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Method and device for measuring temperature of biological tissue or cell

A technology of biological tissues and cells, applied in the field of temperature sensing, can solve the problems of poor controllability and achieve the effect of large scope and strong scalability

Active Publication Date: 2022-04-08
ZHEJIANG LAB +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these studies, the temperature sensing units are randomly located in cells or between cells, and the controllability is poor.

Method used

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  • Method and device for measuring temperature of biological tissue or cell
  • Method and device for measuring temperature of biological tissue or cell
  • Method and device for measuring temperature of biological tissue or cell

Examples

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Embodiment 1

[0034] This embodiment takes the implementation of the present invention to realize temperature sensing at a specific location in a cell as an example. The first laser is a 1064nm single-mode laser, the objective lens 8 is a microscopic objective lens with a high numerical aperture NA=0.9, and diamond particles with a diameter of about 40nm containing NV color center defects are selected as the temperature sensitive unit.

[0035] image 3 It is the resonance spectrum of the electron spin in the ground state of the diamond NV color center defect and the change curve of the energy level corresponding to the resonance line with temperature, image 3 The figure on the left shows the resonance line of the ground state of the NV color center. This line has two peaks. The frequency corresponding to the median line D of the peak is related to temperature. The relationship curve is as follows image 3 As shown in the figure on the right, as the temperature increases, D decreases, and...

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Abstract

The invention discloses a method and a device for measuring the temperature of biological tissues or cells. The micro-nano-sized diamond particles containing spin defects are introduced into biological tissues by a biochemical method, or the nano-sized diamond particles containing spin defects are introduced into the biological tissue by biochemical methods. The diamond particles are introduced into cells by biochemical methods, and the energy level change of the diamond defect spin ground state is detected by microwave resonance or the fluorescence spectrum change corresponding to the energy difference between the excited state of the defect and the ground state is detected, and the temperature at the location of the diamond particles is obtained. Optical tweezers precisely control the position of diamond particles in biological tissue or cells, and then obtain the temperature of different positions in biological tissue or cells. The method and device of the present invention can realize the real-time dynamic temperature tracking detection of the target biological tissue or cell at the micro-nano level.

Description

technical field [0001] The invention relates to the field of temperature sensing, in particular to a method and device for measuring the temperature of biological tissues or cells. Background technique [0002] Temperature is a fundamental physical parameter regulated by the body, and while it is important, scientists know very little about how it varies within and between cells. The temperature difference between cells in the body is only a few degrees at most, but researchers now suspect that such small differences can change the chemical properties and function of cells, or help doctors shut down the growth of tumors. Temperature affects several processes in cells, from gene expression to protein-protein interactions. Use intracellular temperature to sort healthy and diseased cells, or to regulate cellular processes by altering cell temperature. Measuring small changes in temperature between different parts of the cell is therefore needed to reveal how heat is generated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01K11/00
CPCG01K11/006
Inventor 李翠红陈杏藩傅振海高晓文蒋静胡慧珠
Owner ZHEJIANG LAB