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Production method for enriching content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouting vegetables and increasing yield

A technology of isothiocyanate and aminobutyric acid, applied in fertilization methods, botany equipment and methods, seed and rhizome treatment, etc., can solve problems affecting the growth of broccoli sprouts and yield reduction, and achieve good health care The effect of function, low production cost and wide application range

Active Publication Date: 2020-11-27
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of abiotic stresses such as low temperature, low oxygen and sulfur-salt stress can increase the content of γ-aminobutyric acid and isothiocyanate in plants, but at the same time create a stress environment. Broccoli seeds are very sensitive to external adversity stress environments in the early germination stage. The presence of sensitive, strong abiotic stress environments will directly affect the growth of broccoli sprouts, resulting in greatly reduced yields
There is no documented solution to this problem

Method used

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  • Production method for enriching content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouting vegetables and increasing yield
  • Production method for enriching content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouting vegetables and increasing yield
  • Production method for enriching content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouting vegetables and increasing yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Broccoli seeds with germination ability were used as raw materials, soaked in hypoxic stress, and then germinated under constant temperature conditions under ultraviolet and zinc sulfate stress combined with exogenous melatonin to prepare enriched γ-aminobutyric acid and isothiocyanate High-yield broccoli sprouts with ester content. Specifically include the following steps:

[0028] (1) grain disinfection: select full grain, broccoli grain without damage by disease and insect, soak and disinfect with sodium hypochlorite, then clean up with distilled water;

[0029] (2) Hypoxic soaking: put the sterilized broccoli seeds in pure water, soak them at 30-35°C for 8-14 hours, and let in air during the soaking period, the ventilation rate is 1.5-5.0 L / min;

[0030] (3) Stress germination: put the soaked broccoli seeds in a constant temperature incubator at 30-35°C to germinate for 4-6 days, and irradiate with ultraviolet (UV-b) for 1-4 hours every 24 hours and spray the cultu...

Embodiment 2

[0035] Select broccoli seeds with full granules and no pests and diseases, soak in 1.5% sodium hypochlorite solution by volume for 15 minutes, wash with distilled water until neutral pH, and then soak in 4 times the amount (v / w) of pure water at 35°C During the soaking period, the air was continuously fed in for 8 hours, and the ventilation rate was 5.0 L / min; the soaked broccoli seeds were placed in a 30°C constant temperature incubator to germinate, and ultraviolet (UV-b) was irradiated every 24 hours for 1 hour during the germination period. Spray 2mmol / L zinc sulfate solution on 1 day, then spray 2mmol / L zinc sulfate and 100μmol / L melatonin mixed aqueous solution every 24h, the mass ratio of spraying amount to broccoli seeds is 6mL / g. On the 4th day of germination, Brassica sprouts with excellent growth and neat buds were obtained. The bud length per plant was 30mm, the fresh weight per plant was 40mg, and the content of γ-aminobutyric acid was 190mg / 100g fresh weight. The...

Embodiment 3

[0037] Select broccoli seeds with full granules and no pests and diseases, soak in 1.5% sodium hypochlorite solution by volume for 15 minutes, wash with distilled water until neutral pH, and then soak in 4 times the volume (v / m) of pure water at 32°C 10h, during the soaking period, the air was continuously fed, and the ventilation rate was 4.0L / min; the soaked broccoli seeds were placed in a 30°C constant temperature incubator to germinate, and ultraviolet (UV-b) irradiation was performed every 24h for 3h during the germination period. Spray 4mmol / L zinc sulfate solution on 1 day, then spray 4mmol / L zinc sulfate and 200μmol / L melatonin mixed aqueous solution every 24h, the mass ratio of spraying amount to broccoli seeds is 6mL / g. On the 6th day of germination, Brassica sprouts with excellent growth and neat buds were obtained. The bud length per plant was 40mm, the fresh weight per plant was 45mg, and the content of γ-aminobutyric acid was 140mg / 100g fresh weight. The content ...

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Abstract

The invention belongs to the technical field of deep processing of agricultural products, relates to a production method for enriching the content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouting vegetables and increasing the yield, and belongs to the technical field of processing of agricultural products. According to the production method in the invention, broccoli seeds withthe germination capacity are used as raw materials, soaked in low-oxygen stress, and then germinated through ultraviolet and zinc sulfate stress in combination with melatonin, so that gamma-aminobutyric acid and isothiocyanate are enriched, and the yield of the broccoli sprouting vegetables is increased; the content of gamma-aminobutyric acid in the product is 120-250 mg / 100 g fresh weight; and the content of isothiocyanate in the product is 20-500 mg / 100 g fresh weight. The production method in the invention has the characteristics of simple process, low production cost, high content of gamma-aminobutyric acid and isothiocyanate, high yield of broccoli sprouting vegetables and the like, and is suitable for production of health-care food-grade gamma-aminobutyric acid and isothiocyanate broccoli sprouting vegetables.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, and in particular relates to a production method for enriching the content of gamma-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing the yield. Background technique [0002] Broccoli is a Brassica crop, which has rich nutritional value. Usually, the growth process of Brassica sprouts mainly depends on the nutrients stored in the seeds. Generally, there is no need to fertilize in cultivation management, and the production operation is simple. To ensure its water supply, vermiculite cultivation methods are generally used now, and it is carried out under certain facility conditions or indoors. [0003] γ-aminobutyric acid is a non-protein amino acid widely present in animals and plants, and is an inhibitory neurotransmitter in mammals. γ-aminobutyric acid plays an important role in the energy metabolism of the human brain. It has various physi...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01C1/00A01C21/00C05G5/20C05G1/00
CPCA01G22/00A01C1/00A01C21/005C05D9/02C05G5/20C05F11/10
Inventor 尹永祺刘寅方维明徐金鹏田欣杨正飞
Owner YANGZHOU UNIV
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