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A production method for enriching the content of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing yield

An isothiocyanate-enriching technology for broccoli, which is applied in fertilization methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve problems such as affecting the growth of broccoli sprouts and reducing yield, and achieves good results. Health function, low production cost, and the effect of improving nutritional value

Active Publication Date: 2022-07-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of abiotic stresses such as low temperature, low oxygen and sulfur-salt stress can increase the content of γ-aminobutyric acid and isothiocyanate in plants, but at the same time create a stress environment. Broccoli seeds are very sensitive to external adversity stress environments in the early germination stage. The presence of sensitive, strong abiotic stress environments will directly affect the growth of broccoli sprouts, resulting in greatly reduced yields
There is no documented solution to this problem

Method used

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  • A production method for enriching the content of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing yield
  • A production method for enriching the content of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing yield
  • A production method for enriching the content of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Using broccoli seeds with germination ability as raw materials, after soaking under hypoxia stress, under constant temperature conditions and then under UV and zinc sulfate stress combined with exogenous melatonin germination, the enriched γ-aminobutyric acid and isothiocyanate were prepared. High-yielding broccoli sprouts with acid content. Specifically include the following steps:

[0028] (1) Disinfection of grains: select broccoli grains with full grains and no pests and diseases, soak and disinfect with sodium hypochlorite, and then clean them with distilled water;

[0029] (2) Hypoxic soaking: put the sterilized broccoli seeds in pure water, soak them at 30-35°C for 8-14 hours, and let in air during the soaking, and the ventilation rate is 1.5-5.0L / min;

[0030] (3) Stress germination: Place the soaked broccoli seeds in a constant temperature incubator at 30 to 35 °C for 4 to 6 days. During the germination period, 1 to 4 hours of ultraviolet (UV-b) irradiation is...

Embodiment 2

[0035] Select broccoli seeds with full grains and no pests and diseases, soak them in 1.5% sodium hypochlorite solution by volume for 15 minutes, wash with distilled water until neutral pH, and then put them in 4 times the volume (v / w) of pure water and soak at 35 ° C 8h, the air was continuously introduced during the soaking period, and the ventilation rate was 5.0L / min; the soaked broccoli seeds were placed in a 30°C constant temperature incubator to germinate, and 1h ultraviolet (UV-b) irradiation was performed every 24h during the germination period, and the germination first 2 mmol / L zinc sulfate solution was sprayed for 1 day, and then 2 mmol / L zinc sulfate and 100 μmol / L melatonin mixed aqueous solution was sprayed every 24 h. The ratio of spraying amount to broccoli grain mass was 6 mL / g. On the 4th day of germination, Brassica sprouts with excellent growth and neat buds were prepared. The sprouts per plant were 30 mm long, the fresh weight per plant was 40 mg, and the ...

Embodiment 3

[0037] Select broccoli seeds with full grains and no pests and diseases, soak them in 1.5% sodium hypochlorite solution by volume for 15 minutes, wash with distilled water until neutral pH, and then put them in 4 times the volume (v / m) of pure water and soak at 32 ° C 10h, the air was continuously introduced during the soaking period, and the ventilation rate was 4.0L / min; the soaked broccoli seeds were placed in a 30°C constant temperature incubator for germination, and 3h ultraviolet (UV-b) irradiation was performed every 24h during the germination period. 4 mmol / L zinc sulfate solution was sprayed for 1 day, and then 4 mmol / L zinc sulfate and 200 μmol / L melatonin mixed aqueous solution was sprayed every 24 h. The ratio of the spraying amount to the mass of broccoli grains was 6 mL / g. On the 6th day of germination, Brassica sprouts with good growth and neat buds were prepared. The sprouts per plant were 40 mm long, the fresh weight per plant was 45 mg, and the γ-aminobutyric ...

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Abstract

The invention belongs to the technical field of deep processing of agricultural products, and relates to a production method for enriching the contents of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing the yield, and belongs to the technical field of agricultural product processing. The invention uses broccoli seeds with germination ability as raw materials, soaked under hypoxic stress, and then subjected to ultraviolet and zinc sulfate stress combined with melatonin for germination, so as to enrich γ-aminobutyric acid and isothiocyanate and increase the concentration of glutathione. The yield of orchid sprouts. The content of γ-aminobutyric acid in the product is 120-250mg / 100g fresh weight, and the content of isothiocyanate is 20-500mg / 100g fresh weight. The invention has the characteristics of simple process, low production cost, high content of γ-aminobutyric acid and isothiocyanate, and high yield of broccoli sprouts, and is suitable for health food grade γ-aminobutyric acid and isothiocyanate Production of orchid sprouts.

Description

technical field [0001] The invention belongs to the technical field of deep processing of agricultural products, in particular to a production method for enriching the content of γ-aminobutyric acid and isothiocyanate in broccoli sprouts and increasing the yield. Background technique [0002] Broccoli is a Brassica crop, which has rich nutritional value. Usually, the growth process of Brassica sprouts mainly depends on the nutrients stored in seeds. Generally, no fertilization is required in cultivation management. Therefore, vermiculite cultivation is generally used in certain facilities or indoors. [0003] γ-aminobutyric acid is a non-protein amino acid, widely present in animals and plants, and is an inhibitory neurotransmitter in mammals. Gamma-aminobutyric acid plays an important role in the energy metabolism of the human brain, and has various physiological functions such as activating glucose metabolism in the brain, promoting the synthesis of acetylcholine, reducin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/00A01C1/00A01C21/00C05G5/20C05G1/00
CPCA01G22/00A01C1/00A01C21/005C05D9/02C05G5/20C05F11/10
Inventor 尹永祺刘寅方维明徐金鹏田欣杨正飞
Owner YANGZHOU UNIV
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