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Glutamate oxidase biosensor and its preparation method and application

A glutamate oxidase and biosensor technology, which is applied in the field of electrochemical biosensors, can solve the problem that sodium glutamate detection has not been reported in literature, achieve good selectivity and stability, improve detection performance, and has wide application prospects Effect

Active Publication Date: 2021-11-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, based on PtNP@MXene-Ti 3 C 2 T x The biosensor prepared with glutamate oxidase and glutamate oxidase has not been reported in the literature for the detection of sodium glutamate in food, which belongs to the technical blank

Method used

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  • Glutamate oxidase biosensor and its preparation method and application
  • Glutamate oxidase biosensor and its preparation method and application
  • Glutamate oxidase biosensor and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Prepare a glutamate oxidase biosensor, the specific steps are as follows:

[0049] (1) First prepare PtNP@MXene-Ti 3 C 2 T x composite nanomaterials. Prepare 10mL of 1mg / mL MXene-Ti 3 C 2 T x The solution was sonicated for 10 minutes, 2.5 mL of 10 mg / mL chloroplatinic acid was added, and after 10 minutes of sonication, it was continuously reduced for 24 hours under the protection of nitrogen. The product was centrifuged at 9500 rpm for 10 min, washed with ethanol and lyophilized. The lyophilized product was formulated into a 0.5 mg / mL solution to obtain PtNP@MXene-Ti 3 C 2 T x nanocomposites. The scanning electron microscope image of the material is as figure 1 As shown, it can be clearly observed that the nano-platinum particles are attached to the sheet-like MXene-Ti 3 C 2 T x surface, indicating that PtNP@MXene-Ti 3 C 2 T x successful synthesis.

[0050] (2) Surface pretreatment of the working electrode. The glassy carbon electrode was polished to...

Embodiment 2

[0058] Prepare a glutamate oxidase biosensor, the specific steps are as follows:

[0059] (1) First prepare PtNP@MXene-Ti 3 C 2 T x composite nanomaterials. Prepare 10mL of 1mg / mL MXene-Ti 3 C 2 T x The solution was sonicated for 10 minutes, 2.5 mL of 10 mg / mL chloroplatinic acid was added, and after 10 minutes of sonication, it was continuously reduced for 24 hours under the protection of nitrogen. The product was centrifuged at 9500 rpm for 10 min, washed with ethanol and lyophilized. The lyophilized product was formulated into a 0.5 mg / mL solution to obtain PtNP@MXene-Ti 3 C 2 T x nanocomposites.

[0060] (2) Surface pretreatment of the working electrode. The glassy carbon electrode was polished to a mirror surface on a polishing cloth with 0.3 μm and 0.05 μm alumina powder in turn, rinsed with ultrapure water, and dried with nitrogen to obtain a pretreated glassy carbon electrode.

[0061] (3) The modification process of the working electrode is as follows: f...

Embodiment 3

[0069]Prepare a glutamate oxidase biosensor, the specific steps are as follows:

[0070] (1) First prepare PtNP@MXene-Ti 3 C 2 T x composite nanomaterials. Prepare 10mL of 1mg / mL MXene-Ti 3 C 2 T x The solution was sonicated for 10 minutes, 2.5 mL of 10 mg / mL chloroplatinic acid was added, and after 10 minutes of sonication, it was continuously reduced for 24 hours under the protection of nitrogen. The product was centrifuged at 9500 rpm for 10 min, washed with ethanol and lyophilized. The lyophilized product was formulated into a 0.5 mg / mL solution to obtain PtNP@MXene-Ti 3 C 2 T x nanocomposites.

[0071] (2) Surface pretreatment of the working electrode. The glassy carbon electrode was polished to a mirror surface on a polishing cloth with 0.3 μm and 0.05 μm alumina powder in turn, rinsed with ultrapure water, and dried with nitrogen to obtain a pretreated glassy carbon electrode.

[0072] (3) The modification process of the working electrode is as follows: fi...

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Abstract

The invention discloses a glutamic acid oxidase biosensor, a preparation method and an application thereof, and belongs to the technical field of electrochemical biosensors. The working electrode of this biosensor is made of PtNP@MXene‑Ti 3 C 2 T x solution, glutamate oxidase solution and chitosan solution modification, the preparation methods include: MXene‑Ti 3 C 2 T x The solution was mixed with chloroauric acid solution, sonicated and continuously reduced to obtain PtNP@MXene‑Ti 3 C 2 T x After the solution, it was drip-coated on the surface of the glassy carbon electrode, dried in a dust-free environment, and then modified with chitosan to glutamate oxidase on the surface, and refrigerated until completely dry. The enzyme biosensor of the invention can be used to detect the content of sodium glutamate (MSG) in food, and has high sensitivity, low detection limit and good anti-interference performance.

Description

technical field [0001] The invention relates to the technical field of electrochemical biosensors, in particular to a glutamic acid oxidase biosensor and its preparation method and application, which are used for quantitative detection of sodium glutamate. Background technique [0002] Glutamate is the main excitatory neurotransmitter in the central nervous system. Excessive glutamate-mediated neurotoxicity can lead to brain damage and cause neurodevelopmental disorders and neurodegenerative diseases. Glutamate usually exists in the form of monosodium glutamate (MSG) in food, because it can add umami to food and enhance palatability, it has become one of the additives widely used in the food industry. At present, methods for quantitatively measuring sodium glutamate mainly include acid titration, optical rotation, and high-performance liquid chromatography. Although these methods have good accuracy, they are complicated to operate, time-consuming, and require a lot of solven...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3272
Inventor 刘源刘静思樊玉霞姜水
Owner SHANGHAI JIAO TONG UNIV
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