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A kind of production preparation technology of trehalose

A preparation process, trehalose technology, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of limited scope of application, inability to completely remove, etc., achieve consistent process, easy and excellent separation effect, and improve conversion rate effect

Active Publication Date: 2021-07-02
JIANGSU OGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, for the improvement of trehalose content, the methods of ion exchange desalination and chromatographic separation and purification are generally adopted, and the prepared trehalose content is about 98%; or refined by alcohol separation, but alcohol separation needs to use some chemical reagents, such as Alcohol substances, which have a certain amount of retention in trehalose products, cannot be completely removed. Although the content of trehalose in the product has been increased, the scope of application of trehalose products has been limited to a certain extent. Trehalose products cannot be used in cosmetics

Method used

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  • A kind of production preparation technology of trehalose
  • A kind of production preparation technology of trehalose
  • A kind of production preparation technology of trehalose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] S1: Starch liquefaction: Mix starch and water to prepare starch slurry, then adjust the pH value of the starch slurry to 5.4, add α-amylase, mix well, heat up to 105°C for 5 minutes, and then flash evaporate to 94 DEG C and laminar flow 60min, control DE value 2, inactivate enzyme, obtain liquefaction liquid; The starch is 84.3% cornstarch; The mass concentration of described starch slurry is 20%; The addition amount of α-amylase is starch 0.02% by mass.

[0061] S2: Trehalase conversion: Take the liquefied liquid, add starch debranching enzyme and trehalose converting enzyme, and enzymatically hydrolyze for 24 hours under the conditions of pH 5.5 and temperature 45° C. to obtain trehalose conversion liquid. The trehalose inverting enzyme includes trehalose hydrolase and trehalose synthetase; the mass of the trehalose inverting enzyme is 1% of the starch mass.

[0062] S3: Compound enzyme conversion: take trehalose conversion solution, add glucoamylase and compound enz...

Embodiment 2

[0069] S1: Starch liquefaction: Mix starch and water to prepare starch slurry, then adjust the pH value of the starch slurry to 5.6, add α-amylase, mix well, heat up to 108°C for 6 minutes, and then flash evaporate to 97°C and laminar flow for 75min, control the DE value to 3, and inactivate the enzyme to obtain a liquefied liquid; the starch is 85.9% potato starch; the mass concentration of the starch slurry is 25%; the amount of α-amylase added is the starch mass 0.03%.

[0070] S2: Trehalase conversion: Take the liquefied liquid, add starch debranching enzyme and trehalose converting enzyme, and enzymatically hydrolyze for 36 hours under the conditions of pH 6 and temperature 50° C. to obtain trehalose conversion liquid. The trehalose inverting enzyme includes trehalose hydrolase and trehalose synthetase; the mass of the trehalose inverting enzyme is 2% of the starch mass.

[0071] S3: Compound enzyme conversion: take trehalose conversion solution, add glucoamylase and com...

Embodiment 3

[0078] S1: Starch liquefaction: Mix starch and water to prepare starch slurry, then adjust the pH value of the starch slurry to 5.8, add α-amylase, mix well, heat up to 110°C for 8 minutes, and then flash evaporate to 99°C and laminar flow for 90min, control the DE value to 4, and inactivate the enzyme to obtain a liquefied liquid; the starch is tapioca starch; the mass concentration of the starch slurry is 30%; the amount of α-amylase added is 0.04% of the starch mass %.

[0079] S2: Trehalase conversion: take the liquefaction solution, add starch debranching enzyme and trehalose conversion enzyme, and enzymatically hydrolyze for 48 hours under the conditions of pH 6.5 and temperature 55° C. to obtain trehalose conversion solution. The trehalose inverting enzyme includes trehalose hydrolase and trehalose synthetase; the mass of the trehalose inverting enzyme is 3% of the starch mass.

[0080] S3: Compound enzyme conversion: take the trehalose conversion solution, add glucoam...

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Abstract

The invention discloses a production and preparation process of trehalose. The application adopts a high-temperature-resistant α-amylase, combines continuous high-temperature injection and flash laminar flow, and controls the DE value at the same time, so that the starch can be effectively liquefied, which is beneficial to improve conversion rate; in addition, this step can be carried out continuously without batch operation, which is beneficial to continuous large-scale production; the present invention obtains high-purity trehalose and gluconic acid through multiple transformations, and the main product trehalose is very easy to separate, and the purity of trehalose can reach As high as 99.81%, and the by-product gluconic acid is co-produced, which has high market value; the invention has high raw material utilization rate, coherent process, high product yield, improves enterprise output and reduces sewage discharge, energy saving and environmental protection, and is conducive to industrial production of high purity trehalose.

Description

technical field [0001] The invention relates to the technical field of preparation of trehalose, in particular to a production and preparation process of trehalose. Background technique [0002] Trehalose, also known as sucrose and mushroom sugar, is a non-reducing disaccharide composed of two glucose molecules. Trehalose is a typical stress metabolite, which can form a unique protective film on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure, and dehydration, effectively protecting the biomolecular structure from being destroyed, thereby maintaining Life processes and biological characteristics of living organisms. [0003] The purpose of trehalose production is to obtain high-purity trehalose products and related enzyme products. There are three main methods for preparing trehalose in production: one is to extract from biological cells; the other is to use microbial fermentation to produce; the third is to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/12C12P19/14C12P7/58C07C51/42C07C51/47C07C59/105C07H3/04C07H1/06
CPCC07C51/42C07C51/47C07H1/06C07H3/04C12P7/58C12P19/12C12P19/14C07C59/105
Inventor 楼志华丁红辉周立于令君
Owner JIANGSU OGO BIOTECH
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