Preparation method of injectable anti-fracturing degradable supramolecular hydrogel

A technology of hydrogel and deionized water, which is applied in the fields of pharmaceutical formula, medical science, prosthesis, etc., and can solve the problem that hydrogel is not injectable

Active Publication Date: 2020-11-10
PEKING UNIV SHENZHEN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Recently, it has been reported that gellan gum was used as raw material to prepare anti-fracture hydrogels through a certain cross-linking reaction. The hydrogels gradually lost water during the compression process, and the compression strain reached more than 80% without fragmentation. After compression, it can absorb water and return to its original height. The interaction of a large number of functional groups in gellan gum is the main reason for the hydrogel’s anti-fracture properties. However, the hydrogel is not injectable and cannot be obtained by simple , made in a gentle way

Method used

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  • Preparation method of injectable anti-fracturing degradable supramolecular hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0035] (1) 1g chitosan is dissolved in 100mL volume fraction and is in the acetic acid aqueous solution of 1%, after dissolving, add 367 μ L methacrylic anhydride (MA) dropwise, react 24 hours under room temperature, obtain methacrylated chitosan ( CSMA).

[0036] (2) Dissolve 0.5g of 3-phosphonopropionic acid in 50mL of deionized water, add 5.33g of 2-(N-morpholine)ethanesulfonic acid monohydrate (MES monohydrate), add 0.9g of EDC (1-Ethyl -3-(3'-dimethylaminopropyl)carbodiimide) and 0.3g NHS (N-hydroxysuccinimide), fully dissolved and stirred at room temperature for 30 minutes.

[0037](3) Add the liquid after the reaction of chitosan and methacrylic acid in (1) step dropwise to the 3-phosphonopropionic acid solution in (2), stir while adding dropwise, after all dropwise add After reacting at room temperature for 24 hours, add 100mL deionized water to dilute, place the diluted solution in a 12-14kDa dialysis bag, and dialyze in deionized water at room temperature for 5 days...

Embodiment 2

[0040] (1) Dissolve 1 g of chitosan in 100 mL of 1% acetic acid aqueous solution by volume fraction, add 367 μL of methacrylic anhydride (MA) dropwise after dissolving, and react at room temperature for 24 hours.

[0041] (2) Dissolve 0.5g creatine phosphate in 50mL deionized water, add 5.33g 2-(N-morpholine)ethanesulfonic acid monohydrate (MES monohydrate), add 0.9g EDC (1-Ethyl-3-( 3'-dimethylaminopropyl)carbodiimide) and 0.3g NHS (N-hydroxysuccinimide), fully dissolved and stirred at room temperature for 30 minutes.

[0042] (3) Add the liquid after the reaction of chitosan and methacrylic acid in step (1) dropwise to the creatine phosphate solution in (2), stir while adding, and react at room temperature for 24 After 1 hour, add 100mL deionized water to dilute, place the diluted solution in a 12-14kDa dialysis bag, dialyze in deionized water at room temperature for 5 days, change the deionized water once every morning and evening, and keep stirring, freeze-dry after dialys...

Embodiment 3

[0045] (1) Dissolve 1 g of chitosan in 100 mL of 1% acetic acid aqueous solution by volume fraction, add 367 μL of methacrylic anhydride (MA) dropwise after dissolving, and react at room temperature for 24 hours.

[0046] (2) Dissolve 0.5g creatine phosphate in 50mL deionized water, add 5.33g 2-(N-morpholine)ethanesulfonic acid monohydrate (MES monohydrate), add 0.9g EDC (1-Ethyl-3-( 3'-dimethylaminopropyl)carbodiimide) and 0.3g NHS (N-hydroxysuccinimide), fully dissolved and stirred at room temperature for 30 minutes.

[0047] (3) Add the liquid after the reaction of chitosan and methacrylic acid in step (1) dropwise to the creatine phosphate solution in (2), stir while adding, and react at room temperature for 24 After 1 hour, add 100mL deionized water to dilute, place the diluted solution in a 12-14kDa dialysis bag, dialyze in deionized water at room temperature for 5 days, change the deionized water once every morning and evening, and keep stirring, freeze-dry after dialys...

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Abstract

The invention belongs to the technical field of polymer hydrogel, and particularly relates to a preparation method of injectable anti-fracturing degradable supramolecular hydrogel, which comprises thefollowing steps: carrying out methacrylic anhydride and phosphoric acid or phosphonic acid modification on natural polysaccharides, and compounding metal-containing particles to form supramolecular binding, thereby preparing the injectable anti-fracturing degradable hydrogel. The hydrogel obtained by the preparation method disclosed by the invention has anti-fracturing performance, is not brokenwhen being compressed to a certain degree (the compression strain is greater than 85%), and can be recovered to be close to the initial height after being soaked in an aqueous solution for a period oftime.

Description

technical field [0001] The invention belongs to the technical field of polymer hydrogel, and in particular relates to a preparation method of an injectable anti-fracturing degradable supramolecular hydrogel. Background technique [0002] Due to its good biosafety, biocompatibility, degradability, extracellular matrix-like structure and other characteristics, natural polysaccharide hydrogel has obvious advantages in the delivery of active substances and living cells, material exchange, tissue repair, etc., and is widely used. It is used in the research of tissue repair materials. Endowing natural polysaccharide hydrogel with injectability will make it have the characteristics of minimally invasive implantation and precise filling, which is convenient for surgical implantation, reduces surgical wounds and reduces the risk of infection. [0003] At present, the preparation method of injectable hydrogel is mainly to introduce physical or chemical cross-linking reactions in poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L5/08C08L5/04C08K3/22C08K3/26C08B37/08C08B37/04A61L27/44A61L27/50A61L27/52
CPCC08J3/075C08B37/003C08B37/0072C08B37/0084A61L27/446A61L27/50A61L27/52A61L2400/06C08J2305/08C08J2305/04C08K2003/222C08K2003/2224C08K2003/2296C08K2003/267C08K2003/2275C08K2201/011C08L5/08C08L5/04
Inventor 陈英奇康斌曾晖
Owner PEKING UNIV SHENZHEN HOSPITAL
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