a Zetasizer instrument.

[0058] The diameter range of most liposomes is 100-200nm, the distribution range is about 109.1±60.28nm, the average particle size of linoleic acid liposome is about 116.4±0.99nm, and the dispersion index (PDI) is 0.15±0.01( figure 2 ). The structure of phospholipid bilayer wrapped water-based inner core can be seen under transmission electron microscope ( image 3 ). The liposome solution was stable at room temperature for 14 days without precipitation, and the stability of the liposome was partly due to its negative surface potential (-6.96 ± 0.68mV) ( Figure 4 ).

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Abstract

The invention relates to the field of medical treatment, in particular to lipidosome, dispersion liquid containing the lipidosome, and preparation methods and application of the lipidosome and the dispersion liquid. The lipidosome provided by the invention comprises phospholipid, a liquidity buffer agent and linoleic acid. The lipidosome provided by the invention comprises linoleic acid; and through modification of the lipidosome by linoleic acid, the lipidosome has effectively improved clearing effect on protein binding toxoids related to uremia and liver failure; and when the lipidosome is applied to dialysate in the blood purification therapy, the lipidosome has the advantages of high efficiency, relatively low cost and the like.

Description

technical field [0001] The invention relates to the medical field, in particular to a liposome, a dispersion containing the liposome, their preparation method and application. Background technique [0002] Protein-bound toxoids refer to the toxins that exist mostly in the form of binding in plasma and accumulate abnormally in pathological conditions. Since the free level of protein-bound toxoids in the blood is low, the toxins bound to proteins cannot pass through the dialysis membrane. Difficult to remove by conventional hemodialysis techniques. Uremic protein-binding toxins (PBUTs) accumulate in patients with end-stage renal disease and are associated with an increased incidence of uremic complications, among which indoxyl sulfate and p-cresol sulfate have been confirmed to be associated with chronic kidney disease (CKD) patients associated with an increased incidence of all-cause mortality and cardiovascular disease. The removal of uremic PBUTs is a difficult problem in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/24A61K47/12A61P7/08
CPCA61K9/1277A61K47/24A61K47/12A61P7/08
Inventor 丁峰沈玥王宜峰史媛媛
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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