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Construction method of acute hyperuricemia mouse model

A hyperuricemia and mouse model technology, which is applied in animal husbandry and other fields, can solve the problems of cumbersome operation, large individual differences in models, and long time-consuming induction of mouse models, so as to reduce the time of model building, shorten the time of model making, The effect of serum uric acid level stabilization

Active Publication Date: 2020-11-06
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned shortcomings and deficiencies of the prior art, the present invention provides a method for constructing a mouse model of acute hyperuricemia renal damage by using hypoxanthine, potassium oxonate and hairtail as modeling agents, which solves the problem of induced The mouse model takes a long time, the operation is cumbersome, and the technical problems of large individual differences between models

Method used

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  • Construction method of acute hyperuricemia mouse model
  • Construction method of acute hyperuricemia mouse model
  • Construction method of acute hyperuricemia mouse model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The construction of acute hyperuricemia mouse model, its steps are:

[0052] Selection of S1 model animals: 56 male Kunming mice of SPF grade, weighing 30-40 g, provided by Shanghai Jiesijie Experimental Animal Co., Ltd., the animal production license number is SCXK (Shanghai) 2018-0004, and the breeding environment temperature is 22-24°C, humidity 40-60%, mice free access to food and water.

[0053] S2 drug preparation: boil fresh hairtail (purchased from Wumart Supermarket) in water, dry in an oven, grind it into powder with a pulverizer, take the test mouse feed, pulverize it with a pulverizer, and mix it with hairtail powder in a ratio of 1:10 After fully mixing, use hot boiling water to make the mixed powder into a paste, put it into a feed press, press it into strips, put it in an oven and dry it for later use; both hypoxanthine and potassium oxonate use 0.5% carboxymethyl Sodium cellulose aqueous solution is prepared into a suspension for subsequent use;

[005...

Embodiment 2

[0055] Embodiment 2 relative body weight changes of mice

[0056] During the modeling test of embodiment 1, observe the general growth situation of mice every day and weigh regularly, calculate the relative body weight of mice, mouse relative body weight=every day body weight after administration / administration front body weight, then adopt Prism 6.07 Statistical software was used for data statistics, and the data were represented by (χ±S). One-way analysis of variance was used for comparison between groups, and Pfigure 1 shown.

[0057] Depend on figure 1 The results in the above shows that the body weight of the mice in the model group and the model+hairtail group showed an upward trend from the 1st to the 7th day, but compared with the blank group, there was no statistical difference (P>0.05), indicating that the hairtail feed was used as a production method. After 7 days of induction with the modeling agent, there was no significant effect on the body weight of the test m...

Embodiment 3

[0058] Example 3 Blood Sample Biochemical Index Changes

[0059] 2 hours after the mice were induced with the modeling agent in the modeling test of Example 1, blood samples were collected from the eyeballs of the mice in each group, and after standing at room temperature for 1-2 hours, the mice were treated at 4800rpm / min. Speed ​​centrifugation for 15min (using the GENESPEED X1 microcentrifuge of GENE Company), collect the serum, and measure the described The concentration of uric acid in the blood sample, the measurement results are shown in Table 1 below (in Table 1, compared with the blank group, * indicates P<0.05, ** indicates P<0.01).

[0060] The variation (χ±S, n=8) of each group mouse serum uric acid concentration in the embodiment 1 of table 1

[0061]

[0062] The percentage that the serum uric acid concentration SD value of each group mice descends in embodiment 1 is calculated as follows, model+hairtail group mouse serum concentration SD value descends (%)=(...

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Abstract

The invention relates to a construction method of an acute hyperuricemia mouse model. The method comprises the following steps: feeding a test mouse with a hairtail-containing feed every day, performing a 7-8-day modeling test; and in the last two days of the modeling test, respectively feeding the mouse with hypoxanthine and oteracil potassium every day. The method has the beneficial effects that: according to the construction method of the acute hyperuricemia mouse model, due to the fact that hypoxanthine, oteracil potassium and hairtail are adopted as the combination of a modeling agent, compared with the prior art, the model similar to the human body hyperuricemia attack process can be prepared through the synergistic effect of multiple machine modeling agents, and therefore the modeling time is shortened.

Description

technical field [0001] The invention relates to the technical field of mouse induction, in particular to a method for constructing an acute hyperuricemia mouse model. Background technique [0002] Hyperuricemia is a type of disease in which the metabolism of purine in the body is abnormal or the function of the kidneys to excrete uric acid is weakened, resulting in excessive production or decreased excretion of uric acid in the body, which in turn leads to a high level of serum uric acid. If the fasting blood uric acid level exceeds 420 μmol / L for men and 360 μmol / L for women on two different days, it is called hyperuricemia, and long-term hyperuricemia often induces kidney damage. The results of investigation and research show that with the improvement of people's living standards, the incidence of hyperuricemia is increasing day by day, and the cases of hyperuricemia renal damage are also increasing. The current drugs for the treatment of hyperuricemia have serious side e...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 高益槐李存勇邱安东郑春源
Owner ALPHA FUJIAN BIOTECH
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