Bacillus aryabhattai strain and application thereof

A technology of Bacillus arborii and strains, applied in the field of microbial control, can solve problems such as rampant diseases, pesticide residues, and drug resistance of pathogenic microorganisms, and achieve the effect of promoting healthy development and ecological environment protection

Active Publication Date: 2020-11-06
SHANDONG FOOD & FERMENT IND RES & DESIGN INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of soil-borne diseases is mainly through the use of chemical agents, and the long-term use of chemical agents will cause water source soil pollution, ecological balance damage, pesticide residues, secondary diseases rampant, and pathogenic microorganisms to develop drug resistance. serious consequences

Method used

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  • Bacillus aryabhattai strain and application thereof
  • Bacillus aryabhattai strain and application thereof
  • Bacillus aryabhattai strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Isolation and identification of embodiment 1 bacillus arzii

[0058] Using Fusarium oxysporum as the indicator bacteria, use the plate confrontation method to inoculate Fusarium oxysporum in the center of the petri dish, inoculate the strain to be screened at a distance of about 2.5cm from the center, culture it in a constant temperature incubator at 28°C, and observe in real time The existence and size of the inhibition zone, and finally a strain with strong antagonistic effect was screened out from the koji, named strain D1. After testing, it is a Gram-positive bacterium with a short rod shape, relatively thick, single arrangement, and no movement; on the plate, the colony is 2-3mm, light yellow, regular in shape, neat in edge, slightly without protrusions , opaque, without wrinkles (see figure 1 ). The 16S rDNA sequence of the strain D1 was sequenced, and the strain D1 was identified as Bacillus aryabhattai by combining the morphological characteristics of the bact...

Embodiment 2

[0063] Example 2 Bacillus aryabhattai (Bacillus aryabhattai) D1 enzyme activity assay

[0064] The above-mentioned Bacillus arborii D1 was cultured in a shaker flask of LB medium at 35° C. and 170 rpm for 48 hours, and the enzyme activities of different enzyme components in the fermentation broth were measured. The test results are shown in Table 1 below.

[0065] Table 1 Comparison of the activity of each enzyme activity component of Bacillus arborii D1

[0066]

[0067]

[0068] It can be seen from the above table that the Bacillus arzeiji D1 has various enzyme activities such as producing neutral protease, β-amylase, pullulanase, cellulase and phytase, which is beneficial to expand its application.

Embodiment 3

[0069] Example 3 Determination of Bacillus aryabhattai (Bacillus aryabhattai) D1 antibacterial ability

[0070] D1 strain was tested for its antibacterial activity against tobacco red spot disease by the confrontation culture method, and the activated pathogenic bacteria cake was punched out with a sterilized puncher, and the pathogenic bacteria cake was first connected to the center of a fresh PDA plate, and then the antagonistic bacteria Bacillus arseni D1 points were connected to the side of the bacterium cake, and the flat plate only inoculated with the bacterium cake was used as a control. Place them in a 28°C incubator upside down. After 3 days, the colony diameters of the treatment and control were measured, and the bacteriostatic rate was calculated. The results showed that the strain D1 had very significant antagonistic effects on Fusarium graminearum, Aureobasidium pullulans, Fusarium oxysporum and Rhizoctonia solani. The results are shown in Figure 2-5 and Table ...

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Abstract

The invention provides a bacillus aryabhattai strain and an application thereof, and belongs to the technical field of microbial control. Studies find that the screened bacillus aryabhattai has the capacity of efficiently inhibiting various plant fungal pathogenic bacteria such as fusarium graminearum, aureobasidium pullulans, fusarium oxysporum, rhizoctonia solani and the like, and a series of problems of chemical pesticide residues and the like cannot be generated after the bacillus aryabhattai is used, so that chemical agents can be less used or even not used, green organic crop products can be favorably produced, and healthy development and ecological environment protection of the agricultural industry are promoted, and the bacillus aryabhattai has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganism prevention and control, and in particular relates to a strain of bacillus arborii. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Soil-borne diseases refer to infectious diseases that occur in the roots or stems of plants and use soil as the medium of transmission, such as: vertical blight, sheath blight, bacterial wilt, wilt, blight, damping off, root rot, soft rot, root-knot nematodes , cyst nematodes, etc. Its harm is huge, resulting in a decline in the quality of crops, a substantial reduction in production or even a failure to harvest. In recent years, due to the long-term applica...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C05F11/08C12R1/07
CPCC12N1/20A01N63/22C05F11/08C12R2001/07C12N1/205
Inventor 黄艳红刘建军傅茂润张兴荣贺连智徐慧郑小嘎李峰
Owner SHANDONG FOOD & FERMENT IND RES & DESIGN INST
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