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Kit for determining III C based on latex enhanced immunoturbidimetry and preparation and use methods of kit

An immunoturbidimetric and latex-enhanced technology, which can be used in biological testing, measuring devices, material inspection products, etc., and can solve problems such as poor selectivity

Pending Publication Date: 2020-10-30
ANHUI DAQIAN BIO ENG LIMITED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemiluminescence is a sensitive and simple method, but it is poorly selective and responds to a series of compounds rather than to a single compound

Method used

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  • Kit for determining III C based on latex enhanced immunoturbidimetry and preparation and use methods of kit
  • Kit for determining III C based on latex enhanced immunoturbidimetry and preparation and use methods of kit
  • Kit for determining III C based on latex enhanced immunoturbidimetry and preparation and use methods of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A kit for determining III C based on latex-enhanced immunoturbidimetric method in this embodiment includes reaction buffer R1 and latex reagent R2.

[0054] The components and corresponding contents included in the reaction buffer R1 are: tris buffer solution 5 g / L, magnesium chloride or potassium chloride 2 g / L, polyethylene glycol 1000 or polyethylene glycol 2000 10 g / L, Proclin300 1mL / L, BSA3 g / L, Tween-800.5mL / L, the solvent is purified water, and the pH is 7.1.

[0055] The latex reagent R2 includes at least two latex reagent composition solutions formed by coupling III C monoclonal antibodies with latex microspheres, each latex reagent composition solution includes a latex preservation solution and is stored in the latex preservation solution, and The corresponding III C mAb-labeled latex microspheres at a final concentration of 0.5 mg / mL. Among them, the ingredients and corresponding contents included in the latex preservation solution are: tris buffer solution ...

Embodiment 2

[0059] A kit for determining III C based on latex-enhanced immunoturbidimetric method in this embodiment includes reaction buffer R1 and latex reagent R2.

[0060] The components and corresponding contents included in the reaction buffer R1 are: glycine buffer or 2-(N-morpholine) ethanesulfonic acid buffer 12g / L, zinc chloride or calcium chloride 15g / L, polyethylene glycol 800030g / L, thimerosal 2mL / L, sucrose 14g / L, Triton X-100 2.5mL / L, the solvent is purified water, and the pH is 7.9.

[0061] The latex reagent R2 includes at least two latex reagent composition solutions formed by coupling III C monoclonal antibodies with latex microspheres, each latex reagent composition solution includes a latex preservation solution and is stored in the latex preservation solution, and The corresponding III C mAb-labeled latex microspheres at a final concentration of 1.0 mg / mL. Wherein, the composition and corresponding content that latex preservation solution comprises are: glycine buff...

Embodiment 3

[0065] A kit for determining III C based on latex-enhanced immunoturbidimetric method in this embodiment includes reaction buffer R1 and latex reagent R2.

[0066] The components and corresponding contents included in the reaction buffer R1 are: MOPSO buffer solution 8.06g / L, sodium chloride 5.4g / L, polyethylene glycol 600020g / L, Proclin3001.2 mL / L, BSA 6g / L, Tween-20 1.5mL / L, the solvent is purified water, the pH is 7.6.

[0067] The latex reagent R2 includes at least two latex reagent composition solutions formed by coupling III C monoclonal antibodies with latex microspheres, each latex reagent composition solution includes a latex preservation solution and is stored in the latex preservation solution, and The corresponding III C mAb-labeled latex microspheres at a final concentration of 0.75 mg / mL. Among them, the components and corresponding contents included in the latex preservation solution are: MOPSO buffer solution 4.3g / L, magnesium chloride 8.7g / L, glycine 16g / L, P...

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Abstract

The invention provides a kit for determining III C based on latex enhanced immunoturbidimetry. The kit comprises a reaction buffer solution R1 and a latex reagent R2; the reaction buffer solution R1 comprises a buffer solution, inorganic salt, a turbidity enhancer, a preservative, a stabilizer and a surfactant; the latex reagent R2 comprises at least two latex reagent composition solutions formedby coupling III C monoclonal antibodies and latex microspheres, and the mixing ratio of the composition solutions is 1: 1; in addition, each latex reagent composition solution further comprises a latex preservation solution and latex microspheres preserved in the latex preservation solution, and the latex preservation solution comprises a buffer solution, inorganic salt, a stabilizer, a preservative and a surfactant. The invention further provides preparation and use methods of the kit for determining III C based on latex enhanced immunoturbidimetry. The kit has the advantages of simple operation, fast detection speed, good selectivity, good specificity, high precision, and good repeatability and consistency.

Description

technical field [0001] The invention relates to the field of preparation of biological detection reagents, in particular to a kit for determining III C based on a latex-enhanced immune turbidimetric method and a preparation method thereof. Background technique [0002] During the development of liver disease, liver fibrosis will be experienced. With the continuous aggravation of liver fibrosis, a large number of liver cells will be shed and necrotic. At the same time, the structure of liver lobules will gradually become distorted and heavier, eventually leading to liver cirrhosis. happened. Therefore, clinically timely and effective diagnosis and treatment for patients with liver disease can greatly reduce the symptoms of patients and avoid further deterioration of the condition. Histopathological examination is the gold standard for clinical diagnosis of liver fibrosis. Although the pathological examination is highly accurate, it will inevitably cause certain trauma to the...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/543
CPCG01N33/6893G01N33/6887G01N33/577G01N33/54346G01N2333/78G01N2800/085
Inventor 芮双印任传伍
Owner ANHUI DAQIAN BIO ENG LIMITED