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Yeast with high yield of isoamyl acetate and its application

A technology of isoamyl acetate and yeast, applied in the field of development of non-Saccharomyces cerevisiae

Active Publication Date: 2022-04-01
WULIANGYE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is: there is no problem that the pure culture strain of K.turicensis yeast is used for solid-state fermentation or related industries

Method used

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  • Yeast with high yield of isoamyl acetate and its application
  • Yeast with high yield of isoamyl acetate and its application
  • Yeast with high yield of isoamyl acetate and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of high isoamyl acetate-producing yeast strains

[0035] Separation: The sampling cellar is the cellar that has been continuously fermented for more than 5 years in the production workshop of Yibin Wuliangye Co., Ltd. Sampling was taken at 12 days of fermentation, and a special sampler (hollow double-layer stainless steel sleeve) was inserted into the cellar, and the grains from 5 sampling points between 0.5m-1.5m from the surface of the cellar, the center and the surrounding area were taken, and put into In a sterile sampling bag, the ice box was transported back to the laboratory, and the yeast strains were isolated according to the following method within 1 hour after sampling: Dilute the fermented grain sample with sterile water 1000, 10000, and 100000 times respectively, and spread it on the xylose instead Glucose YPD medium (formulation: 1% yeast extract, 2% peptone, 2% xylose, 2% agar, sterilized at 121° C. for 20 min) with ...

Embodiment 2

[0037] Embodiment 2 Physiological and biochemical characteristics of solid-state fermentation high-yielding isoamyl acetate yeast of the present invention

[0038] Cultured on L-YPD agar medium at 28°C for 2 days, observed the colonies and cell morphology; Disaccharide, cellobiose, lactose, and melezitose are the only carbon sources in the YNB medium to cultivate yeast, observe its growth, and evaluate its ability to assimilate sugar; inoculate the yeast on the urea medium, and cultivate for 24 hours. The ability of yeast to utilize urea was evaluated according to whether the medium turned red. Inoculate lactic acid YPD medium (pH5.0) with 5mL of bacterial suspension, shake and culture at 100r / min at 20, 24, 28, 32, 36, and 40°C for 36h respectively, test its temperature tolerance, and inoculate with 5mL of bacterial suspension Into lactic acid YPD medium (pH 5.0) with ethanol content of 1, 2, 3, 4, 5, 6, 7, 8% respectively, shake culture at 28°C and 100r / min for 36h, and tes...

Embodiment 3

[0041] Example 3 Application of yeast of the present invention in the brewing of Luzhou-flavor liquor

[0042] First, use an inoculation needle to inoculate one loop of yeast strains preserved on the slant into lactic acid YPD liquid medium, culture at 28°C for 1 day, then centrifuge the obtained yeast suspension at 3000rpm for 5min at 25°C, and pour off the supernatant , add sterile water, mix well and centrifuge again under the same conditions, wash the cells, and make a yeast suspension with a concentration of about 0.5OD for future use.

[0043] The yeast was inoculated with 0.1% inoculum into grain grain grains and fermented for 70 days. Compared with the naturally fermented grain grains (control) without yeast inoculation, the main flavor substances produced by the yeast are shown in Table 2. Among them, the content of isoamyl acetate in grain grains increased from 0.007g / kg to 0.341g / kg, the content of β-phenylethanol increased from 0.014g / kg to 0.207g / kg, and the total...

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Abstract

The invention belongs to the technical field of development and utilization of non-saccharomyces cerevisiae, and in particular relates to a yeast with high isoamyl acetate production and application thereof. Aiming at the problem that the purely cultured yeast strains of the genus Kazachstania turicensis have not been used for solid-state fermentation, the present invention provides a high-yielding isoamyl acetate yeast for solid-state fermentation, and the preservation number is CGMCC 19812. The yeast adapts well to the solid-state fermentation environment of grain and has a strong metabolism. Its main volatile flavor substances include 700 mg / kg distilled grains of isoamyl acetate, 19.8 mg / kg distilled grains of acetic acid, 307 mg / kg distilled grains of β-phenylethyl alcohol, n-propyl Alcohol 8mg / kg grains, isoamyl alcohol 27mg / kg grains, n-butanol 3mg / kg grains, methanol only 2mg / kg grains. The grain grain fermented grains fermented by the yeast showed obvious floral and fruity flavor characteristics. The invention provides a new fermenting yeast for the production of liquor with richer flavor by solid-state fermentation, broadens the means for regulating the flavor of liquor, and has important practical significance.

Description

technical field [0001] The invention belongs to the technical field of development of non-saccharomyces cerevisiae, and in particular relates to a yeast with high isoamyl acetate production and application thereof. Background technique [0002] Luzhou-flavor liquor adopts the traditional mixed-bacteria natural solid-state fermentation process. After several rounds of continuous fermentation for hundreds of years, a relatively stable group of functional microorganisms has gradually formed in the fermented grains. These functional microorganisms are important characteristic microorganisms in my country. Resources, especially the yeast resources among them, not only undertake the task of converting reducing sugar into ethanol, but compared with yeast in other fermentation systems, its more important value lies in the production of alcohol, ester, acid, ester and other flavor substances It affects the quality of liquor, participates in the co-metabolism of microbial groups through...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12G3/02C12R1/645
CPCC12G3/02C12R2001/645C12N1/145
Inventor 游玲赵东谭壹彭智辅乔宗伟郑佳
Owner WULIANGYE
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