A kind of Fusarium oxysporum and its fermentation method and application
A technology of Fusarium oxysporum and the genus Fusarium, applied in the field of bioengineering, can solve problems such as endangering human and livestock health and safety, enhancing pests, pathogenic bacteria resistance, and reducing control effects, achieving good control effects and good development and application foreground effect
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Embodiment 1
[0038] This example provides a field test of the fermentation broth of Fusarium oxysporum to prevent and control melon powdery mildew, and the specific test operation is described as follows.
[0039] The application time of the field test was May 2018. The test site was located in a melon greenhouse in Tongzhang Village, Yangling, Shaanxi Province. There were 18 test plants, all of which were mature seedlings, 1.7 meters high, covering an area of about 10 square meters, and had good soil fertility. The treatment group sprayed with the fermentation broth of Fusarium oxysporum in the test facility was the test group, and the water spray treatment group was the control group. There were 2 treatments in total, with 3-4 plants in each treatment, and 10 leaves per plant were investigated. Use a manual sprayer to spray once, so that the front and back of the leaves are evenly covered with the liquid, and at the same time, the leaves near the blade are sprayed with the liquid. The ...
Embodiment 2
[0056] The examples provide a field test of the fermentation broth of Fusarium oxysporum for preventing and controlling grape downy mildew, and the specific test operation is described as follows.
[0057] The field test application time was July 2019. The test site was located in the grape germplasm resource base of Xintiandi Agricultural Demonstration Science and Technology Park, Yangling Demonstration Zone, Xianyang City, Shaanxi Province. The test plants were red globe grapes. The test is divided into three groups: the Fusarium oxysporum fermentation broth stock solution treatment group of the present invention (S fr1 18.2), the Fusarium oxysporum fermentation broth of the present invention is added with auxiliary agent treatment group (S fr1 18.2 + adjuvant) and the clear water control group, each group was set up with 3 repetitions, and 20 leaves were selected for each repetition for the test. During the test, leaves were randomly selected, the disease index was investi...
Embodiment 3
[0072] This example provides an indoor test of the fermentation broth of Fusarium oxysporum preventing and controlling the botrytis botrytis pathogen, and the specific test operation is described as follows.
[0073] In October 2019, grape fruits suffering from botrytis were collected from the grape germplasm resource base of Xintiandi Agricultural Demonstration Science and Technology Park, Yangling Demonstration Zone, Xianyang City, Shaanxi Province, and cultivated on PDA plates. Mildew pathogens. Get three 50mL fermentation broths of the Fusarium oxysporum (S fr1 18.2) Do the following: the first fermentation broth, centrifuge at 14000r / min for 10min, take the supernatant as sample A; the second fermentation broth, centrifuge at 14000r / min for 10min, take the precipitate and add 80% acetone aqueous solution to fully stir and extract, and extract. After filtration and concentration, after the acetone is fully volatilized, it is combined with the centrifugal supernatant, whic...
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