Method for preparing yak skin micromolecular collagen peptide through continuous rotary evaporation desolvation and double enzymolysis

A technology of collagen peptide and double enzymatic hydrolysis, which is applied in the preparation method of peptide, chemical instruments and methods, animal/human protein, etc., can solve the problem that cowhide collagen peptide has not been improved, affects the quality of end products, and the cost of small molecule peptides Advanced problems, to achieve the effect of reducing the burden of filtration, shortening the time of enzymatic hydrolysis, and reducing production costs

Active Publication Date: 2020-11-17
青海瑞肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cowhide collagen peptides currently on the market, compared with fish skin collagen peptides, most products are yellowish in color and have a light fishy smell of cowhide, which has a certain impact on the taste of food. As an added raw material, it also affects the quality of the end product
Since the yield of collagen peptides produced by terrestrial vertebrates is lower than that of marine animals, and a large amount of unhydrolyzed debris is produced in the process of enzymatic hydrolysis, which increases the burden on filtration, the cost of producing small molecular peptides that are conducive to human absorption is quite high, so that the production of bovine collagen Many parts of the protein peptide have not been improved

Method used

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  • Method for preparing yak skin micromolecular collagen peptide through continuous rotary evaporation desolvation and double enzymolysis
  • Method for preparing yak skin micromolecular collagen peptide through continuous rotary evaporation desolvation and double enzymolysis
  • Method for preparing yak skin micromolecular collagen peptide through continuous rotary evaporation desolvation and double enzymolysis

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Embodiment 1

[0032] A method for preparing yak skin small-molecule collagen peptides by continuous rotary evaporation, desolvation, and double enzymatic hydrolysis, which includes (1) pretreatment of yak skin, (2) homogenization, (3) enzymatic hydrolysis, (4) centrifugation, (5) Decolorization, deodorization, desalination, (6) concentrated drying step, the specific steps are as follows:

[0033] (1) Pretreatment: Weigh 50g of fresh yak hide, chop it into 1×1cm blocks after depilation, add soda ash with a mass concentration of 5% to soak for 8 hours at room temperature according to the mass ratio of material to liquid at 1:5kg / kg, After soaking, wash the yak skin with water until it is neutral, then add the yak skin to the salt with a mass concentration of 5% at a mass ratio of 1:5kg / kg, and soak for another 8 hours. After the soaking, wash the yak skin with water Neutral to achieve the purpose of degreasing and removing impurities;

[0034] (2) Homogenization: add 0.05M acetic acid soluti...

Embodiment 2

[0043] A method for preparing yak skin small-molecule collagen peptides by continuous rotary evaporation, desolvation, and double enzymatic hydrolysis, which includes (1) pretreatment of yak skin, (2) homogenization, (3) enzymatic hydrolysis, (4) centrifugation, (5) Decolorization, deodorization, desalination, (6) concentrated drying step, the specific steps are as follows:

[0044] (1) Pretreatment: Weigh 500g of fresh yak hide, chop it into 1×1cm blocks after depilation, add soda ash with a mass concentration of 3% at room temperature for 8 hours at a mass ratio of material to liquid of 1:5kg / kg, After soaking, wash the yak skin with water to neutrality, then add the yak skin to the mass concentration of 3% salt at a mass ratio of 1:5kg / kg, and soak for 8 hours again. After soaking, wash the yak skin with water Neutral to achieve the purpose of degreasing and removing impurities;

[0045] (2) Homogenization: add 0.05M acetic acid solution into 0.05M acetic acid solution wit...

Embodiment 3

[0054] A method for preparing yak skin small-molecule collagen peptides by continuous rotary evaporation, desolvation, and double enzymatic hydrolysis, which includes (1) pretreatment of yak skin, (2) homogenization, (3) enzymatic hydrolysis, (4) centrifugation, (5) Decolorization, deodorization, desalination, (6) concentrated drying step, the specific steps are as follows:

[0055] (1) Pretreatment: Weigh 20kg of fresh yak hide, chop it into 1×1cm blocks after depilation, add soda ash with a mass concentration of 5% at room temperature for 8 hours at a mass ratio of material to liquid of 1:5kg / kg, After soaking, wash the yak skin with water until it is neutral, then add the yak skin to the salt with a mass concentration of 5% at a mass ratio of 1:5kg / kg, and soak for another 8 hours. After the soaking, wash the yak skin with water Neutral to achieve the purpose of degreasing and removing impurities;

[0056] (2) Homogenization: add 0.05M acetic acid solution into 0.05M aceti...

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Abstract

The invention discloses a method for preparing yak skin micromolecular collagen peptide by continuous rotary evaporation desolvation and double enzymolysis. The method comprises the following steps of: (1) yak skin pretreatment, (2) homogenization, (3) enzymolysis, (4) centrifugal separation, (5) decolorization, deodorization and desalination, and (6) concentration and drying. The method is characterized in that the enzymolysis in the step (3) comprises the steps of primary enzymolysis, superfine treatment and secondary enzymolysis. Through combination of double enzymolysis and continuous rotary evaporation desolvation, the enzymolysis time can be greatly shortened, the process efficiency is improved, and the prepared yak skin collagen peptide is high in purity, colorless, odorless, smallin molecular weight and beneficial to direct absorption by human bodies.

Description

technical field [0001] The invention belongs to the technical field of collagen peptide extraction, and in particular relates to a method for preparing yak skin small-molecule collagen peptide by continuous rotary evaporation, desolvation and double enzymatic hydrolysis. Background technique [0002] Qinghai is the largest yak production base in the country, accounting for more than one-third of the total number of yaks in the country, and it is widely known as the "Yak Capital of the World". The unique plateau environment makes yaks have extremely strong immunity, strong ultraviolet radiation makes yaks a treasure house of sterile resources, and the natural way of stocking makes yaks have unique nutritional value and reliable safety. Therefore, the yak known as the "roof of the world" is an excellent raw material for extracting collagen peptides, which further increases the utilization value of yak hide and reduces the problem of slaughter "waste". [0003] Among the exist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/18C07K1/14C07K1/34C07K1/36C07K14/78
CPCC12P21/06C07K14/78Y02P20/10
Inventor 黄彪雷绍南马青林
Owner 青海瑞肽生物科技有限公司
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