Endophytic fungus ws01 of Gastrodia elata and its application in increasing the content of gastrodin in Gastrodia elata

A technology of endophytic fungi and Gastrodia elata, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., to achieve the effect of increasing the content of gastrodin

Active Publication Date: 2021-09-07
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are only a few reports on the research on the endophytic fungi of Gastrodia elata. For example, Liu Jingting reported the antibacterial activity of a strain of Gastrodia elata endophyte (Acremonium gastrodiae) on the pathogenic bacteria of Gastrodia elata, and Li Hongyu reported on the endophytic fungus of Gastrodia elata in Zhaotong, Yunnan ( Systematic Study on Secondary Metabolites of Aspergillus sp.T2-19) and Armillaria sp.
At present, there is no literature report on the research on the increase of gastrodin production by endophytic fungi in Gastrodia elata

Method used

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  • Endophytic fungus ws01 of Gastrodia elata and its application in increasing the content of gastrodin in Gastrodia elata
  • Endophytic fungus ws01 of Gastrodia elata and its application in increasing the content of gastrodin in Gastrodia elata
  • Endophytic fungus ws01 of Gastrodia elata and its application in increasing the content of gastrodin in Gastrodia elata

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Isolation and identification of endophytic fungus strain Ws01 from Gastrodia elata

[0040]In November 2017, obtain Gastrodia elata bulbs from Shenpo Mountain, Suining County, Hunan Province, and carry out surface disinfection: rinse the fresh Gastrodia elata bulbs with running water, then soak the cleaned bulbs with 75% ethanol for 3-5min, rinse with sterile water once, and use sodium hypochlorite ( Available chlorine 1.5%) soak for 1-2min, wash 3 times with sterile water, and dry the surface with sterile absorbent paper for later use. Under sterile conditions, take a tissue about the size of mung bean from the center of the corm of Gastrodia elata that has been sterilized on the surface, put it on a PDA solid medium, and culture it statically for 15 days at a temperature of 22-28°C to obtain an endophytic fungus of Gastrodia elata, namely Gastrodia elata endophyte Ws01 strain used in the present invention.

[0041] The Gastrodia elata endophyte Ws01 of the present in...

Embodiment 2

[0045] Application of endophytic fungus Ws01 in Gastrodia elata to increase gastrodin content in Gastrodia elata

[0046] 1. Select the endophytic fungus Ws01 strain we isolated from Gastrodia elata, which is classified as Phlebiopsis sp. Ws01, and is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation date is June 2020 11th, deposit number: GDMCC No.61055

[0047] 2. Activation and cultivation of strains in inclined test tubes. The endophytic fungus Phlebiopsis sp. (Phlebiopsis sp.) strain Ws01 of Gastrodia elata was inoculated into the slant test tube culture medium with mycelium blocks, and cultured at 25°C for 7-15 days to obtain slant strains;

[0048] Inclined test tube medium is PDA solid medium, 1000ml of PDA solid medium contains potato 200g, glucose 20g, agar 15g, add water to 1000ml, natural pH.

[0049] 3. The expanded culture is carried out by liquid shaking flasks to obtain seed liquid. Put 50ml of liquid shake flask seed c...

Embodiment 3

[0058] Embodiment 3: It is slightly different from steps 3-6 in Embodiment 2, but the rest are the same. Specifically:

[0059] 1. Select the endophytic fungus Ws01 strain we isolated from Gastrodia elata, which is classified as Phlebiopsis sp. Ws01, and is preserved in Guangdong Microbial Culture Collection Center (GDMCC), and the preservation date is June 2020 11th, deposit number: GDMCC No.61055

[0060] 2. Activation and cultivation of strains in inclined test tubes. The endophytic fungus Phlebiopsis sp. strain Ws01 of Gastrodia elata was inoculated into the slant test tube culture medium with mycelium blocks, and cultured at 25°C for 7 to 15 days to obtain slant strains;

[0061] Inclined test tube medium is PDA solid medium, 1000ml of PDA solid medium contains potato 200g, glucose 20g, agar 15g, add water to 1000ml, natural pH.

[0062] 3. Cultivation of Gastrodia elata bulbs: Lay the section wood (generally 30cm long and 7-8cm wide) on the bottom of the cellar bed (t...

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Abstract

The invention discloses an endophytic fungus Ws01 of Gastrodia elata and its application in increasing the content of gastrodin in Gastrodia elata. The classification of the endophytic fungus Ws01 of Gastrodia elata is named Phlebiopsis sp. Ws01 and is preserved in Guangdong Province Microorganism Culture Collection Center (GDMCC), the preservation date is June 11, 2020, and the preservation number is GDMCC No.61055. The Gastrodia elata endophyte Ws01 of the present invention can effectively increase the gastrodin content in Gastrodia elata, and the gastrodin content in Gastrodia elata containing the fungus can reach 0.451%, and its content is obviously higher than that in Gastrodia elata without the gastrodia elata endophyte Ws01 (0.304‑0.292%).

Description

Technical field: [0001] The invention belongs to the field of biological technology, in particular to an endophytic fungus of Gastrodia elata and its application and usage method. Background technique: [0002] Gastrodia elata Bl. is a rootless saprophytic plant in Orchidaceae. After the seeds germinate, they mainly rely on the Armillaria hyphae that invade the epidermis to obtain nutrition. Its bulb belongs to the precious medicinal material in China, has been used as medicine for more than two thousand years, and has the functions of treating migraine, antidepressant, sedative hypnosis and anti-inflammation. Gastrodin is one of the main medicinal active ingredients in Gastrodia elata. [0003] Gastrodia elata is not suitable for long-term storage after harvesting, it needs to be dried in time, otherwise it is easy to rot. Drying after steaming, drying after boiling, and direct drying of fresh products are the three most commonly used processing methods at present. During ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01C1/06C12R1/645
CPCA01C1/06C12N1/14C12N1/145C12R2001/645
Inventor 邹娟朱亚高佳乐王纯黄杰华王强刘胜贵伍贤进
Owner HUAIHUA UNIV
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