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Human-derived lactobacillus mucosae and application thereof

A mucosal lactobacillus, a technology for use, applied in the field of microbiology, can solve problems such as side effects, hyperlipidemia cannot be properly controlled, local or systemic drug allergy or immune resistance, etc.

Active Publication Date: 2020-11-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some drugs are currently used to treat obesity and obesity-related diseases, these drugs often have some side effects
Hyperlipidemia in some patients is not adequately controlled by diet and / or exercise therapy or by the use of therapeutic compounds
Local or systemic allergy or immune resistance to the drug may also occur with the use of the drug
[0004] At present, there is no effective method and medicine for the treatment and prevention of obesity and its related diseases with little side effects

Method used

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  • Human-derived lactobacillus mucosae and application thereof
  • Human-derived lactobacillus mucosae and application thereof
  • Human-derived lactobacillus mucosae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Example 1 Isolation and Identification of Mucosa Lactobacillus (Lactobacillus mucosae) A1 Strain

[0131] 1.1 Isolation of human Lactobacillus mucosae A1 strain

[0132] Cut 0.6g of GD02 children's feces samples after 105 days of nutritional intervention, add 30mL Ringer working solution (0.1% L-Cysteine) to the anaerobic incubator, and vortex until fully mixed. Centrifuge at 200 g for 5 min, and take 1 mL of the supernatant for serial dilution with Ringer (0.1% L-Cysteine) working solution. draw 10 -1 -10 -5 200 μL of the dilutions of these 5 dilution gradients were spread on the MRS agar plate, and each gradient was repeatedly coated 3 pieces, and cultured upside down under anaerobic conditions at 37° C. for 18 hours. Select a plate with more and clear colonies, randomly pick 200 single colonies growing on the plate, and perform streak purification twice on the MRS agar plate.

[0133]Inoculate the purified isolates of single bacteria into MRS broth medium, cultur...

Embodiment 2

[0157] The growth curve of embodiment 2 Lactobacillus mucosaeA1 bacterial strain

[0158] In this example, the growth curve of the Lactobacillus mucosae A1 strain in MRS medium was determined.

[0159] The specific method is: Streak and activate the Lactobacillus mucosae A1 strain frozen at -80°C on the MRS plate, pick a single clone and culture it overnight in 5mL MRS liquid medium, and transfer it to 150ml MRS liquid medium; mix well and divide into 10 15mL EP tubes, each tube has 14ml bacterial solution, take out a tube to measure OD about every 2 hours before the platform phase is observed 600 After that, take out a tube to measure OD about every 8 hours 600 value. The cultivation process was carried out in an anaerobic environment, and three repetitions were set for each growth curve measurement time point, and the measurement results were expressed as mean ± standard error.

[0160] The results of the growth curve measurement were as follows: image 3 As shown, the g...

Embodiment 3

[0161] Embodiment 3 Lactobacillus mucosaeA1 bacterial strain is to the ApoE under western diet environment - / - Alleviating effect of abnormal lipid metabolism in mice

[0162] 3.0 Experimental materials:

[0163] Mice: This example uses ApoE - / - C57BL / 6 male mice with genetic background, the mouse breeding process was completed in the SPF barrier of the Experimental Animal Center of Shanghai Jiao Tong University. ApoE (apolipoprotein E) is one of the important apolipoproteins in plasma, which is involved in lipid transport in blood.

[0164] Bacterial strain: the isolated Lactobacillus mucosae A1 strain described in Example 1

[0165] High-fat diet: Western diet with high fat and cholesterol, containing 21% fat and 0.15% cholesterol, purchased from Shanghai Fanbo Biotechnology Co., Ltd.

[0166] Normal maintenance feed: containing 4.8% fat, purchased from Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd.

[0167] 3.1 Experimental method:

[0168] 24 8-10 week old A...

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Abstract

The invention provides human-derived lactobacillus mucosae and application thereof, and particularly provides human-derived lactobacillus mucosae. The invention further provides a composition for treating and preventing obesity and related diseases thereof and a method for reducing weight and / or blood fat. Experiments show that the lactobacillus mucosae can reduce blood fat and atherosclerosis, and therefore can be applied to treatment and prevention of obesity and related diseases (such as cardiovascular diseases).

Description

technical field [0001] The present invention relates to the field of microorganisms. Specifically, the present invention relates to human-derived mucosal lactobacillus (Lactobacillus mucosae) and its use, including the application in the treatment and prevention of obesity and related diseases. Background technique [0002] Obesity is a systemic chronic metabolic disease, usually accompanied by abnormal lipid metabolism, imbalance of glucose homeostasis and chronic inflammation, etc. Intestinal flora is closely related to the occurrence of obesity, and an imbalanced intestinal flora is an important factor in inducing obesity. With the development of omics technology, those gut microbes related to the development of obesity have been continuously discovered and become potential effective targets for obesity treatment. [0003] Drug therapy is currently the main method for clinical treatment of obesity and obesity-related diseases (such as diabetes). Mechanisms of drug ther...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/04A61P3/06A61P9/00A61P3/10A23L33/135C12R1/225
CPCA61K35/747A61P3/04A61P3/06A61P9/00A61P3/10A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/11A23V2200/326A23V2200/3262A23V2200/328A23V2200/332
Inventor 张晨虹姜天翌赵立平
Owner SHANGHAI JIAO TONG UNIV
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