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Preparation method of high-content zeaxanthin

A technology with high content of zeaxanthin, applied in the field of biochemistry, can solve the problems of poor absorption of zeaxanthin, low bioavailability, and low product purity

Active Publication Date: 2020-11-03
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In nature, zeaxanthin mainly exists in corn bran, and the cost of extraction is very high; lutein can be converted into common zeaxanthin by chemical translocation, but the product contains about 15-30% lutein, The low purity of the product leads to poor absorption and low bioavailability of zeaxanthin in the body. In order to improve the bioavailability of zeaxanthin, increase the purity, thereby improving its transformation and utilization in the body

Method used

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preparation example Construction

[0021] A preparation method for high-content zeaxanthin, comprising the following steps:

[0022] a. Mix zeaxanthin crystals with an organic solvent to obtain a mixed solution; the organic solvent is any one or more of acetone, methylene chloride, and ethyl acetate solvent, and the volume of the organic solvent and the weight ratio of zeaxanthin crystals are ml / g= 5-100, the preferred ratio is 10-20.

[0023] Wherein, zeaxanthin is zeaxanthin extracted from lutein or its fatty acid conversion or other products. The HPLC detection content of zeaxanthin is between 10%-70%, preferably between 40%-60%.

[0024] b. Heat the mixed solution in step a to 40-90° C. in an inert gas environment, stir to form a mixed solution, and keep warm for 0.5-5 hours.

[0025] c. Cool down the mixed solution in step b to 0-25° C., keep the temperature for 1-5 hours, and separate to obtain crystals; the separation method is a conventional separation method.

[0026] d. Vacuum-dry the crystals obta...

Embodiment 1

[0028] Weighing 10g of zeaxanthin crystals obtained by the saponification of marigold oleoresin with a HPLC detection content of 20%, mixed with 200ml of dichloromethane, and stirred and mixed at 20°C. After the dissolution was completed, the mixture was heated to 40°C and reacted at this temperature. After reacting for 0.5h, the temperature was lowered to 0°C, kept for 1h and then filtered. After the crystals were vacuum-dried, the content of zeaxanthin crystals was determined to be 81.36% by HPLC.

Embodiment 2

[0030] Weigh 5g of zeaxanthin crystals obtained by lutein crystals, and the HPLC detection content is 60.12%, mix with 100ml ethyl acetate, and stir and mix at 25°C. After the dissolution was completed, the mixture was heated to 80°C and reacted at this temperature. After stirring for 3 hours, the temperature was lowered to 15°C, kept for 4 hours and then filtered. After the crystals were dried in vacuum, the content of zeaxanthin crystals was determined to be 82.33% by HPLC.

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Abstract

The invention discloses a preparation method of high-content zeaxanthin, which belongs to the field of biochemistry and comprises the following steps: a, mixing zeaxanthin crystals with an organic solvent to obtain a mixed solution; b, heating and stirring the mixed solution obtained in the step a in an inert gas environment to obtain a mixed solution; c, cooling the mixed solution in the step b,keeping the temperature for a period of time, and separating to obtain crystals; and d, drying the crystal obtained in the step c in vacuum, and removing the organic solvent to obtain the high-contentzeaxanthin crystal. According to the method, the purity of zeaxanthin can be remarkably improved, zeaxanthin crystals with the liquid phase content of 80% or above can be obtained, and the bioavailability of zeaxanthin is improved.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a preparation method of high-content zeaxanthin. Background technique [0002] Lutein and zeaxanthin are the only two carotenoids present in the human retina. They are very important for the treatment and prevention of age-related macular degeneration (ARMD), and can effectively protect the eyes from AMRD and the resulting blindness. Since zeaxanthin is fully conjugated, it provides somewhat better protection than lutein against light-induced damage. In fact, some studies in the mid-to-late 1980s also proved that zeaxanthin is mainly in the small area in the center of the macula of the human eye. If it leaves the concave part concentrically and approaches the outer periphery of the macula, the amount of zeaxanthin gradually decreases. The amount of lutein gradually increases, and in the periphery of the macula, lutein is the main yellow pigment. [0003] In nature, zeaxanthin mainly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
CPCC07C403/24
Inventor 安晓东王浩亮连运河高伟吕兴娜
Owner CHENGUANG BIOTECH GRP CO LTD
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