Unlock instant, AI-driven research and patent intelligence for your innovation.

Depolymerase with capability of degrading K64 capsule klebsiella pneumoniae extracellular polymeric substances

A Klebsiella, depolymerase technology, applied in the field of depolymerase, can solve the problem of difficult cleavage of Klebsiella pneumoniae extracellular polymers, etc.

Active Publication Date: 2020-12-01
SHANGHAI RUIZHOU BIOTECH CO LTD
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, phages have a very specific host spectrum, and the cleavage ability of Dpo42 and Dpo43 is also specific, and it is difficult for them to produce a satisfactory lysis effect on K64 capsule type Klebsiella pneumoniae extracellular polymer

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Depolymerase with capability of degrading K64 capsule klebsiella pneumoniae extracellular polymeric substances
  • Depolymerase with capability of degrading K64 capsule klebsiella pneumoniae extracellular polymeric substances
  • Depolymerase with capability of degrading K64 capsule klebsiella pneumoniae extracellular polymeric substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0081] 1. Experimental method

[0082] 1.1 Isolation and identification of strains

[0083] Klebsiella pneumoniae 2410 was isolated from Huashan Hospital Affiliated to Fudan University and kept in our laboratory as the host bacterium. Other clinical strains of Klebsiella pneumoniae were also obtained from Huashan Hospital. According to the method of Brisse (Wzi gene sequencing, arapid method for determination of capsular type for Klebsiella strains. J. Clin. Microbiol. 51, 4073–4078.), the strain capsular type was determined by wzi sequencing. These clinical isolates were cultured in LB medium at 37°C and stored at -80°C with the addition of glycerol.

[0084] 1.2 Phage isolation

[0085] Phages were isolated from biologically treated wastewater using Klebsiella pneumoniae clinical isolate 2410. Centrifuge the untreated water sample, infect Klebsiella pneumoniae 2410 strains in LB liquid medium, and culture overnight at 37°C. After centrifugation, the supernatant containi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biomedical treatment, in particular to a depolymerase with the capacity of degrading K64 capsule klebsiella pneumoniae extracellular polymeric substances. The depolymerase provided by the invention can be used for effectively decomposing the K64 capsular klebsiella pneumoniae extracellular polymeric substances, so that a medicine capable of killing the bacteria is in contact with the bacteria conveniently so as to exert the medicine effect.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a depolymerase capable of degrading extracellular polymers of K64 capsular Klebsiella pneumoniae. Background technique [0002] Klebsiella pneumoniae, a Gram-negative, encapsulated enteric bacterium, is the most common opportunistic pathogen causing a wide range of nosocomial infections including sepsis, pneumonia, urinary tract infections (UTIs) and Liver abscess. In recent years, Klebsiella pneumoniae has also emerged as a potential community-acquired pathogen and is an important cause of community-acquired liver abscess worldwide, especially in Asia. In the context of heavy antibiotic use, multidrug resistance of Klebsiella pneumoniae has increasingly emerged in hospitals over the past decade. Since 2013, Klebsiella pneumoniae has been recognized as a major member of carbapenem-resistant Enterobacteriaceae (CRE), with a multidrug-resistant phenotype, and as a class of antibiotic-r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/24C12N15/56C12N7/00C12N15/70C12N1/21A61K38/47A61K45/06A61P31/04C12R1/92C12R1/19
CPCC12N9/24C12N7/00C12N15/70A61K38/47A61K45/06A61P31/04C12N2795/10221C12N2795/10232A61K2300/00
Inventor 祝先潮何平李佳茵杜娟
Owner SHANGHAI RUIZHOU BIOTECH CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com