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Phospholipid-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalane compound and SARS-CoV2 subunit vaccine

A subunit vaccine and vaccine adjuvant technology, applied in the field of biopharmaceuticals, can solve problems such as strong local irritation, difficult cellular immune response, and inflammatory response at the vaccination site

Active Publication Date: 2021-04-27
安徽省力瑞投资管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the particles of traditional aluminum adjuvants exist in gel form. Although they can activate Th2 cells to secrete IL-4 and promote Th2 humoral immune response to produce antibodies, it is difficult to effectively induce cellular immune response and cannot promote the body to produce cytotoxic T cells. cell
In addition, another prominent weakness of traditional aluminum adjuvants is that they are highly irritating and often lead to inflammatory reactions at the inoculation site.
[0006] In summary, aluminum salts, as vaccine adjuvants, need to be further developed and optimized in terms of safety and immunostimulatory efficacy

Method used

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  • Phospholipid-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalane compound and SARS-CoV2 subunit vaccine
  • Phospholipid-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalane compound and SARS-CoV2 subunit vaccine
  • Phospholipid-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalane compound and SARS-CoV2 subunit vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: Construct SPLAN and SCV2 subunit vaccine with SPC / DOTAP / Squalene and aluminum hydroxide nanoparticles

[0069] Take an appropriate amount of lipid materials (SPC and DOTAP) and Squalene (mass ratio of SPC / DOTAP / Squalene = 10:10:1), put them in a pear-shaped flask, add chloroform (the amount used is 3 times the weight of the lipid materials) to dissolve , room temperature, with 90rpm (90 rev / min) rotary evaporation, remove all organic solvents, form SPC / DOTAP / Squalene film.

[0070] According to AN / lipid material (SPC and DOTAP)=10:1 (mass ratio), add the aluminum hydroxide nanoparticle (AN, 1%w / v) aqueous solution (10mM HEPES buffer that contains average particle diameter 80 nanometers) to above-mentioned flask solution, pH=7), and rotated at 120 rpm for 1 hour to fully hydrate the SPC / DOTAP / Squalene film to form SPLAN.

[0071] Then the flask was transferred to a constant temperature electromagnetic stirrer, an electromagnetic stirrer was added, and at r...

Embodiment 2

[0086] Embodiment 2: Construct SPLAN and SCV2 subunit vaccine with SPC / DOTAP / Squalane and alumina nanoparticles

[0087] Take an appropriate amount of lipid materials (SPC and DOTAP) and Squalene (SPC / DOTAP / Squalane=15:10:1, w / w), put them in a pear-shaped flask, add chloroform (3 times the weight of lipid materials) to dissolve, At room temperature, the organic solvent was removed by rotary evaporation at 90 rpm (90 revolutions / minute) to form a SPC / DOTAP / Squalane film.

[0088] According to AN / lipid material (SPC and DOTAP=8:1 (mass ratio), add aluminum oxide nanoparticles (AN, 1% w / v) aqueous solution (10 mM HEPES buffer solution) containing an average particle size of 50 nm to the above flask , pH=7), rotate at 120 rpm for 1 hour to fully hydrate the SPC / DOTAP / Squalane film to form SPLAN.

[0089] Then the flask was transferred to a constant temperature electromagnetic stirrer, an electromagnetic stirrer was added, and at room temperature and 200rpm stirring conditions, a...

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Abstract

The invention belongs to the field of biological preparations, and discloses a phospholipid bilayer-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalene compound and a SARS-CoV2 subunit vaccine. The VADS uses aluminum nanoparticles as a carrier, and the surface of the aluminum nanoparticles is covered with a phospholipid bimolecular layer, and squalane is loaded between the phospholipid molecular layers; the present invention also coats the aluminum nanoparticle VADS with phospholipids loaded with squalene. and SARS-CoV2 antigen to construct a SARS-CoV2 subunit vaccine; the SARS-CoV2 subunit vaccine of the present invention has high safety and strong immune induction effect, and can induce the body to produce a high level of SCV2 antigen by injection or mucosal route vaccination Specific antibodies and cytotoxic T cells.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a phospholipid-coated aluminum nanoparticle vaccine adjuvant-delivery system loaded with squalene compound and a SARS-CoV2 subunit vaccine. Background technique [0002] Currently, pneumonia caused by SARS-CoV2 (COVID-19, 2019 novel coronavirus) is spreading rapidly around the world and has become an infectious disease pandemic that seriously threatens human life. The global economic loss caused by the prevention and control of the COVID-19 epidemic has also been immeasurable. Although researchers are working day and night to develop a vaccine against SARS-CoV2, there is still no vaccine formulation that can be widely administered. In this urgent situation, developing a safe and effective SARS-CoV2 vaccine as soon as possible to protect susceptible populations is of great significance for ensuring human safety, maintaining social stability, and restoring economic deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/39A61K39/215A61K39/385A61P31/14
CPCA61K39/12A61K39/385A61K39/39A61K2039/55505A61K2039/55511A61K2039/57A61K2039/60A61P31/14C12N2770/20034
Inventor 王理想许小锋白宗胜王旭之陈贤碧
Owner 安徽省力瑞投资管理有限公司